The photosynthetic pigments of phototrophic microorganisms cover different regions of the solar light spectrum. Utilization of the light spectrum can be interpreted in terms of classical niche theory, as the light spectrum offers opportunities for niche differentiation and allows coexistence of species absorbing different colors of light. However, which spectral niches are available for phototrophic microorganisms? Here, we show that the answer is hidden in the vibrations of the water molecule. Water molecules absorb light at specific wavebands that match the energy required for their stretching and bending vibrations. Although light absorption at these specific wavelengths appears only as subtle shoulders in the absorption spectrum of pure water, these subtle shoulders create large gaps in the underwater light spectrum due to the exponential nature of light attenuation. Model calculations show that the wavebands between these gaps define a series of distinct niches in the underwater light spectrum. Strikingly, these distinct spectral niches match the light absorption spectra of the major photosynthetic pigments on our planet. This suggests that vibrations of the water molecule have played a major role in the ecology and evolution of phototrophic microorganisms.
In the late 19th century, Professor Theodor W Engelmann was the first to demonstrate that phototrophic organisms utilize specific parts of the light spectrum. He produced a ‘living action spectrum’, by illuminating filaments of the green alga Spirogyra with a light spectrum created by a prism glass. This revealed that oxygen-dependent bacteria accumulated near those parts of the algal filaments illuminated with red and blue light, thus demonstrating that the pigment chlorophyll absorbs red and blue light for photosynthesis (Engelmann, 1882). One year later, in 1883, Engelmann discovered the utilization of infrared wavelengths by purple bacteria (Engelmann, 1883). Since then, many photosynthetic pigments have been identified, each with its own characteristic absorption spectrum (Pfennig, 1967; Falkowski and Raven, 1997; Des Marais, 2000; Xiong et al., 2000; Béjà et al., 2001; Falkowski et al., 2004). How can we explain the specific set of pigments that have evolved on planet Earth? Why is there not a single black pigment that absorbs all wavelengths?
Utilization of the light spectrum can be interpreted in terms of classical ecological theory. Light offers a spectrum of resources. According to ecological theory, niche differentiation along a resource spectrum reduces competition between species, and thereby promotes their coexistence (Gause, 1934; MacArthur and Levins, 1967; May and MacArthur, 1972; Rueffler et al., 2006). Darwin's finches on the Galápagos islands provide a famous example. Niche differentiation along a spectrum of different seed sizes allows a variety of finch species to coexist (Darwin, 1859; Lack, 1974; Grant and Grant, 2002). Likewise, differences in light absorption spectra of species result in niche differentiation along the light spectrum. Indeed, competition models and experiments have shown that red and green picocyanobacteria can coexist by absorbing different parts of the light spectrum (Stomp et al., 2004).
Niche differentiation along the light spectrum is probably a common phenomenon in aquatic ecosystems. For instance, a recent field survey confirmed that the relative abundances of red and green picocyanobacteria in lakes and seas are related to the underwater light color (Stomp et al., 2007). Red picocyanobacteria dominate in relatively clear waters, where green light penetrates the deepest, while green picocyanobacteria dominate in turbid waters, where red light penetrates the deepest. Coexistence of red and green picocyanobacteria is widespread in waters of intermediate coloration. Likewise, many other studies have revealed a close correspondence between the absorption spectra of phototrophic microorganisms and the prevailing underwater light spectrum (for example, Pierson et al., 1990; Wood et al., 1998; Béjà et al., 2001; Vila and Abella, 2001; Rocap et al., 2003; Kühl et al., 2005; Bouman et al., 2006; Sabehi et al., 2007).
Which spectral niches are available for phototrophic microorganisms? If our planet would offer a continuum of spectral niches, then one would expect a free distribution of light absorption spectra along this continuum (as in Figure 1a). Alternatively, it might be that environmental conditions constrain part of the resource spectrum, such that only a few distinct niches are available (Figure 1b). In this paper, we show that vibrations of the water molecule create gaps in the underwater light spectrum. As a result, not all wavebands are equally available for photosynthesis. This yields a series of distinct spectral niches for phototrophic microorganisms.
