Diatoms are ubiquitous marine photosynthetic eukaryotes that are responsible for about 20% of global photosynthesis. Nevertheless, little is known about the redox-based mechanisms that mediate diatom sensing and acclimation to environmental stress. Here we used a redox-sensitive green fluorescent protein sensor targeted to various subcellular organelles in the marine diatom Phaeodactylum tricornutum, to map the spatial and temporal oxidation patterns in response to environmental stresses. Specific organelle oxidation patterns were found in response to various stress conditions such as oxidative stress, nutrient limitation and exposure to diatom-derived infochemicals. We found a strong correlation between the mitochondrial glutathione (GSH) redox potential (EGSH) and subsequent induction of cell death in response to the diatom-derived unsaturated aldehyde 2E,4E/Z-decadienal (DD), and a volatile halocarbon (BrCN) that mediate trophic-level interactions in marine diatoms. Induction of cell death in response to DD was mediated by oxidation of mitochondrial EGSH and was reversible by application of GSH only within a narrow time frame. We found that cell fate can be accurately predicted by a distinct life-death threshold of mitochondrial EGSH (−335 mV). We propose that compartmentalized redox-based signaling can integrate the input of diverse environmental cues and will determine cell fate decisions as part of algal acclimation to stress conditions.
Production of reactive oxygen species (ROS) in photosynthetic organisms occurs mainly in the chloroplast and mitochondria as a byproduct of oxygen-based metabolism (Halliwell, 2006). As ROS can be highly toxic molecules, their levels are tightly regulated by the antioxidant system that consists of ROS-scavenging enzymes and small molecules, such as glutathione (GSH) (Mittler, 2002; Foyer and Noctor, 2011). Although toxic ROS levels are controlled by the cellular antioxidant system to prevent oxidative damage, moderate ROS levels can be used as central secondary messengers that regulate signaling networks (Danon, 2002; D’Autréaux and Toledano, 2007; Jones and Go, 2010). The specificity of the ROS signal is achieved by variations in ROS levels, the specific chemical species and their intracellular localization (Foyer and Noctor, 2003; Gadjev et al., 2006; Møller and Sweetlove, 2010; Rosenwasser et al., 2013, 2014).
GSH, the most abundant low-molecular-weight thiol antioxidant, has a critical role in maintaining a reducing cellular thiol–disulfide balance and in detoxification of H2O2 via the ascorbate–GSH cycle (Rijstenbil and Wijnholds, 1996; Foyer and Noctor, 2011). In addition, GSH was recently suggested to have a pivotal role in regulating iron metabolism by maintaining the mitochondrial iron–sulfur clusters in yeast (Kumar et al., 2011). Oxidative stress that results from imbalance between ROS generation and antioxidant capacity is a common feature of cells exposed to environmental stress conditions. Under such conditions, electrons used for ROS detoxification are drawn, at least in part, from the cellular GSH pool, leading to an elevation of oxidized glutathione and a shift in the GSH redox potential (EGSH) toward oxidation (Meyer, 2008). Alterations in the EGSH as a result of ROS production can regulate the activation of many biological processes, such as transcription, post-translational modification and protein–protein interactions, by affecting the oxidation state of thiol groups in redox-sensitive proteins (Dietz, 2008; Meyer, 2008).
The development of fluorescent redox-sensitive biosensors such as the reduction-oxidation sensitive green fluorescent protein (roGFP) (Dooley et al., 2004; Hanson et al., 2004) allows in vivo quantitative and dynamic measurements of the cellular redox state and offers an advantage over other common destructive methods (for example, crude cell extracts). In vitro characterization of roGFP activity in plants, showed that roGFP reduction is mediated by glutaredoxin, which catalyzes the reversible electron flow between GSH and target proteins (Meyer et al., 2007). In contrast, the redox status of roGFP was not significantly affected by thioredoxin or other redox-active compounds such as nicotinamide adenine dinucleotide phosphate and ascorbate (Meyer et al., 2007; Gutscher et al., 2008). These in vitro observations were also tested in vivo and the specificity of the roGFP to detect the redox state of the GSH pool was validated in HeLa cells, using GSH biosynthesis inhibitors (Dooley et al., 2004) and in Arabidopsis mutants in GSH biosynthesis and reduction (Meyer et al., 2007; Rosenwasser et al., 2010). In addition, in vitro analysis showed that the fluorescence ratio data obtained from the roGFP is insensitive to alteration of the pH in physiological range (Schwarzländer et al., 2008). The above study points for the predominant interaction between the roGFP and the GSH pool and suggests that EGSH can be assessed in vivo in various cellular organelles by roGFP.
