Effects of caspase-1 inhibition on hepatic steatosis. LDLR−/−.Leiden mice were fed a high-fat diet for 21 weeks (HFD; n=15) or HFD+caspase-1 inhibitor Ac-YVAD-cmk (40 mg kg−1 daily) starting treatment after 9 weeks of HFD (HFD+YVAD; n=15). Low-fat diet (LFD)-fed mice (n=15) were included as a reference. (a) Representative photomicrographs of hematoxylin and eosin-stained liver sections. (b) Macrovesicular steatosis did not differ between groups. (c) HFD feeding induced pronounced microvesicular steatosis, which was reduced in HFD+YVAD. (d) HFD-induced hepatic triglycerides tended to be reduced in HFD+YVAD. All data are from the t=21 weeks time point and are mean±s.e.m. **P<0.01 compared with HFD.