We present evidence for the directed formation of ice by planktonic communities dominated by filamentous diatoms sampled from the ice-covered Laurentian Great Lakes. We hypothesize that ice formation promotes attachment of these non-motile phytoplankton to overlying ice, thereby maintaining a favorable position for the diatoms in the photic zone. However, it is unclear whether the diatoms themselves are responsible for ice nucleation. Scanning electron microscopy revealed associations of bacterial epiphytes with the dominant diatoms of the phytoplankton assemblage, and bacteria isolated from the phytoplankton showed elevated temperatures of crystallization (Tc) as high as −3 °C. Ice nucleation-active bacteria were identified as belonging to the genus Pseudomonas, but we could not demonstrate that they were sufficiently abundant to incite the observed freezing. Regardless of the source of ice nucleation activity, the resulting production of frazil ice may provide a means for the diatoms to be recruited to the overlying lake ice, thereby increasing their fitness. Bacterial epiphytes are likewise expected to benefit from their association with the diatoms as recipients of organic carbon excreted by their hosts. This novel mechanism illuminates a previously undescribed stage of the life cycle of the meroplanktonic diatoms that bloom in Lake Erie and other Great Lakes during winter and offers a model relevant to aquatic ecosystems having seasonal ice cover around the world.
Winter presents a logistical obstacle to our understanding of temperate and boreal lake ecosystems. Reflecting this, lake ecosystem models frequently underestimate, or even dismiss, winter parameters. Addressing the void in our understanding of the winter ecosystem in Lake Erie, recent coordination with Coast Guard icebreaking programs has facilitated winter surveys of the lake since 2007 (Oyserman et al., 2012; Twiss et al., 2012). Conducted during times of expansive ice cover, these surveys have documented phytoplankton blooms dominated by physiologically robust, filamentous centric diatoms (Saxton et al., 2012; Twiss et al., 2012).
The physical processes involved in the development of diatom blooms in Lake Erie during winter are unclear. With potentially expansive ice cover across mid-high latitude lakes, light attenuation due to the ice and accumulated snow should act as a selective pressure in shaping adaptive mechanisms of phytoplankton found below the ice. In an ice-covered lake, it is imperative that phytoplankton occupy a position near the surface, which is a challenge for diatoms given that ice cover reduces wind-driven mixing and diatoms are non-motile plankton with high sinking rates. In ice-covered lakes, convective mixing is proposed as a mechanism to keep plankton suspended in the photic zone (Bengtsson, 1996; Kelley, 1997). Likewise, incorporation into ice cover, possibly via scavenging by frazil ice (Garrison et al., 1983), would offer a solution. Indeed, winter surveys of Lake Erie demonstrated the presence of viable diatoms in meltwater from lake ice (Twiss et al., 2012), consistent with a recent report documenting abundant Aulacoseira islandica colonizing vertical channels in ice sampled from the St Lawrence River, downstream from Lake Erie (Frenette et al., 2008).
During a recent winter survey of Lake Erie, ice formation was observed in samples of diatom-dominated plankton suspended in filtered lake water (FLW) and left in an insulated cooler on the ship’s deck overnight. Adjacent samples containing only filtered (<0.45 μm) lake water did not freeze. Biological ice nucleation associated with the diatom plankton offered a logical explanation for the discrepancy in whether samples froze.
Here, we document ice nucleation activity associated with filamentous diatoms sampled from the ice-covered Laurentian Great Lakes. Directed ice formation by diatoms promotes the formation of frazil ice, which provides buoyancy to the filaments and their subsequent attachment to overlying ice cover. This mechanism would increase fitness of diatoms by maintaining a favorable position in the photic zone, thereby facilitating their growth.