Diatoms are a highly predominant group of eukaryotic photosynthetic alga responsible for about 40% of marine photosynthesis (Nelson et al., 1995; Field et al., 1998). Consequently, diatoms have a central role in the biogeochemical cycling of important nutrients such as carbon and nitrogen. Diatoms can form massive blooms in the ocean that are controlled by abiotic stress, such as nutrient limitation and light regime, and by biotic interactions with grazers, viruses and through allelopathic interactions. During these interactions, an array of bioactive compounds (infochemicals) that have an important role in regulating cell fate are produced, and can shape population dynamics and composition (Pohnert et al., 2007). When diatom populations are subjected to grazing or nutrient stress, cells can rapidly induce the biosynthesis of diatom-derived oxylipins (DD) or volatile halocarbons (BrCN), which are bioactive signals that act as chemical defense mechanisms against grazing or as allelochemicals to suppress competing species (Ianora et al., 2004; Casotti et al., 2005; Vardi et al., 2006; Vanelslander et al., 2012). Recent reports suggest that diatom cells may utilize a Ca2+ induced NO-based system to monitor stressed cells within a population. In this system, the non-stressed cells monitor the level of DD concentrations produced by the stressed cells, thereby providing a stress surveillance system to monitor the stress level of the entire population (Vardi et al., 2006, 2008). Release of sublethal levels of DD can serve as an early warning protective mechanism, and lethal doses will initiate the cascade responsible for cell death and bloom termination. Recent evidences suggested that diatoms induce hallmarks of a programmed cell death (PCD)-like mechanism as a result of environmental stress such as iron limitation, culture age and exposure to infochemicals (Berges et al., 1998; Casotti et al., 2005; Vardi et al., 2008; Thamatrakoln et al., 2012). Nevertheless, very little is known about the role of ROS in mediating the perception of environmental stress conditions and possible regulation of cell fate during algal bloom dynamics.
We hypothesized that various environmental stress conditions may differentially affect the subcellular EGSH, which will consequently decode this redox perturbation into activation of specific signal transduction pathways that regulate cell fate. To examine this hypothesis, we monitored subcellular EGSH values in the model diatom Phaeodactylum tricornutum under steady-state and stress conditions by using diatom cells expressing the roGFP probe in various subcellular localizations. We provide compelling evidence for the specificity in diatom’s response to oxidative stress and multiple environmental cues by alteration of organelle-specific EGSH. Our results also show a strong correlation between early redox perturbation in the mitochondria and subsequent cell death, and reveal that cell death can be predicated based on a distinct mitochondrial EGSH threshold. These results may have important implications for better understanding the cellular mechanisms used by marine diatoms during acclimation to stress and the fate of blooms in the ocean.
Materials and methods
P. tricornutum, accession Pt1 8.6 (CCMP2561 in the Provasoli-Guillard National Center for Culture of Marine Phytoplankton) was purchased from the National Center of Marine Algae and Microbiota (NCMA, formerly known as CCMP; Bigelow, ME, USA). Unless otherwise specified, cultures were grown in f/2 media (882.4 μM NaNO3, 35.21 μM Na2HPO4•12 H2O, 1.83 μM FeCl3•6 H2O) (Guillard and Ryther, 1962) at 18 °C with 16:8 h light:dark cycles and light intensity of 80 μmol photons m−2 s−1 supplied by cool-white LED lights (Edison, New Taipei, Taiwan). All experiments were performed with exponentially growing cultures at ∼5 × 105 cells ml−1. Nutrient limitation experiments were done by washing the cells in nutrient-free media and resuspending them in f/2 without the appropriate nutrient (nitrogen or iron). For iron limitation, 1 μM of the efficient iron chelator desferrioxamine B was added.
