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Animal Models

Obesogenic phenotype of offspring of dams fed a high multivitamin diet is prevented by a post-weaning high multivitamin or high folate diet

International Journal of Obesity volume 37, pages 1177–1182 (2013)Cite this article

Subjects

Abstract

Background:

High multivitamin (10-fold AIN-93G, HV) diets fed during pregnancy to Wistar rats increase characteristics of metabolic syndrome in offspring when weaned to the recommended vitamin (RV) diet.

Objective:

To determine whether the effects of HV gestational diets on obesogenic phenotypes in the offspring arise as a consequence of altered hypothalamic control of feeding behavior and if their increased food intake could be prevented by feeding them HV or high folate (10-fold folate, HFol) diets.

Methods:

Male offspring of dams fed HV diet during pregnancy weaned to RV, HV or HFol diets were compared with those born to RV dams and weaned to RV diet for 29 weeks. Food intake over 72 h and body weight were measured bi-weekly and weekly, respectively. Glucose response to a glucose load was measured at 18 weeks post weaning. Hypothalamic gene expression of feeding-related neuropeptides including neuropeptide Y, pro-opiomelanocortin (POMC), insulin receptor, leptin receptor, brain-derived neurotrophic factor (BDNF), receptors for dopamine (DopaR1/2/5) and serotonin (SeroR1A/2A/2C), as well as global DNA methylation and brain and plasma folate concentrations were measured at 29 weeks post weaning.

Results:

HV or HFol pup diets increased brain and plasma folate concentrations and prevented the increase in food intake (5%, P=0.03), body weight (8%, P=0.0006) and glucose response to a glucose load (36%, P=0.02) found in those fed the RV diet. Expression of anorexigenic POMC (P=0.004) and BDNF (P=0.02) was higher, and DopaR1 was lower (P=0.06) in pups fed the HV diet. The HFol pup diet partially brought BDNF to the control level (P=0.02) and lowered SeroR2A (P=0.008). Expression of other genes was unaffected. Global DNA methylation was similar among the diet groups.

Conclusion:

The obesogenic phenotype in offspring from HV-fed dams is prevented by feeding HV or HFol pup diets, possibly due to post-weaning modulation of food intake regulatory mechanisms.

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Acknowledgements

We thank the Canadian Institute of Health Research, Institute of Nutrition, Metabolism and Diabetes, Reference MOP-93624 for funding this research. Clara E. Cho is supported by the Natural Sciences and Engineering Research Council of Canada Postgraduate Scholarship, Diana Sánchez-Hernández and Sandra A Reza- López by the ConsejoNacional de Ciencia y Tecnologia (Mexico) and Pedro S.P. Huot by the Ontario Graduate Scholarship.

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Authors and Affiliations

  1. Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada

    C E Cho, D Sánchez-Hernández, S A Reza-López, P S P Huot, Y-I Kim & G H Anderson

  2. Department of Medicine, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada

    Y-I Kim

  3. Keenan Research Center of the Li Ka Shing Knowledge Institute, St. Michael’s Hospital, Toronto, Ontario, Canada

    Y-I Kim

  4. Division of Gastroenterology, St. Michael’s Hospital, Toronto, Ontario, Canada

    Y-I Kim

  5. Department of Physiology, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada

    G H Anderson

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  1. C E Cho
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Correspondence to G H Anderson.

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Cho, C., Sánchez-Hernández, D., Reza-López, S. et al. Obesogenic phenotype of offspring of dams fed a high multivitamin diet is prevented by a post-weaning high multivitamin or high folate diet. Int J Obes 37, 1177–1182 (2013). https://doi.org/10.1038/ijo.2012.210

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