Materials and methods
Study site and sampling
Lake Erie was sampled on surveys aboard CCGS GRIFFON during February 2011–2012 and aboard R/V LAKE GUARDIAN during April 2010–2011. Sites in ice-covered western Lake Superior were sampled aboard USCGC ALDER in March 2011, whereas sampling in Lake Ontario was conducted aboard R/V LAKE GUARDIAN in April 2011. Physicochemical profiles of the water column were conducted as described in Supplementary Figure S1.
Plankton were collected by vertical net tows using a 154-μm mesh size plankton net deployed to depths of 10–15 m in water freed of ice cover by the vessel. Turbulence associated with icebreaker motion resulted in mixing of the ice-covered surface waters. High rates of sedimentation by diatoms collected at these locations in excess of 0.2 m h−1 guided the decision to collect samples integrated through these depths to ensure recovery of sufficient biomass for our analyses. Chlorophyll (Chl) a biomass was measured by fluorometry following extraction in 90% (v/v) acetone at −20 °C (Twiss et al., 2012). Water samples were collected using a 10-l Niskin bottle on a metered winch and by use of a submersible pump deployed 1 m below the surface. This water was filtered through a 0.2-μm filter and used for diluting phytoplankton collected by nets. During the 2012 survey, samples of ice were collected from Lake Erie. Individual pieces of ice (∼20 cm2) were passed through three rinses of filtered (<0.2 μm) lake water and placed in a sealed plastic bag to melt. The resulting meltwater was used for ice nucleation assays. Surface sediment was collected at several central basin sites using a ponar. Biofilm material adherent to 25-mm-diameter polycarbonate filters (0.2 μm) placed on the sediment surface was collected and resuspended in FLW before assay for ice nucleation.
Determination of temperature of crystallization of net plankton
Temperature of crystallization (Tc), was determined using ∼30 μl of sample in an 80 μl capillary tube (FisherBrand Micro-Hematocrit, Pittsburgh, PA, USA) to which a thermocouple was attached. Chl a biomass of phytoplankton samples loaded into microcapillary tubes ranged from 0.5–3 μg chl a ml−1. All samples collected in 2012 were diluted to 0.5 μg chl a ml−1 before freezing assay. Dilution trials (n=5) conducted in April 2010 and February 2012 demonstrated that samples diluted to at least 0.1 μg chl a ml−1 retained the same ice nucleation activity as an undiluted sample (Supplementary Figure S2). The capillary was placed in a foam-plugged glass test tube and suspended in a 5 °C cooling bath. A thermologger recorded the temperature at 30 s intervals. Following equilibration to 5 °C for 10 min, the temperature of the cooling bath was decreased at a controlled rate of 0.3 °C min−1. The Tc was recorded as the highest temperature at which ice crystals began to form, as indicated by the release of the latent heat of crystallization.
One-factor analysis of variances were conducted on samples collected during each field season, from each set of cultures, and during a heat denaturation experiment. Homogeneity of variance was tested for each analysis of variance by the Levene test with α=0.05, and normality was checked using normal Q–Q plots. A log(−Tc) transformation was required for homogeneity of variances in the denaturation experiment. Tukey’s HSD was used, with α=0.05, to compare Tc of samples and cultures against controls. All statistics were performed using the stats and car packages in R (version 2.12.2, www.r-project.org).
Scanning electron microscopy
Plankton were transferred to a glass vial and fixed by addition of glutaraldehyde to a final concentration of 2.5% (v/v) in 0.1 M sodium phosphate buffer (pH 7.2). Following two rinses with this buffer, samples were dehydrated through a graded series of acetone to 100%. Critical point drying was replaced by treatment with a solution of hexamethyl disilazane for 5 min and then air dried onto cover slips and coated with a 10-nm Au–Pd layer using a sputter coater (Hummer VI-A) before viewing with a Hitachi S2700 SEM (Hitachi High Technologies America, Inc., Dallas, TX, USA).
