Table 1: Bacterial strains and plasmids

From: Cooperation and cheating in Pseudomonas aeruginosa: the roles of the las, rhl and pqs quorum-sensing systems

Lab strain or plasmidRelevant propertyReference or origin
Strains  
 PAO1P. aeruginosa wild-type (obtained from M Vasil and U Ochsner)(Holloway et al., 1979)
 PAO1 lasRPAO1 derivative; ΔlasR, unmarked in-frame deletion from amino acid 102 to 216This study
 PAO1 rhlRPAO1 derivative; ΔrhlR, unmarked in-frame deletion from amino acid 102 to 225This study
 PAO1 pqsRPAO1 derivative; ΔpqsR, unmarked in-frame deletion from amino acid 19 to 252This study
 PAO1 lasIPAO1 derivative; ΔlasI, unmarked in-frame deletion from amino acid 12 to 176This study
 PAO1 rhlIPAO1 derivative; ΔrhlI, unmarked in-frame deletion from amino acid 13 to 185This study
 PAO1 pqsAPAO1 derivative; ΔpqsA, unmarked in-frame deletion from amino acid 32 to 419This study
 PAO1 ΔpqsA CTX-lux::pqsAAQ biosensor strain; chromosomal deletion of the pqsA gene in PAO1 containing mini CTX-lux::pqsA(Diggle et al., 2007b)
   
Plasmids  
 pJN105araC-pBAD cloned in pBBR1 MCS-5; GmR(Newman and Fuqua, 1999)
 pJN105.pqsR-HpqsR coding region in pJN105This study
 pJN105.pqsR-NpqsR coding region and upstream regulatory region in pJN105 devoid of araC-pBADThis study
 pJN105.lasR-HlasR coding region in pJN105(Lee et al., 2006)
 pJN105.lasR-NlasR coding region and upstream regulatory region in pJN105 devoid of araC-pBADThis study
 pJN105.rhlR-HrhlR coding region in pJN105(Schuster and Greenberg, 2007)
 pJN105.rhlR-NrhlR coding region and upstream regulatory region in pJN105 devoid of araC-pBADThis study
 pEX18TcTcR; oriT, sacB, gene replacement vector with MCS from pUC18(Hoang et al., 1998)
 pEX18Tc.ΔpqsRpEX18Tc with the pqsR gene containing an in-frame deletion from residue 19 to 252This study
 pEX18Tc.ΔlasRpEX18Tc with the lasR gene containing an in-frame deletion from residue 102 to 216This study
 pEX18Tc.ΔrhlRpEX18Tc with the rhlR gene containing an in-frame deletion from residue 102 to 225This study
 pEX18Tc.ΔpqsApEX18Tc with the pqsA gene containing an in-frame deletion from residue 32 to 419This study
 pEX18Tc.ΔlasIpEX18Tc with the lasI gene containing an in-frame deletion from residue 12 to 176This study
 pEX18Tc.ΔrhlIpEX18Tc with the rhlI gene containing an in-frame deletion from residue 13 to 185This study
 pUC18T-mini-Tn7T-TpBroad host range mini-Tn7 vector with Tp resistance gene cassette(Choi and Schweizer, 2006)
 pUC18R6KT-mini- Tn7T-TetBroad host range mini-Tn7 vector with Tc resistance gene cassetteCourtesy of Herbert P Schweizer
  1. Abbreviations: AQ, alkyl quinolone; MCS, multiple cloning site; P. aeruginosa, Pseudomonas aeruginosa.