Microscopy images where obtained on IX71S1F-3-5 motorized inverted Olympus microscope (Tokyo, Japan) equipped with a 60 × objective and filter systems for oxidized roGFP (ex:405/20 nm, em:525/50 nm), reduced roGFP, Annexin V, TUNEL and sytox staining (ex:470/40 nm, em:525/50 nm), and chlorophyll autofluorescence (ex:500/20 nm, em:650 nm LP). Images were captured using an EXi Blue (Q Imaging, Surrey, BC, Canada) camera.
Measuring roGFP oxidation
Using fluorescence microscopy, oxidized and reduced forms of roGFP were observed at excitation of 405 nm and 470 nm, respectively. In all, 525 nm emission intensities were collected and ratio images were created by dividing the ex:405, em:525 nm image by the ex:470, em:525 nm image, pixel by pixel using MATLAB, displayed in pseudocolor. Quantitative data were obtained using an Eclipse iCyt flow cytometer (Sony Biotechnology Inc., Champaign, IL, USA), equipped with 405 and 488 nm solid state air cooled lasers, both with 25 mW on the flow cell and with standard filter set-up, whereby roGFP was measured in the green channel (525/50 nm) following excitation at 405 nm (oxidized) and 488 nm (reduced). Leakage of chlorophyll autoflorescence to the green channel was subtracted by measurement of WT cells in parallel to roGFP transformants. Calibration of the fluorescence ratio arbitrary units was achieved by comparing the ratios of the roGFP fully reduced and fully oxidized states (30 min after treatment with 1 mM DTT or 200 μM H2O2, respectively). The degree of oxidation of roGFP and EGSH values were calculated according to Schwarzländer et al. (2008) using the Nernst equation and pH values of 6.9 for the cytoplasm and nucleus, 7.9 for the chloroplast (Anning et al., 1996) and 7.8 for mitochondria (Schwarzländer et al., 2008). Alterations of the estimated pH values will alter the calculated redox potential by 2.9 mV for every 0.05 pH units (Supplementary Figure S1). Kinetic analyses of degree of oxidation of roGFP were performed, cells were kept under light intensity of 20 μmol photons m−2 s−1 throughout the measurements.
GSH reduced ethyl ester (Sigma-Aldrich, Rehovot, Israel) was added to the cells at a final concentration of 1 mM.
Results and Discussion
Compartmentalized basal redox state in P. tricornutum
In order to map the in vivo spatial and temporal redox patterns in the model diatom P. tricornutum under steady state and in response to environmental stresses, the redox-sensitive GFP sensor was targeted to specific organelles, the cytoplasm (cyt-roGFP), the chloroplast stroma (chl-roGFP), the nucleus (nuc-roGFP) and the mitochondria (mit-roGFP) (Rosenwasser et al., 2014).
Ratiometric images derived from the division of the emitted fluorescence (measured at 525 nm) due to excitation at 405 nm (Figure 1a) and 488 nm (Figure 1b) are indicative of the in vivo oxidation level of roGFP (Figures 1c–e; Dooley et al., 2004; Hanson et al., 2004). Fluorescence ratios (ex:405, em:525 nm/ex:488, em:525 nm) obtained following oxidation by H2O2 were higher than in steady-state cells in all the examined subcellular compartments (Figures 1d and c). On the other hand, the fluorescence ratios of the images obtained after application of an external reductant (DTT) were only slightly lower than the steady-state ratios, indicating that, under steady-state conditions, all the examined compartments (cytoplasm, nucleus, mitochondria and chloroplast) have highly reduced microenvironments (Figures 1e and c). The dynamic range of the probe (Ratiooxidized/Ratioreduced), calculated based on flow cytometry measurements, was 6.8-9.3, similar to the that obtained from purified roGFP2 (Schwarzländer et al., 2008). These data demonstrate high in vivo signal-to-noise ratios and suggest that the roGFP probe can serve as an accurate sensor for subcellular EGSH in marine diatoms.