Isolation and characterization of heterotrophic bacteria
Plankton material collected by vertical net tows was streaked on Nutrient Broth agar plates (0.8% w/v nutrient broth (Difco, Becton Dickinson and Company, Franklin Lakes, NJ, USA), 1.2% w/v Bacto Agar (Difco)) and incubated at 4 °C in the dark for 72 h. Colonies from the plate were selected based on differences in colony morphology, and repeatedly subcultured at 4 °C so as to ensure purity of the final isolates. Isolates thus obtained were assayed for Tc as described above following standing culture growth in nutrient broth for 60–90 h at 4 °C. Enumeration of cell density was made by flow cytometry with densities ranging from 0.2—3.3 × 106 cells per ml used in the ice nucleation assay trials. Based on volumes loaded to microcapillary tubes for Tc assay, we estimate each capillary to have contained 0.6–9.9 × 104 cells.
Samples collected in 2012 were serially diluted with FLW before plating on cetrimide agar (Fluka Analytical, Sigma-Aldrich Corp., St Louis, MO, USA), a selective medium for isolating Pseudomonas spp. Plates were incubated at 20 °C for 2–5 days before enumeration.
Molecular characterization of bacterial strains
Bacterial SSU rDNA sequences were amplified by colony-PCR for all Lake Erie water sample isolates from 2010–2011 using the primers 8F (5′-AGAGTTTGATCMTGGCTCAG-3′) and 1492R (5′-GGYTACCTTGTTACGACTT-3′). The resulting PCR products were purified using a Qiagen PCR purification kit (Qiagen, Germantown, MD, USA). Purified fragments were sequenced by the DNA sequencing facility at the University of Chicago Cancer Research Center using standard methods. Ribosomal SSU DNA sequence data for the environmental bacterial isolates have been submitted to the GenBank database under accession numbers: JN201533–JN201573.
Thirty-four putative Pseudomonas isolate sequences were selected and combined with gamma-proteobacterial metagenomic sequences (Supplementary Figure S3), as well as the most similar NCBI sequences and published sequences of identified Pseudomonas taxa (Anzai et al., 2000), as determined from BLAST searches. A local multiple sequence alignment was performed with the MAFFT 6.833 command line version (Katoh et al., 2009; Katoh and Toh, 2010). The alignment file was manually evaluated with JalView (Waterhouse et al., 2009) and SeaView 4.3.2 (Gouy et al., 2010). Sequences shorter than 150 nucleotides were excluded from the final alignment. The alignment file was converted to the Nexus format with SeaView and subjected to phylogenetic analysis using PAUP 4.0b10 (Swofford, 1998). A total of 973 nucleotide positions were used for phylogenetic analysis. Neighbor-joining phylogenetic analysis was performed with 1000 bootstrap iterations.
We used sequences of nine known ina genes to design primers in an attempt to amplify the genes in our isolates. Sequences of four ina genes (inaZ, inaK, inaV and ice4) identified in Pseudomonas syringae were aligned using CLUSTAL-X software (www.clustal.org). The aligned sequences were then screened to identify regions of ∼20 bp that were conserved across all genes, but were not part of the repeat sequences characteristic of ina genes. Once identified, primers were designed for these sequences, and verified using the Primer-BLAST tool from NCBI. Similarly, primers were designed for other known ice-nucleating genes in P. fluoroscens (inaW), Xanthomonas campestris (inaX) and Erwinia spp. (iceE, inaU and inaA). Details of the sequences used and primers designed are listed in Supplementary Table S1.