Vibrations of water molecules
Water molecules are never at rest. They rotate and vibrate. Vibrations of water molecules occur in three modes, including symmetric stretching (v1), asymmetric stretching (v3) and bending (v2) of the water molecule (Figure 2a; see also, for example, Braun and Smirnov, 1993; Pegau et al., 1997; Sogandares and Fry, 1997). The energy for these vibrations is obtained by absorption of radiation. The vibrations are most intense at wavelengths matching the specific energy requirements of these motions. These wavelengths can be recognized as peaks in the absorption spectrum of pure water (Figure 2b). Because the energy requirements for symmetric and asymmetric stretching are rather similar, their absorption peaks coalesce into a large absorption peak at around 3000 nm. The bending vibrations occur at a lower energy level, resulting in an absorption peak at around 6000 nm. Harmonics of these vibrations occur at higher energy levels (that is, shorter wavelengths) that double or triple the required energy. As a result, harmonics of the bending and stretching vibrations can be recognized as shoulders in the visible and near-infrared range of the absorption spectrum of water. For instance, the distinct shoulders in Figure 2c, at 449, 514, 605, 742 and 972 nm have been identified as the seventh, sixth, fifth, fourth and third harmonics, respectively, of the symmetrical and asymmetrical stretch vibration (Braun and Smirnov, 1993; Pegau et al., 1997; Sogandares and Fry, 1997). The shoulder at 1130 nm has also been identified as a third harmonics, composed of the combination of a symmetrical, asymmetrical and bending vibration (v1+v2+v3). We will argue, below, that these subtle shoulders in the absorption spectrum of pure water have a major effect on the underwater light spectrum.
The underwater light color
The underwater light spectrum of aquatic ecosystems depends on light absorption by pure water as well as by other components, including dissolved organic matter (known as ‘gilvin’ in the optics literature), inanimate particulate organic matter (known as ‘tripton’) and phytoplankton. More specifically, according to Lambert–Beer's law the underwater light spectrum can be calculated as follows (Sathyendranath and Platt, 1989; Kirk, 1994; Stomp et al., 2007):
where I(λ,z) is the light intensity of wavelength λ at depth z, Iin(λ) is the spectrum of the incident solar irradiance, KW(λ) is the absorption spectrum of pure water (Figures 2b and c), KGT(λ) is the absorption spectrum of gilvin and tripton and KPH(λ) is the absorption spectrum of the phytoplankton community.
The incident solar irradiance has essentially a white spectrum with a few small dips from 450 to 900 nm, and two large gaps in the infrared at 950 and 1120 nm. These dips in the incident solar spectrum are due to light absorption by oxygen and water molecules in the atmosphere (Kirk, 1994). Pure water mainly absorbs red and infrared light, with several distinct shoulders (Figure 2c). In contrast, gilvin and tripton absorb strongly in the blue region of the spectrum (Figures 3a–c). More specifically, the absorption spectrum of gilvin and tripton is a decreasing function of wavelength, which can be described by a smoothly declining exponential curve (Bricaud et al., 1981; Kirk, 1994):
where KGT(440) is the attenuation coefficient of gilvin and tripton at a reference wavelength of 440 nm, and S is the slope of the exponential decline. The attenuation coefficient KGT(440) is proportional to the concentration of gilvin and tripton.
In waters with low concentrations of gilvin and tripton and low phytoplankton concentrations, light absorption by pure water dominates (for example, Kirk, 1994; Morel et al., 2007). This applies, for instance, to the oligotrophic waters of the subtropical Pacific Ocean (Figure 3a). Here, red light is absorbed by water within the upper 10 m, whereas blue light penetrates much deeper (Figure 3d). Indeed, selective absorption of red light is responsible for the blue color of the oceans of our planet. In coastal waters, like the Baltic Sea, gilvin and tripton concentrations are higher, and their light absorption is often of a similar magnitude as light absorption by water itself (Figure 3b). As a result, green light penetrates the deepest (Figure 3e). In peat lakes, gilvin and tripton concentrations are extremely high, such that blue and green lights are rapidly absorbed (Figure 3c). As a result, red light penetrates the deepest (Figure 3f). Hence, with increasing gilvin and tripton concentrations, the underwater light color is shifted from the blue part towards the green and red part of the spectrum.
