Effects of caspase-1 inhibition on high-fat diet (HFD)-induced body weight, adiposity and adipose tissue inflammation. LDLR−/−.Leiden mice were fed a HFD for 21 weeks (n=15) or HFD+caspase-1 inhibitor Ac-YVAD-cmk (40 mg kg−1 daily) starting treatment after 9 weeks of HFD (HFD+YVAD; n=15). Low-fat diet (LFD)-fed mice (n=15) were included as a reference. (a) HFD feeding significantly induced body weight relative to LFD, which was not affected by caspase-1 inhibition. (b) Body fat percentage was induced by HFD and was not affected in HFD+YVAD mice. (c) HFD induced pronounced adipose tissue inflammation specifically in the epididymal depot, which tended to be reduced in HFD+YVAD mice. (d) Representative photomicrographs of HPS-stained epididymal adipose tissue sections. All data are from the t=21 weeks time point and are mean±s.e.m. *P<0.05, ***P<0.001 compared with HFD.