We randomly selected 10 environmental isolates collected in 2011 that showed elevated Tc levels and assayed for the presence of ina genes. Ice-nucleating strains of P. syringae (ATCC 39254), P. fluorescens (CPBG 5 and RSG, isolated from the gut of Colorado potato beetle and Rana sylvatica, respectively) and P. putida (CPBG 8, CPBG 10, RSG) were used as positive controls. A non-ice-nucleating strain of P. fragi (ATCC 4973) was used as a negative control. Primers designed for the ina genes (Supplementary Table S1) were used in colony-PCR reactions with a PCR Master Mix (Promega Corporation, Madison, WI, USA) and a MJ Mini Thermal Cycler (Bio-Rad, Life Science Research, Hercules, CA, USA) under the following conditions: initial denaturation at 95 °C for 300 s, 45 cycles of 95 °C for 30 s (denaturation), 63 °C for 60 s (annealing), 68 °C for 90 s (extension) and a final extension at 68 °C for 300 s. PCR products were purified using a Qiagen PCR purification kit and sequenced at the University of Chicago Cancer Research Center. Sequences obtained were cross-referenced with sequences in the GenBank NCBI database to identify them.
Freezing column experiments
The approach of Garrison et al. (1989) with some modifications was used to investigate the incorporation of plankton into ice. To a 600-ml double-jacketed Pyrex beaker was added 450-ml FLW and 20 ml of plankton material collected from each of three central basin sites. Chl a biomass of fresh phytoplankton added to each column ranged from 70–90 μg chl a l−1. The double-jacketed beaker was connected to a 4 °C recirculating cooling bath containing propylene glycol. Following equilibration to 4 °C for 10 min, the temperature of the cooling bath was decreased at a rate of ∼0.2 °C min−1. Upon initial formation of frazil ice, the temperature of ice formation was noted and further cooling was stopped. Ice was separated from the liquid phase, the volume of each recorded and chl a biomass was determined from each phase. Assays were conducted in triplicate.
Controls differed in that the column was seeded with plankton that had been autoclaved for 20 min. Additional controls consisted of purified water (>18 MΩ cm) and filtered (<0.2 μm) lake water.
Results and Discussion
Ice nucleation in phytoplankton assemblages
We analyzed samples from winter and spring phytoplankton assemblages in Lake Erie between 2010 and 2012 for potential ice nucleation activity based on the Tc values of these samples. In samples subjected to a slow and controlled cooling process, the temperature at which ice formation is first detected is identified as the Tc of that sample. Mean Tc values for plankton from winter and spring assemblages were significantly higher than FLW or deionized water controls (Figure 1). Likewise, winter and spring assemblages from two other Great Lakes, Superior and Ontario, also showed elevated Tc values (Figure 1).
The ice nucleation activity was heat labile, consistent with it being derived from a biological protein source. The Tc of Lake Erie phytoplankton samples exposed to heat for 2 h decreased significantly compared with samples held at 4 °C (Supplementary Figure S4). Not all ice nucleation activity was lost as samples showed elevated Tc values compared with controls even after exposure to 45, 65 or 95 °C (Tukey’s HSD test, symbols bcd on Supplementary Figure S4, P<0.01).
Ice nucleation activity was not restricted to samples from the water column but was also measured in samples from ice melt and surface sediment collected during February 2012 (Figure 1). Although Lake Erie was nearly ice-free during this survey, a field of pancake ice (Supplementary Figure S5a) was encountered near the junction between western and central basins. Chl a biomass was concentrated >100-fold in the ice (470 μg l−1) compared with the underlying water. Likewise, sediment samples from central basin sites possessed a distinct brown-colored biofilm on the sediment surface (Supplementary Figure S5b) consisting of filamentous diatoms, which also possessed high ice-nucleating activity (Figure 1). Resting cells of diatoms have been identified from surficial sediments in the Great Lakes with Lake Erie meroplankton dominated by A. islandica and Stephanodiscus spp. (Lashaway and Carrick, 2010).