Based on the roGFP ratio data, the EGSH values in the different subcellular compartments can be estimated using the Nernst equation (Meyer and Dick, 2010). We assumed pH values of 6.9 for the cytoplasm and nucleus, 7.9 for the chloroplast based on measurements in coccolithophorids (Anning et al., 1996) and 7.8 for mitochondria based on measurements in plants (Schwarzländer et al., 2008). Importantly, despite the pH-independent characteristics of the roGFP sensor, alterations of the estimated pH values will alter the calculated redox potential by 2.9 mV for every 0.05 pH units (Supplementary Figure S1) as derived from the Nernst equation. The EGSH in the cytoplasm and nucleus were similar and ranged between –319 and –312 mV, while that of the mitochondria and chloroplast were more reduced, reaching –363 and –366 mV (Figure 1f). These results show that the GSH pool is kept under distinct, non-equilibrium reduced state in each subcellular compartment. Similar results describing the compartmentalization of the EGSH steady-state conditions were reported for mammalian cells and plants (Hanson et al., 2004; Hu et al., 2008; Schwarzländer et al., 2008; Rosenwasser et al., 2010; Dardalhon et al., 2012).
Organelle-specific response to oxidative stress in diatom cells
We further examined the differential response of the EGSH in the various organelles by kinetic measurements of oxidation and reduction of roGFP following H2O2 treatments (Figure 2a). All transformants harboring the roGFP probe oxidized in a dose-dependent manner and responded to physiologically relevant concentrations of H2O2 (Figure 2). However, saturation of the probe in each of the organelles was reached at different H2O2 concentrations. The cyt-roGFP reached saturation at concentrations of 50–80 μM H2O2, nuc-roGFP reached saturation at 80–100 μM H2O2, while the mitochondria and chloroplast probes saturated at 150 μM H2O2 (Figures 2b–e). Interestingly, we detected only minor oxidation (∼5%) between 0 and 50 μM H2O2 in the chloroplast, while a high oxidation level (>50%) was detected for 50 μM H2O2 treatment in all other roGFP transformants (Figures 2b–e). These data may indicate an organelle-specific antioxidant capacity to cope with oxidative stress and ROS-mediated stress conditions. We further utilized the reversibility property of the roGFP probe to monitor the in vivo oxidation–reduction patterns over time of exposure to oxidative stress. Once oxidized, the roGFP probe may be reduced back to its basal level. We termed this dynamic the recovery phase. Differential patterns of the recovery phase were observed in each of the examined subcellular compartments in response to a range of H2O2 concentrations. Although roGFP oxidation in the cytoplasm and nucleus recovered from exposure to 50–80 μM H2O2 (Figures 2b and d), the oxidation in the chloroplast only partially recovered and did not reduce back to the basal state (Figure 2c). A unique pattern of recovery was observed in the mitochondrial roGFP, which fully recovered by 4 h after the addition of up to 150 μM H2O2 (Figure 2e). Importantly, addition of 1 mM reduced GSH 30 min after the treatment with 150 μM H2O2 quickly reduced the probe back to its basal level in all organelles (Supplementary Figure S2), emphasizing the sensitivity and reversibility of the roGFP probe in its ability to detect perturbations in cellular GSH pool. Taken together, these data demonstrate the compartmentalized characteristics of the EGSH in different organelles within a diatom cell.