To investigate in further detail how gilvin concentrations affect the underwater light spectrum, we measured the underwater light spectrum in a variety of different aquatic ecosystems, ranging from blue waters of the Pacific Ocean to brown waters of very humic lakes. In addition, we searched the literature for light spectra measured in microbial mats, and found a beautiful spectrum from the murky microbial mats surrounding Rabbit Creek Spouter in Yellowstone National Park (Boomer et al., 2000). Figure 4 shows graphical plots of the underwater light spectra measured at the euphotic depth. The euphotic depth is here defined as the depth at which the irradiance over the entire photosynthetically available spectrum for aquatic microorganisms (400–1100 nm) equals 1% of the irradiance at the water surface. With increasing gilvin and tripton concentration, the underwater light color is shifted towards the red and even the infrared region of the spectrum in Lake Groote Moost, Lake Heelder Peel and the Rabbit Creek Spouter mat. Surprisingly, the data do not show a smooth shift in the underwater light spectrum, but reveal a striking landscape of peaks and valleys (Figure 4). Some underwater light spectra consist of a single peak, like the spectra of the Pacific Ocean and the Baltic Sea. Other underwater light spectra display several peaks and valleys. Moreover, the same peaks and valleys reoccur in different aquatic ecosystems. For instance, Lake IJsselmeer shows a similar peak at 560 nm as the Baltic Sea, and shares two peaks at 640 and 700 nm with Lake Groote Moost. Lake Groote Moost and Lake Heelder Peel share conspicuous peaks at both 700 and 800 nm, separated by a deep valley at 740–760 nm (Figure 4). The spectrum of the Rabbit Creek Spouter mat partly overlaps with Lake Heelder Peel, and extends further into the infrared, with a large dip at around 935 nm. If the absorption spectrum of gilvin and tripton is a smoothly decreasing function of wavelength (equation (2)), then why do underwater light spectra produce such a striking landscape of peaks and valleys?
Small shoulders, large gaps
The spectrum of the incident solar irradiance (Figure 5a) might offer a possible explanation for the striking landscape of peaks and valleys in the underwater light spectra of Figure 4. However, according to equation (1), peaks and valleys in the solar spectrum are transferred linearly in the underwater light spectrum. That is, the peaks and valleys in the solar spectrum are not amplified with depth. Therefore, only major gaps in the solar spectrum can be recognized in underwater light spectra. For example, the sharp dip in the solar light spectrum at 765 nm, due to atmospheric oxygen (Figure 5a), can be recognized in the underwater light spectra of Lake Groote Moost and Lake Heelder Peel. Similarly, the major gap in the solar light spectrum at 935 nm, due to atmospheric water, can be recognized in the irradiance spectrum of the Rabbit Creek Sprouter mat. The remainder of the solar spectrum in the range of 450–1100 nm is rather flat, however, and does not bear any resemblance with the measured underwater light spectra (compare Figures 4 and 5a).
The subtle shoulders in the light absorption spectrum of pure water (Figure 5b) may offer an alternative explanation for the striking landscape of peaks and valleys in Figure 4. According to equation (1), subtle shoulders in the light absorption spectrum of water are amplified with depth. That is, consider two wavelengths, λ1 and λ2. For simplicity, assume that there is a subtle difference in light absorption by water at these two wavelengths, while the incident irradiance and light attenuation by other components would be equal for λ1 and λ2. Now, according to equation (1), the light intensities at these two wavelengths will diverge exponentially with depth:
This shows that, due to the exponential nature of light absorption, subtle shoulders in the absorption spectrum of water create large gaps in the underwater light spectrum.
To investigate the latter hypothesis in further detail, we calculated the underwater light spectrum in the absence of phytoplankton. In this way, we obtained the available niches in the underwater light spectrum that can be exploited as a potential playfield for the ecology and evolution of phototrophic microorganisms. More specifically, we used equations (1) and (2) to calculate 100 different underwater light spectra at the euphotic depth. The absorption spectrum of pure water was taken from the literature (Hale and Querry, 1973; Segelstein, 1981; Pope and Fry, 1997). The slope S in equation (2) typically varies between 0.010 and 0.020 nm−1, and we here assumed a typical value of S=0.017 nm−1 (Kirk, 1994). The calculations were made for a wide range of different gilvin and tripton concentrations, with KGT(440) values from 0.003 m−1 in very clear ocean waters (Morel et al., 2007) to more than 5000 m−1 in extremely turbid systems representative for microbial mats in sediments (Kühl and Jørgensen, 1994). As a consequence, the euphotic depths ranged from more than 200 m in clear ocean water to only a few mm in turbid sediments and microbial mats.