Whereas sea ice supports diverse (Mock and Thomas, 2005) and productive (Arrigo et al., 1997) assemblages of planktonic microbes in polar waters, only limited information is available on the ice cover habitat in freshwater ecosystems (Felip et al., 1995; Priscu et al., 1999; Bondarenko et al., 2006; Frenette et al., 2008). Diatoms are the most abundant phytoplankton in sea ice and have been implicated in ice-structuring activities that may promote their growth in ice (Raymond et al., 1994; Krembs et al., 2011). Extracellular polymeric substances produced by sea-ice microbes alter the structure of ice pores enhancing their habitability. Susceptibility of the activity to heat treatment as well as inhibition by glycosidase supports involvement of an ice-binding glycoprotein in the ice-structuring activity (Krembs et al., 2011). These proteins are thought to adsorb to the face of ice crystals, inhibiting their growth and further acting to structure the ice surface to promote light scattering for the associated algal cells (Raymond et al., 1994). Despite this ability to structure ice, the capacity to promote ice formation has not been specifically linked to sea-ice diatoms. Indeed, to our knowledge, ice nucleation activity associated with phytoplankton has been documented only twice before this report (Schnell, 1975; Schnell and Vali, 1975). In the former study, ice nucleation activity was not widespread with only phytoplankton collected from high latitude coastal waters of the North Atlantic Ocean testing positive. This was consistent with observations documenting bands of airborne ice nuclei at high latitudes between 40 and 55° (Bigg, 1973). Indeed, Schnell and Vali (1975) hypothesized that airborne ice nuclei might be biogenic in origin and derived from the ocean.
Bacterial epiphytes of diatoms can promote ice nucleation
Bacteria commonly colonize diatom cells (Amin et al., 2012). Scanning electron microscopy analysis of diatoms growing in association with ice cover showed a close association of flagellated rod-shaped bacteria with extracellular polymeric substances found on filaments of A. islandica (Figure 2) and S. binderanus (D’souza, 2012), both abundant in the winter assemblage (Twiss et al., 2012). Other diatom taxa were enumerated at low densities during winter and had few bacterial epiphytes (Supplementary Figure S6). Bacteria isolated from net plankton collected in 2010–2011 yielded 24 isolates exhibiting Tc values significantly higher than their growth medium (Figure 3), and similar to those from freshly collected diatom material (cf. Figure 1).
Among ice nucleation-active (INA) bacteria, ice nucleation activity resides on ina genes coding for ice nucleation proteins localized on the outer membrane of the bacterium (Orser et al., 1985). Unfortunately, our analysis was unable to definitively identify the INA component of the winter diatom community as evidence for known ina-like genes was not deduced from an ice metagenome from Lake Erie, nor did primers designed based on known ina sequences (Supplementary Table S1) amplify appropriate gene products from INA bacterial isolates (Supplementary Table S2). These same primers did amplify the ina genes in several control strains, including INA strains of P. syringae, P. fluorescens and P. putida (Supplementary Table S2).
Bacteria are the most active ice nucleators in the environment (Christner et al., 2008a), capable of catalyzing ice nucleation at temperatures as high as −2 °C (Lindow, 1983). INA bacteria were discovered in the 1970s and are all gamma-proteobacteria, including species in the genera Erwinia, Pseudomonas and Xanthomonas (Schnell and Vali, 1972; Maki et al., 1974; Lindow et al., 1978). A common life strategy among these strains is their association either as epiphytes on plants (Lindow, 1983) or commensals in gut microflora of ectotherms (Costanzo and Lee, 1996). Although INA bacteria have been isolated from lakes and streams (Morris et al., 2007; Morris et al., 2008), their presence in such non-host environmental reservoirs has been attributed to runoff and atmospheric deposition as rain or snow (Christner et al., 2008a; Christner et al., 2008b) consistent with their proposed role as biological ice nuclei in clouds (Christner et al., 2008a). However, a more recent analysis of genetic and phenotypic diversity identified clades of P. syringae distinct to water, arguing against the characterization of freshwater habitats as simple passive reservoirs in the life cycles of these bacteria (Morris et al., 2010).
