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Increased adipose tissue hypoxia and capacity for angiogenesis and inflammation in young diet-sensitive C57 mice compared with diet-resistant FVB mice

International Journal of Obesity volume 37, pages 853–860 (2013)Cite this article

Subjects

Abstract

Objective:

High-fat diets (HFDs) result in increased body weight. However, this is not uniform and determining the factors that make some animals or individual more susceptible to this diet-induced weight gain is a critical research question. The expansion of white adipose tissue (WAT) associated with weight gain requires high rates of angiogenesis to support the expanding tissue mass. We hypothesized that diet-induced obese (DIO) mice have a greater capacity for WAT angiogenesis and remodeling than diet-resistant (DR) mice at a young age, before age or DIO.

Design:

We measured body weight and body composition by nuclear magnetic resonance. We compared the expression of genes related to lipid metabolism, angiogenesis and inflammation by real-time, quantitative PCR and PCR arrays. WAT morphology and distribution of adipocyte size were analyzed. The level of hypoxia and vascular density was assessed by immunohistochemistry in WAT of young mice.

Results:

C57Bl/6 mice were DIO and FVB/N (FVB) mice DR after 8 weeks on a low-fat diet or HFD. However, C57Bl/6 mice had lower body weight, lower adiposity, smaller adipocytes and decreased leptin and lipogenic genes expression in adipose tissue than FVB mice at 9 weeks of age on a chow diet. Despite having smaller adipocytes, the level of hypoxia and the expression of pro-angiogenesis genes were higher in WAT of young C57Bl/6 mice than young FVB mice. In addition, expression of genes related to macrophages and their recruitment, and to proinflammatory cytokines, was significantly higher in WAT of young C57Bl/6 mice than young FVB mice.

Conclusion:

These data suggest that the potential for WAT remodeling in early period of growth is higher in C57Bl/6 mice as compared with FVB mice, and we hypothesize that it may contribute to the increased susceptibility to DIO of C57Bl/6 mice.

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Acknowledgements

This work was supported by National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) Grant DK093848 and Basic Science Research Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2012-0003537).

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Authors and Affiliations

  1. Department of Pharmacology, Korea University College of Medicine, Seoul, Republic of Korea

    D-H Kim & H-J Kim

  2. Department of Internal Medicine, University of Cincinnati, Cincinnati, OH, USA

    R Gutierrez-Aguilar & R J Seeley

  3. Department of Psychiatry, University of Cincinnati, Cincinnati, OH, USA

    S C Woods

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Correspondence to R J Seeley.

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Competing interests

Dr Seeley has served as a paid consultant for Novo Nordisk, Eli Lilly, Angiochem, Novartis and Ethicon Endo-Surgery; as a paid speaker for Novo Nordisk, Pfizer and Ethicon Endo-Surgery; and has received research support from Novo Nordisk, Pfizer, Mannkind, Ablaris and Ethicon Endo-Surgery.

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Kim, DH., Gutierrez-Aguilar, R., Kim, HJ. et al. Increased adipose tissue hypoxia and capacity for angiogenesis and inflammation in young diet-sensitive C57 mice compared with diet-resistant FVB mice. Int J Obes 37, 853–860 (2013). https://doi.org/10.1038/ijo.2012.141

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