Early redox perturbation in response to oxidative stress can predict cell fate
Widespread documentation of PCD in diverse classes of phytoplankton, including chlorophytes, dinoflagellates, diatoms and coccolithophorids, in response to environmental stresses suggest an important evolutionary and ecological role for PCD in marine protists (Bidle and Falkowski, 2004; Franklin et al., 2006; Orellana et al., 2013). Nevertheless, the molecular mechanisms involved in PCD in phytoplankton are poorly understood. We used the roGFP transformants to examine a possible role of EGSH oxidation in mediating PCD in diatoms. Application of H2O2 induced cell death that was characterized by DNA degradation (detected by the TUNEL assay), externalization of phosphatidylserine (Annexin V staining) and compromised cell membrane (Sytox Green staining) (Figures 3a–i). Application of reduced GSH to cells treated with a lethal H2O2 dose (150 μM) rescued the cells from induction of cell death and led to full recovery of the EGSH in all examined organelles (Figure 3j, Supplementary Figure S2). Intriguingly, we identified a narrow time frame in which GSH addition rescued the cells from the lethal dose of H2O2. Addition of GSH 2 h or more after oxidation resulted in cell death 24 h after treatment (Figure 3j), despite the reduction of the organelle microenvironment. These results suggest an early critical phase in the subcellular response to redox perturbation induced by H2O2 and its subsequent regulation of cell fate. The inability of GSH to rescue cells after 2 h of oxidative stress could point at the induction of an irreversible signal transduction cascade that regulates cell death following perturbations in the EGSH.
Mitochondrial redox perturbation can predict cell fate under diverse environmental stress conditions
During algal bloom succession, diatom cells are subjected to diverse stress conditions at different phases of the bloom, from initiation to demise. As numerous diverse environmental stress conditions lead to increased ROS production, a key question is how specificity in signaling allows deciphering between multiple stressors and specific phenotypic responses (Mittler et al., 2004; Gadjev et al., 2006; Rosenwasser et al., 2013). To examine if environmental stresses differentially affect the subcellular EGSH, we monitored subcellular EGSH oxidation in response to common stress conditions that diatom cells may be exposed to in the aquatic environment (Figure 4, Supplementary Table S1). We observed different responses to nitrogen and iron limitation, both are major nutrients, which limit diatom blooms in the ocean (Boyd et al., 2007; Moore et al., 2013). Although an increase of 64% in the chloroplast roGFP oxidation level was detected in response to nitrogen limitation, none of the examined organelles were significantly oxidized following nine days of iron limitation (Figure 4, Supplementary Table S1 and see also Rosenwasser et al., 2014). Exposure of the cells to high light (700 μmol photons m−2 s−1) led to a significant oxidation of 11%, exclusively in the chloroplast (Figure 4 and Supplementary Table S1).
We further exposed P. tricornutum to physiological concentrations of BrCN, which was recently shown to act as an allelochemical in benthic diatoms (Vanelslander et al., 2012). We detected roGFP oxidation in all the examined subcellular compartments in response to 0.5 μM, but not 0.1 μM BrCN (Figure 4 and Supplementary Table S1). A cumulative, dose-dependent oxidation in the mitochondria was detected in response to the diatom-derived aldehyde 2E.4E/Z-decadienal (DD), an infochemical that is produced upon stress and is involved in chemical defense and intercellular signaling in diatoms (Ianora et al., 2004; Casotti et al., 2005; Vardi et al., 2006). Application of 50 and 100 μM DD led to maximal mitochondrial EGSH oxidation of 40% and 90%, respectively (Figure 4 and Supplementary Table S1). In contrast to the observed oxidation in the mitochondria, we detected only minor oxidation in the chloroplast, and slight dose-dependent oxidation in the nucleus in response to 5–100 μM DD. Maximal oxidation of <9% in the chloroplast and <13% in the nucleus were detected 3 h after treatment with 100 μM DD (Figure 4 and Supplementary Table S1). Taken together, these data indicate specific compartmentalized redox modification patterns in response to environmental stress conditions (Figure 4 and Supplementary Table S1). These organelle-specific oxidation patterns may be involved in sensing the cellular redox state under diverse environmental stress conditions and transmitting redox signals to respective targets.