Analysis of ribosomal RNA small subunit gene (rDNA SSU) sequences identified the INA bacterial isolates from our study as members of the genus Pseudomonas with high identity (>99%) to congeners P. chlororaphis, P. coronafaciens, P. fluorescens, P. fragi, P. syringae and P. viridiflava (Supplementary Figure S3). Metagenome samples acquired from lake ice contained sequences from pseudomonads closely related to the INA bacterial isolates (Supplementary Figure S3). Standard biochemical characterization identified the INA bacterial isolates from Lake Erie as phenotypically related to P. fluorescens (Supplementary Table S3). Ice nucleation activity is documented in P. syringae, P. fluorescens and P. putida (Lindow, 1983; Costanzo and Lee, 1996), whereas P. fragi is a known psychrophile, but for which ice nucleation activity has not been reported (Zdorovenko et al., 2004).
Environmental pseudomonads were enumerated from Lake Erie in February 2012. Consistent with a >100-fold partitioning of chl a biomass in the ice compared with the underlying water, Pseudomonas spp. were preferentially partitioned to the ice phase with log mean 6.125 logCFU l−1 (n=4) enumerated from melt water derived from surface ice. This was >3 orders of magnitude higher cell density (one-tailed t-test, p<0.0001) than Pseudomonas spp. enumerated in whole water (log mean=2.773 logCFU l−1 (n=11)). Pseudomonas spp. recovered from ice may include both diatom epiphytes and free-living bacteria, and their partitioning to ice is consistent with the active formation of frazil ice by biological ice nucleation as well as passive adherence of planktonic cells to frazil ice ascending to the surface as described previously (Garrison et al., 1983). By comparison, median abundance of P. syringae enumerated from the headwaters of rivers at 11 sites on three continents was 2.75 logCFU l−1 (Morris et al., 2010). Assuming that P. syringae comprises only a modest fraction of the total pseudomonads enumerated as part of the present study, the abundance reported here from lake water is comparatively low. Seasonality may have a role in the discrepancy as might the trophic status of the environment with rivers and coastal systems expected to support higher population densities of heterotrophs compared with open lake sites. Indeed, enumeration of Pseudomonas spp. made during spring 2012 showed an abundance of c 4 to 5.5 logCFU l−1 with the highest abundance reported from the mouth of the Maumee River in the lake’s western basin.
Despite the high abundance of Pseudomonas spp. recovered from lake ice and their potential role in ice formation, it is important to note that not every cell in a population of INA bacteria can serve as a potential ice nucleator. Depending on temperature and growth conditions, the ice nucleation frequency can change by >1 million fold (Hirano et al., 1985). Accounting for volumes used in Tc assays, we estimate each microcapillary containing phytoplankton collected in 2012 contained <100 CFU of Pseudomonas spp. Considering typical ice nucleation frequencies of INA pseudomonads, by most accounts, this is too few bacterial cells to account for the ice nucleation activity observed. However, we cannot discount the likely presence of viable but non-culturable pseudomonads in our study system as highlighted elsewhere (Ahern et al., 2007) leaving the identity of ‘who’ is responsible for the ice nucleation activity unresolved for now. Thus, while some of the viable pseudomonads recovered from net phytoplankton are shown to possess ice nucleation activity in culture, we cannot with certainty attribute the observed elevated Tc values of phytoplankton collected from Lake Erie to these bacteria. Further, regardless of their role in promoting ice nucleation, Pseudomonas spp. bacteria associated with the diatoms are expected to benefit from their partitioning to the ice phase. It is long recognized that dissolved organic carbon excreted by algae is exploited by heterotrophic bacteria (Cole et al., 1982) and provides up to 50% of the bacterial carbon requirement (Baines and Pace, 1991). In the case of epiphytic bacteria, all of the carbon required for growth can be provided by the photosynthetic host (Kirchman et al., 1984). Thus, for Pseudomonas spp., overwintering in association with actively growing diatoms is expected to increase fitness of the bacteria via enhanced nutrient acquisition.