Figure 5c shows an overlay of all 100 underwater light spectra thus calculated, which reveals a landscape of peaks and valleys quite similar to the measured underwater light spectra in Figure 4. Comparison of Figures 5a and c show that the dips in solar irradiance have some effect on the underwater light spectra, but this effect is relatively small. For instance, the dip caused by atmospheric oxygen at 765 nm (Figure 5a) creates a small secondary valley in the calculated underwater light spectra (Figure 5c). This small secondary valley was also visible in the measured light spectra of Lake Groote Moost and Lake Heelder Peel (Figure 4). In contrast, our calculations show that, consistent with equation (3), the subtle shoulders in the absorption spectrum of water create large gaps in the underwater light spectrum. The shoulder of the sixth harmonics in the absorption spectrum of water (Figure 5b) creates a large gap in the underwater light spectrum at ∼514 nm (Figure 5c). This gap separates the measured underwater light spectra of the Pacific Ocean and the Baltic Sea (Figure 4). Likewise, the shoulder at the fifth harmonics in the absorption spectrum of pure water (Figure 5b) creates a gap in the underwater light spectrum at ∼600 nm (Figure 5c), which separates the measured underwater light spectra of the Baltic Sea and Lake IJsselmeer from the peaks of Lake Groote Moost and Lake Heelderpeel (Figure 4). The next shoulder in the absorption spectrum of pure water, at the fourth harmonics (Figure 5b), creates a large gap in the underwater light spectrum at 740–760 nm (Figure 5c). This corresponds to the gap within the measured underwater light spectra of Lake Groote Moost and Lake Heelder Peel (Figure 4). Finally, at ∼950 nm, the combination of a deep trough in the incident solar irradiance caused by water molecules in the atmosphere (Figure 5a) and a large shoulder at the third harmonics of liquid water (Figure 5b) create a deep gap in the underwater light spectrum (Figure 5c). This gap can be clearly recognized in the irradiance spectra of microbial mats (Pierson et al., 1990; Boomer et al., 2000), as exemplified by the Rabbit Creek Spouter mat (Figure 4). In other words, this exercise shows that the underwater light spectrum does not present a homogeneous playfield for the ecology and evolution of phototrophic microorganisms. Instead, the underwater light spectrum offers a number of distinct niches at specific wavebands, separated by deep gaps created by the shoulders in the light absorption spectrum of the water molecule.
Filling the niches
Have phototrophic microorganisms adapted the absorption spectra of their pigments to these distinct niches in the underwater light spectrum? Three examples are provided in Figure 3. The phytoplankton community in the subtropical Pacific Ocean is dominated by picocyanobacteria of the genus Prochlorococcus (Chisholm et al., 1988; Letelier et al., 1993). Prochlorococcus effectively absorbs the available blue light with its pigments divinyl-chlorophyll a and b (Figure 3g). In the Baltic Sea, the phytoplankton community at the euphotic depth is dominated by red-colored picocyanobacteria of the Synechococcus group (Stomp et al., 2007), which effectively absorb the available green light with their pigment phycoerythrin (Figure 3h). In peat lakes, the phytoplankton community is often dominated by green-colored phytoplankton species, like green cyanobacteria and green algae, which absorb the available red light with pigments such as phycocyanin and chlorophylls a and b (Figure 3i). This is a first indication that the light absorption spectra of phytoplankton communities are often well tuned to their underwater light environment.
To extend our analysis to the full light spectrum available for photosynthesis, from 400 to 1100 nm, we gathered absorption spectra of 20 phototrophic species containing a wide variety of different pigments. The species belong to the green sulfur bacteria, purple sulfur bacteria, purple non-sulfur bacteria, cyanobacteria, green algae, diatoms, chrysophytes and red algae (Table 1). We measured the light absorption spectra of seven species with an Aminco DW-2000 double-beam spectrophotometer. Light absorption spectra of the other 13 species were obtained from the literature (Table 1). The absorption spectra of the individual species are shown in Figure 6. An overlay of all 20 absorption spectra is shown in Figure 5d. The match between the peaks and valleys in the underwater light spectrum and the peaks and valleys in the light absorption spectra of this rich variety of photosynthetic pigments is striking. That is, the harmonics in the light absorption spectrum of pure water (Figure 5b) create a series of distinct niches in the underwater light spectrum (Figure 5c), which are effectively captured by the light absorption spectra of the major groups of phototrophic microorganisms inhabiting our planet (Figure 5d).
