Histological stains for cementum. Serial sections of the 44 dpn mouse mandibular first molar were stained with (a–d) H&E, (e–h) TB and (i–l) AB-NFR. Visualization of AEFC and CIFC is emphasized in the accompanying text. A dotted line is used to highlight the circumference of the CIFC in panels (b), (f) and (j). Original magnifications (×50, ×100, ×200 and ×400) are labeled over the panels. AB-NFR, Alcian blue and nuclear fast red; AEFC, acellular extrinsic fiber cementum; CIFC, cellular intrinsic fiber cementum; dpn, days postnatal; H&E, hematoxylin and eosin; TB, toluidine blue.