Whereas previous studies strongly implicate bacteria as the INA component of phytoplankton (Schnell and Vali, 1975; Fall and Schnell, 1985) and meltwater samples from sea ice (Parker et al., 1985), we cannot eliminate the possibility that it is the diatoms, themselves, which are responsible for ice nucleation activity observed in plankton samples. Horizontal gene transfer has been proposed as a mechanism to account for the high similarity between ice nucleation protein sequences from diverse bacteria (Edwards et al., 1994). Further, recent evidence supports a role for the horizontal transfer of ice-binding protein genes from bacteria to sea-ice diatoms (Raymond and Kim, 2012), consistent with findings from recently sequenced diatom genomes showing a high rate of gene transfer between bacteria and diatoms (Bowler et al., 2008). Alternatively, perhaps diatoms possess an as yet unidentified mechanism to promote ice nucleation. Indeed, there are multiple reports of eukaryotes active in ice nucleation, including lichens (Kieft, 1988; Henderson-Begg et al., 2009), fungi (Pouleur et al., 1992), higher plants (Gross et al., 1988; Brush et al., 1994) and several species of Antarctic chlorophyte soil microalgae (Worland and Lukešová, 2000). In most cases, the eukaryotic ice nucleation activity exhibits differences to that of bacteria, including greater resistance to heat treatment (Kieft, 1988; Pouleur et al., 1992) thus drawing some parallels with results presented here on the heat lability of ice nucleation activity associated with net phytoplankton (Supplementary Figure S4).
Ice formation influenced by INA microbes
With the reduced radiant flux that accompanies winter in temperate and high latitudes coupled with expansive ice cover on lakes in these regions, reduced light availability is expected to have an important role in shaping adaptive mechanisms of phytoplankton located below the ice at this time. A few centimeters of snow accumulated on ice can result in high attenuation of photosynthetically active radiation (PAR) (Bolsenga and Vanderploeg, 1992).
As non-motile phytoplankton, diatoms rely mainly on physical mixing to remain suspended in the photic zone. As ice cover presents a barrier to wind-aided mixing, convective mixing has been proposed to allow diatoms to remain suspended near the water–ice interface in Lake Baikal (Kelley, 1997) that, like Lake Erie, experiences ice-associated blooms of Aulacoseira (Bondarenko et al., 2006).
During cold nights in winter, supercooled (<0 °C) water can be mixed to the sediments. When seeded with ice crystals from the air above, these supercooled conditions promote the formation of frazil ice and ultimately anchor ice in rivers and lakes (Martin, 1981) including the Laurentian Great Lakes (Kempema et al., 2001; Daly and Ettema, 2006). Much of the frazil and anchor ice formed in supercooled water ascends to the surface and can become incorporated into ice cover. Scavenging of suspended particulate matter, including diatoms by frazil crystals and sediment-scouring anchor ice, may thus provide a means of recruitment to the photic zone for phytoplankton (Garrison et al., 1983; Reimnitz et al., 1993). However, rather than relying solely on passive recruitment, we propose that the phytoplankton assemblage actively promotes the formation of frazil ice, providing cells with a buoyant ice raft to ascend to the photic zone (Figure 4). Recently, a similar envirotactic role for an ice-binding protein produced by the psychrophilic bacterium Marinomonas primoryensis was proposed, whereby ice binding serves to maintain the bacteria close to the surface in meromictic Antarctic lakes where oxygen is more abundant (Guo et al., 2012).