Peaks in the underwater light spectrum at wavelengths below the sixth harmonics (<514 nm) are captured by chlorophyll a and b, divinyl-chlorophylls a and b and accessory carotenoids. The peak in the underwater light spectrum between the sixth and fifth harmonics (514–604 nm) is captured by the phycoerythrins of red algae and cyanobacteria. Peaks in the underwater light spectrum between the fifth and fourth harmonics (604–760 nm) are captured by phycocyanin, chlorophylls a, b and d, and bacteriochlorophyll e. Bacteriochlorophyll a in various phototrophic bacteria captures the peaks in the underwater light spectrum between the fourth and third harmonics (760–960 nm). Finally, bacteriochlorophyll b in the purple bacterium Blastochloris viridis (formerly known as Rhodopseudomonas viridis) harvests the light energy available at wavelengths between both third harmonics (960–1130 nm). Interestingly, the absorption peak of B. viridis seems to be shifted a bit towards shorter wavelengths compared to its spectral niche (compare Figures 5c and d). Perhaps our model calculations do not provide a very accurate description of spectral niches in microbial mats, where scattering of light can play an important role (Pierson et al., 1990). Furthermore, the infrared part of the light absorption spectrum of water is sensitive to temperature (Collins, 1925; Braun and Smirnov, 1993), which may shift this spectral niche to slightly shorter wavelengths at high temperatures. Also, the exact location of the light absorption peak of B. viridis is probably sensitive to temperature, and the range of measurements on lab cultures may not be exactly the same as in the bacterium's native environment (Kiang et al., 2007). It would be interesting to investigate these issues further. Perhaps other species containing bacteriochlorophyll b are capable to harvest light at even longer wavelengths, and therefore match this infrared niche more closely.
In this paper, we have developed the hypothesis that vibrations of the water molecule generate a series of distinct niches in the underwater light spectrum, which are effectively utilized by the different phototrophic microorganisms inhabiting our planet.
Our hypothesis implicitly assumes that the underwater light spectrum is an important selective factor for the ecology and evolution of phototrophic microorganisms. This hypothesis is supported by several lines of evidence. Many physiological studies have shown that light color affects photosynthesis and growth rates of phototrophic microorganisms, as has been demonstrated for, for example, green sulfur bacteria (Montesinos et al., 1983; Vila and Abella, 1994), cyanobacteria (Wyman and Fay, 1986; Hauschild et al., 1991; Callieri et al., 1996) and eukaryotic phytoplankton (Holdsworth, 1985; Glover et al., 1987). Furthermore, laboratory competition experiments have shown that light color can act as a selective factor. For instance, Parkin and Brock (1980) studied competition between green and purple sulfur bacteria isolated from the sulfide containing waters of three stratified lakes with different underwater light spectra. They observed that green sulfur bacteria became dominant in flasks exposed to red light, whereas purple sulfur bacteria became dominant in flasks exposed to green light. Likewise, Stomp et al. (2004) studied competition between red and green picocyanobacteria isolated from the Baltic Sea. They developed a competition model that predicts that red picocyanobacteria should become dominant in green light, green picocyanobacteria should become dominant in red light, whereas red and green picocyanobacteria can coexist in the full spectrum provided by white light. The results of their competition experiments were consistent with these predictions. These studies demonstrated that light color plays a decisive role in the species composition of phototrophic communities, at least in controlled laboratory experiments.
Numerous field studies have confirmed that the species composition of phototrophic microorganisms is related to the underwater light spectrum. For instance, Figure 3 shows that Prochlorococcus in the subtropical Pacific Ocean is well tuned to the prevailing blue light, red picocyanobacteria in the Baltic Sea are well tuned to the prevailing green light, and green cyanobacteria and green algae in turbid waters are well tuned to the prevailing red light. Recent work has shown that the relative abundances of red and green picocyanobacteria show a clear link with the underwater light spectrum across many ecosystems (Stomp et al., 2007). Likewise, the relative abundances of proteorhodopsin containing bacteria absorbing either blue or green light have been explained by prevailing spectral light conditions (Béjà et al., 2001; Sabehi et al., 2007). Similar observations have shown a good correspondence between the absorption spectra of phototrophic microorganisms and the underwater light spectrum in clear oceans (Ting et al., 2002; Rocap et al., 2003; Bouman et al., 2006), coastal waters (Olson et al., 1990; Wood et al., 1998; Katano et al., 2007), lakes (Pick, 1991; Vörös et al., 1998; Vila and Abella, 2001) and microbial mats (Pfennig, 1967; Pierson et al., 1990; Kühl et al., 2005). Thus, there is overwhelming evidence from theory, laboratory experiments and field data that the underwater light spectrum is a major determinant of the species composition of phototrophic communities.