Based on the observed interplay between early oxidation and subsequent cell death in response to H2O2 (Figures 2 and 3), we further linked early roGFP oxidation in specific organelles and cell death following diverse stress conditions. We plotted the maximal EGSH in each organelle versus the fraction of sytox-positive cells 24 h after exposure to the examined stress conditions (Figures 5a–c). Using the K-means clustering approach, we revealed two distinct clusters, one for stress conditions that kept reduced EGSH and did not lead to activation of cell death after 24 h (‘live’ cluster), and another cluster composed of stresses that led to oxidized EGSH pool and induction of cell death (‘dead’ cluster). To quantify how well EGSH serves as a predictor of cell fate, we analyzed the overlap between the histograms of the ‘live’ and ‘dead’ clusters as a function of EGSH (the X axis) in the various organelles (Figures 5d–f, see also detailed explanation in Supplementary materials and methods). The Minimal overlap between the two clusters (8%) was detected in the mitochondrial EGSH, which indicates a strong correlation between early oxidation of the mitochondrial EGSH and subsequent induction of cell death (Figure 5c). Intriguingly, we revealed a distinct life-death threshold of 23% oxidation, corresponding to –335 mV in mitochondrial EGSH, based on 200 samples obtained from application of various stress conditions (Supplementary Figure S3, Figures 5c, f and i). These results show a pivotal role for mitochondrial EGSH in cell fate determination and suggest that cell death can be precisely predicted based on the life-death mitochondrial EGSH threshold. We could not detect such a direct coupling between cell death and EGSH oxidation in the chloroplast or nucleus (Figures 5a and b, d and e, g and h). For example, nitrogen limitation led to high oxidation in the chloroplast without subsequent induction of cell death, while high DD concentrations led to induction of cell death with specific oxidation in the mitochondria, without oxidation of the chloroplast (Figure 4, and compare Figures 5g to 5i). Similarly, we did not observe a correlation between the EGSH in the nucleus and induction of cell death, as the lethal DD doses did not oxidize the nuclear EGSH, and some of the BrCN treatments (250 nM) oxidized the nuclear EGSH without induction of cell death (Figure 4, Figure 5h).
We further examined the role of early mitochondrial EGSH in mediating DD-induced cell death. The DD concentrations that led to early pronounced mitochondrial oxidation also led to extensive induction of cell death 24 h later (Figures 6a and b). Application of 1 mM GSH 30 min after treatment with the lethal concentration of 50 μM DD, suppressed both mitochondrial oxidation and induction of cell death (Figures 6b and c). Interestingly, this observed recovery was effective only within the 30-min time frame and at concentrations lower than 100 μM, corroborating our finding of a distinct critical phase of redox perturbation that determines the induction of downstream cell fate biochemical cascades.
Living within the chemo-physical gradient of light, nutrients and infochemicals, diatoms critically require mechanisms to rapidly respond to these diverse signals. Recent reports demonstrated the diatoms capabilities to perceive light, nutrients and infochemicals that are derived from trophic-level interactions (Falciatore et al., 2000; Vardi et al., 2006; Coesel et al., 2009; Huysman et al., 2013). Modulation in the cellular EGSH in response to environmental stress conditions has been suggested as a key signaling cascade enabling the transduction of information regarding the cellular redox homeostasis into redox-sensitive proteins in order to activate or adjust specific biological functions (Meyer, 2008; Foyer and Noctor, 2011). Recent studies on several organisms including diatoms, used proteomic approaches to map the cellular redox-regulated protein network, which may represent the capability of the cells to transmit specific redox signals into biological pathways (Leichert et al., 2008; Brandes et al., 2011; Kumsta et al., 2011; Rosenwasser et al., 2014). In this study, we used the redox-sensitive GFP sensor targeted to specific organelles in order to map the in vivo spatial and temporal redox patterns in the model diatom P. tricornutum under steady state and in response to environmental stresses. We provide compelling evidence that diatoms respond differentially to diverse stresses such as nutrient limitation, oxidative stress and chemical signals derived from biotic interactions, by organelle-specific oxidation patterns (Figure 5). Modulations in the redox homeostasis of specific cellular compartments following stress, can lead to post-translation modifications in the redox state of redox-sensitive proteins in this microenvironment and by that transmit a specific redox signal into relevant biological pathways. For example, specific oxidation of the chloroplast EGSH under high light and nitrogen starvation can reflect oxidative stress conditions that affect the redox state of chloroplast targeted redox-sensitive proteins to adjust their biological function in order to maintain the cellular homeostasis under these environmental constraints (Dietz and Pfannschmidt, 2011; Dangoor et al., 2012).