We tested our hypothesis using freezing columns seeded with plankton collected from Lake Erie. Phytoplankton biomass was preferentially partitioned to the ice phase in each assay with an average concentration factor (chl a in ice/water) of 12.2±3.2 (Figure 5). Plankton autoclaved before seeding the columns was also partitioned into the ice, although with a lower average concentration factor of 3.6±0.9 (one-tailed t-test, P<0.01) and similar to that reported from assays using sea-ice diatoms where partitioning was attributed to trapping and adherence by frazil ice (Garrison et al., 1989). Likewise, here, the modest concentration of heat-treated plankton in ice may be attributed to passive physical mechanisms, whereas the higher concentration of fresh plankton in newly formed ice is consistent with the active formation of frazil ice by biological ice nucleation thereby promoting the association of plankton with ice. Further, when ice formed in columns seeded with fresh plankton, its formation was rapid, occurring within seconds (Supplementary Video S1). This, we attribute to the greater abundance of active nucleation sites available in samples containing fresh phytoplankton.
Our results with freezing columns clearly demonstrate the enhanced partitioning of phytoplankton biomass to the ice phase associated with phytoplankton that are effective ice nucleators and is consistent with our analysis of lake ice, which likewise showed both elevated phytoplankton biomass and culturable Pseudomonas spp. cells compared with the water column. We cannot identify with certainty ‘who’ is responsible for the ice nucleation activity. Pseudomonad bacteria isolated from the diatoms are effective ice nucleators, yet low abundance of culturable Pseudomonas spp. in Tc assays of phytoplankton prevents us from unequivocally attributing the activity to the bacterial epiphytes.
Regardless of the source of ice nucleation activity, we propose that association with the ice facilitates growth of diatoms during winter owing to a more favorable light climate in this microenvironment. Whereas transmission of PAR is compromised in the presence of ice cover, the decline need not be precipitous. Indeed, light transmittance in February 2011 on a clear day at a central basin location, which was covered with plate ice having a thickness >20 cm, was shown to attenuate PAR by ∼60% thus providing a similar level of light transmittance as reported as sufficient to support a massive diatom-dominated bloom in the ice-covered Chukchi Sea (Arrigo et al., 2012). Whereas snow cover might compromise the ability of phototrophs to harvest light, an expansive, thick snowpack is uncommon on large lakes such as Erie. Rather, snow falling on the ice surface of large lakes is likely to accumulate in discrete drifts with the resulting snow-free ice exhibiting high transmittance of PAR (Bolsenga and Vanderploeg, 1992) thereby facilitating the growth of cold-adapted diatoms partitioned to the ice and ice–water boundary zone.
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We thank D Smith, M Saxton, B Oyserman, N Levis, K Rapin, R Edgar, P Morris, M Twiss, S Wilhelm, R Smith, C Binding, S Watson, H Carrick, W Woityra and R Bourbonniere for assistance with field surveys and analyses. M Lee provided bacterial reference strains. B Boese, C Turcotte and L-J Ma and the 454 Life Sciences Early Access Program supported the metagenome analysis. Lake surveys were made possible through the support of the captains and crews of the CCGS GRIFFON, R/V LAKE GUARDIAN, USCGC ALDER, USCGC NEAH BAY and Technical Operations personnel from Environment Canada (B Lalonde and T Breedon) and EPA-GLNPO. We acknowledge the input from two anonymous reviewers whose insights greatly improved the manuscript. This publication is a result of work that was supported in part, by the Ohio Sea Grant College Program (project # R/ER-081 to RMLM, SOR and GSB) from the National Oceanic and Atmospheric Administration, U.S. Department of Commerce. Support is also provided by the Ohio Board of Regents, The Ohio State University, Ohio State University Extension, and participating universities. The Ohio Sea Grant College Program is administered by The Ohio State University. Support was also provided by Lake Erie Protection Fund grants 398-11 and 430-12 (RMLM), and by NSF grants IOS-0840772 (REL), ANT-0837559 (REL) and OCE-1230735 (RMLM and GSB).
The authors declare no conflict of interest.
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D'souza, N., Kawarasaki, Y., Gantz, J. et al. Diatom assemblages promote ice formation in large lakes. ISME J 7, 1632–1640 (2013). https://doi.org/10.1038/ismej.2013.49
- ice nucleation
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