The novel part of our hypothesis is that the underwater light spectrum does not offer a continuum of niches (as in Figure 1a), but consists of a series of distinct niches (as in Figure 1b) created by vibrations of the water molecule. This rather unexpected prediction is supported by the striking similarity between the calculated peaks and valleys in the underwater light spectra (Figure 5c) and the observed peaks and valleys in the absorption spectra of phototrophic microorganisms (Figure 5d). That is, the absorption spectra of the major photosynthetic pigments fit the available niches in the underwater light spectrum. Moreover, each spectral niche can be occupied by several pigments, with slightly different absorption peaks. For example, the niche between the sixth and fifth harmonics is occupied by two types of phycoerythrin, known as phycourobilin (PUB; peak at 494 nm) and phycoerythrobilin (PEB; peak at 545 nm) (Toledo et al., 1999). Likewise, several variants of bacteriochlorophyll a cover the niche between the fourth and third harmonics. This indicates that, within each spectral niche, absorption peaks of phototrophic organisms may diverge, possibly driven by the evolutionary process of adaptive radiation (Schluter, 2000; Rueffler et al., 2006).
Yet, this part of our hypothesis is clearly open for further testing. For instance, laboratory competition experiments could simulate light environments that deviate from the underwater light niches predicted by our model. As a first test, mixtures of phototrophic microorganisms could be exposed to wavebands that are less available in underwater light spectra. For example, wavelengths around 600 nm are relatively less available due to strong absorption by the fifth harmonics of water (Figure 5c), and currently few microorganisms have their absorption peak at 600 nm (Figure 6). If this waveband becomes the prevailing light color in a long-term laboratory selection experiment, will selection favor new mutants that shift their absorption peak to 600 nm?
Another interesting test could be based on selection experiments in artificial water that lacks the characteristic absorption peaks of normal water (H2O). In so-called heavy water (D2O), the hydrogen atoms are replaced by heavier deuterium atoms. As a consequence, molecular vibrations of D2O occur at other frequencies, and the harmonics of heavy water molecules are shifted to the far-red compared to H2O. Hence, the absorption spectrum of D2O is completely different from that of H2O (Tam and Patel, 1979; Braun and Smirnov, 1993). Our hypothesis therefore predicts that, in light-limited systems, selection experiments in D2O will lead to phototrophic communities with other light absorption spectra than selection experiments in H2O.
In conclusion, our findings point at a striking causal relationship between the stretching and bending vibrations of the water molecule, the underwater light spectra of aquatic ecosystems and the ecology and evolution of phototrophic microorganisms.
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We thank M Laamanen and DM Karl for the opportunity to join cruise Cyano-04 on the Baltic Sea and HOT cruise 174 on the Pacific Ocean, and the crew of research vessels Aranda and Kilo Moana for their great help during sampling. We also thank B Pex, H van Overzee, R Poutsma and students of the MSc program Limnology & Oceanography 2005 for their help in the Dutch lakes, and HJ Gons and JC Kromkamp for the underwater light spectrum of Lake IJsselmeer, and their help with the filterpad method. Special thanks to O Béjà and the anonymous referee for their helpful comments on the manuscript. The research of MS and JH was supported by the Earth and Life Sciences Foundation (ALW), which is subsidized by the Netherlands Organization for Scientific Research (NWO). LJS acknowledges support from the European Commission through the project MIRACLE (EVK3-CT-2002-00087).
Supplementary Information accompanies the paper on The ISME Journal website (http://www.nature.com/ismej)
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Stomp, M., Huisman, J., Stal, L. et al. Colorful niches of phototrophic microorganisms shaped by vibrations of the water molecule. ISME J 1, 271–282 (2007). https://doi.org/10.1038/ismej.2007.59
- microbial evolution
- molecular vibrations
- niche differentiation
- phytoplankton competition
- spectral irradiance
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