By examining the interplay between organelle-specific oxidation patterns and subsequent induction of cell death, we found a strong correlation between mitochondrial EGSH oxidation and cell death and defined a distinct life-death threshold of mitochondrial EGSH (–335 mV, 23% oxidation) (Figures 5f and i, Supplementary Figure S3). We further suggested a role for the oxidation state of mitochondrial EGSH in perception of DD-induced cell death. Mitochondria are recognized as having a central role in activation of ROS-dependent PCD in animals (Tait and Green, 2010; Rodriguez-Rocha et al., 2013) and oxidation of mitochondrial thioredoxin systems has been suggested as a common event in the initiation of PCD in animals and yeast (Zhang et al., 2004; Yin et al., 2012; Greetham et al., 2013). Nevertheless, very little is known about the molecular components involved in ROS perception and modulation and in execution of PCD in photosynthetic microorganisms. The absence of classical animal apoptotic components such as the Bcl-2 protein family and caspases suggests that although the mitochondria is a central mediator of PCD (Lam et al., 2001; Foyer and Noctor, 2003; Gadjev et al., 2008), the molecular pathways have diverged in animals and photosynthetic organisms. Moreover, even in animals, where the involvement of protein thiol status in mitochondrial permeability transition has been demonstrated (Kowaltowski et al., 2001), the proteins that mediate PCD by thiol oxidation are yet to be determined.
Taking into account the wide distribution of GSH as an antioxidant, possibly derived from a bacterial origin (Masip et al., 2006), it is possible that perturbation in EGSH is a conserved PCD signaling component (Koonin and Aravind, 2002; Bidle and Falkowski, 2004). Indeed, meta-analysis of plant seeds suggested EGSH as a universal marker for viability and cell death, whereby a shift of EGSH toward oxidation is correlated to the loss of seed viability (Kranner et al., 2006). Recently, the roGFP probe was used to identify a small fraction of susceptible neurons in a mouse model of Alzheimer’s disease, in which cell death was preceded by oxidation of EGSH (Xie et al., 2013). However, in these studies, the specific subcellular localization of the EGSH oxidation was not examined. Interestingly, a recently published study in mammalian cell culture found a relationship between mitochondrial roGFP oxidation in response to parkinsonian neurotoxins and later cell death (Rodriguez-Rocha et al., 2013). Taken together, these observations suggest that GSH has a central and evolutionary conserved role in cell death signaling. Although the precise threshold value described here for cell death activation can be alerted in diatom cells that are acclimated to diverse physiological conditions (for example, highlight, temperature and low DD), it is possible that a distinct shift of the mitochondrial EGSH (30 mV) from its steady-state condition can predict the induction of cell death. It is possible that the mechanisms underlying the induction of PCD as a result of mitochondrial EGSH oxidation are common in a wide range of organisms, the identification of a distinct EGSH threshold can help to identity unknown components of the mitochondrial machinery that perceive the redox signal and initiate the cell death biochemical cascade.
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We thank Robert Fluhr for critical comments on the manuscript. We thank James Remington from University of Oregon for providing the roGFP gene. This research was supported by the European Research Council (ERC) StG (INFOTROPHIC grant #280991) and the generous support of Edith and Nathan Goldenberg Career Development Chair to AV.
The authors declare no conflict of interest.
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van Creveld, S., Rosenwasser, S., Schatz, D. et al. Early perturbation in mitochondria redox homeostasis in response to environmental stress predicts cell fate in diatoms. ISME J 9, 385–395 (2015). https://doi.org/10.1038/ismej.2014.136
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