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Cirsimarin, a potent antilipogenic flavonoid, decreases fat deposition in mice intra-abdominal adipose tissue

International Journal of Obesity volume 34, pages 1566–1575 (2010)Cite this article

Subjects

Abstract

Objective:

We previously reported that the flavonoid cirsimarin exerts in vitro a strong lipolytic activity on isolated adipocytes. This study was therefore designed to evaluate in vivo the effects of cirsimarin on white adipose tissue (WAT) accretion in mice.

Methods:

Male CD1 mice were injected daily with either vehicle (intraperitoneal (i.p.)) or cirsimarin (25 or 50 mg kg–1 per day, i.p.) for 18 days. Mice were killed and fat pads weighted. Epididymal fat pads were used for cellularity measurement. Effects of cirsimarin treatment on lipolysis and lipogenesis in WAT were assessed.

Results:

Mice treated with 25 or 50 mg kg–1 per day cirsimarin showed a decrease in retroperitoneal (−29 and −37% respectively, P<0.005) and epididymal (−25 and −28% respectively, P<0.005) fat pad weights compared with controls. This effect was restricted to intra-abdominal WAT as no difference was noticed for subcutaneous inguinal WAT. The decrease in intra-abdominal WAT accretion was due to a decrease in adipose cell diameter (−5 and −8% for 25 and 50 mg kg–1 per day cirsimarin, respectively) resulting in a 14 and 35% decrease in adipose cell volume while no change was noticed in total adipocyte number. Direct injection of cirsimarin (50 mg kg–1) to rats did not trigger lipolysis. In contrast, cirsimarin showed in vivo as well as in vitro a strong antilipogenic activity, which may be the critical aspect of its effects on fat accretion in mice. The inhibitory concentration 50% of cirsimarin on lipogenic activity in isolated adipocytes was found to be 1.28±0.04 μM. Cirsimarin given orally reduced intra-abdominal fat accretion in mice.

Conclusion:

Cirsimarin exerts potent antilipogenic effect and decreases adipose tissue deposition in mice. Cirsimarin could therefore be a potential candidate for the treatment of obesity.

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Acknowledgements

We gratefully acknowledge FE Demarne (Gattefossé SA, Saint Priest, France) for the kind gift of Cirsimarin. Bader Zarrouki and Nicolas Pillon are recipient for grants from the French ‘Ministère de l’Education Nationale, de la Recherche et de la Technologie’. This project was supported by a grant from INSA-Lyon (grant ‘BQR 2009’) to C Soulage and S Chambert.

Author information

Author notes

  1. B Zarrouki and N J Pillon: These authors contributed equally to this work.

Authors and Affiliations

  1. Université de Lyon, Lyon, France

    B Zarrouki, N J Pillon, E Kalbacher, H A Soula, G Nia N'Jomen, A Geloen & C O Soulage

  2. INSA-Lyon, RMND, Villeurbanne, France,

    B Zarrouki, N J Pillon, E Kalbacher, H A Soula, G Nia N'Jomen, A Geloen & C O Soulage

  3. INSERM U870, Oullins, France

    B Zarrouki, N J Pillon, E Kalbacher, H A Soula, G Nia N'Jomen, A Geloen & C O Soulage

  4. INRA U1235, Faculté Lyon Sud, Oullins, France,

    B Zarrouki, N J Pillon, E Kalbacher, H A Soula, G Nia N'Jomen, A Geloen & C O Soulage

  5. Hospices Civils de Lyon, Service de Néphrologie, Lyon, France

    E Kalbacher

  6. Laboratoire de Chimie Organique, ICBMS, Lyon, France,

    L Grand & S Chambert

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Correspondence to C O Soulage.

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Zarrouki, B., Pillon, N., Kalbacher, E. et al. Cirsimarin, a potent antilipogenic flavonoid, decreases fat deposition in mice intra-abdominal adipose tissue. Int J Obes 34, 1566–1575 (2010). https://doi.org/10.1038/ijo.2010.85

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