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Blood orange juice inhibits fat accumulation in mice



To analyze the effect of the juice obtained from two varieties of sweet orange (Citrus sinensis L. Osbeck), Moro (a blood orange) and Navelina (a blond orange), on fat accumulation in mice fed a standard or a high-fat diet (HFD).


Obesity was induced in male C57/Bl6 mice by feeding a HFD. Moro and Navelina juices were provided instead of water. The effect of an anthocyanin-enriched extract from Moro oranges or purified cyanidin-3-glucoside (C3G) was also analyzed. Body weight and food intake were measured regularly over a 12-week period. The adipose pads were weighted and analyzed histologically; total RNA was also isolated for microarray analysis.


Dietary supplementation of Moro juice, but not Navelina juice significantly reduced body weight gain and fat accumulation regardless of the increased energy intake because of sugar content. Furthermore, mice drinking Moro juice were resistant to HFD-induced obesity with no alterations in food intake. Only the anthocyanin extract, but not the purified C3G, slightly affected fat accumulation. High-throughput gene expression analysis of fat tissues confirmed that Moro juice could entirely rescue the high fat-induced transcriptional reprogramming.


Moro juice anti-obesity effect on fat accumulation cannot be explained only by its anthocyanin content. Our findings suggest that multiple components present in the Moro orange juice might act synergistically to inhibit fat accumulation.

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  1. Diet, nutrition and the prevention of chronic diseases. World Health Organ Tech Rep Ser 2003; 916: i–viii, 1–149, backcover.

  2. Welsh JA, Cogswell ME, Rogers S, Rockett H, Mei Z, Grummer-Strawn LM . Overweight among low-income preschool children associated with the consumption of sweet drinks: Missouri, 1999-2002. Pediatrics 2005; 115: e223–e229.

    Article  Google Scholar 

  3. Malik VS, Schulze MB, Hu FB . Intake of sugar-sweetened beverages and weight gain: a systematic review. Am J Clin Nutr 2006; 84: 274–288.

    Article  CAS  Google Scholar 

  4. McGinnis JM, Gootman JA, Kraak VI, Institute of Medicine (US). Committee on Food Marketing and the Diets of Children and Youth. Food Marketing to Children and Youth : Threat or Opportunity?. National Academies Press: Washington, DC, 2006, xx, 516, pp.

    Google Scholar 

  5. Vos MB, Kimmons JE, Gillespie C, Welsh J, Blanck HM . Dietary fructose consumption among US children and adults: the third national health and nutrition examination survey. Medscape J Med 2008; 10: 160.

    PubMed  PubMed Central  Google Scholar 

  6. West Europe Fruit Juice Market Research. Trends Analysis 2008. International Z: UK, 2008.

  7. Neves MF . The Orange juice distribution channels: some characteristic, opportunities and threats. Ita Food Bev Tech 1999; 18: 15–28.

    Google Scholar 

  8. Perez-Cacho PR, Rouseff RL . Fresh squeezed orange juice odor: a review. Crit Rev Food Sci Nutr 2008; 48: 681–695.

    Article  CAS  Google Scholar 

  9. Rampersaud GC . A comparison of nutrient density scores for 100% fruit juices. J Food Sci 2007; 72: S261–S266.

    Article  CAS  Google Scholar 

  10. Rapisada P, Intrigliolo F . Anthocyanins in Blood Oranges: Composition and Biological Activity Vol 5. Pandalai SG, 2001.

    Google Scholar 

  11. Rapisarda P, Tomaino A, Lo Cascio R, Bonina F, De Pasquale A, Saija A . Antioxidant effectiveness as influenced by phenolic content of fresh orange juices. J Agric Food Chem 1999; 47: 4718–4723.

    Article  CAS  Google Scholar 

  12. Reforgiato Recupero G . La propagazione dell’arancio Tarocco e del clementine Comune Vol 9 Italus Hortus, 2002.

    Google Scholar 

  13. Rapisarda P . Sample preparation for vitamin C analysis of pigmented orange juices. Ital J Food Sci 1996; 251–256.

  14. Postorino E . I flavanoni dei succhi di arancia italiani. Essenze Deriv Agrum 1999; 149–158.

  15. Rapisarda P, Carollo G, Fallico B, Tomaselli F, Maccarone E . Hydroxycinnamic acids as markers of Italian blood orange juices. J Agric Food Chem 1998; 46: 464–470.

    Article  CAS  Google Scholar 

  16. Acquaviva R, Russo A, Galvano F, Galvano G, Barcellona ML, Li Volti G et al. Cyanidin and cyanidin 3-O-beta-D -glucoside as DNA cleavage protectors and antioxidants. Cell Biol Toxicol 2003; 19: 243–252.

    Article  CAS  Google Scholar 

  17. Shih PH, Yeh CT, Yen GC . Anthocyanins induce the activation of phase II enzymes through the antioxidant response element pathway against oxidative stress-induced apoptosis. J Agric Food Chem 2007; 55: 9427–9435.

    Article  CAS  Google Scholar 

  18. Hou DX, Kai K, Li JJ, Lin S, Terahara N, Wakamatsu M et al. Anthocyanidins inhibit activator protein 1 activity and cell transformation: structure-activity relationship and molecular mechanisms. Carcinogenesis 2004; 25: 29–36.

    Article  CAS  Google Scholar 

  19. Xia M, Ling W, Zhu H, Ma J, Wang Q, Hou M et al. Anthocyanin attenuates CD40-mediated endothelial cell activation and apoptosis by inhibiting CD40-induced MAPK activation. Atherosclerosis 2009; 202: 41–47.

    Article  CAS  Google Scholar 

  20. Guo H, Ling W, Wang Q, Liu C, Hu Y, Xia M . Cyanidin 3-glucoside protects 3T3-L1 adipocytes against H2O2- or TNF-alpha-induced insulin resistance by inhibiting c-Jun NH2-terminal kinase activation. Biochem Pharmacol 2008; 75: 1393–1401.

    Article  CAS  Google Scholar 

  21. Berniakovich I, Trinei M, Stendardo M, Migliaccio E, Minucci S, Bernardi P et al. p66Shc-generated oxidative signal promotes fat accumulation. J Biol Chem 2008; 283: 34283–34293.

    Article  CAS  Google Scholar 

  22. Carpene C, Iffiu-Soltesz Z, Bour S, Prevot D, Valet P . Reduction of fat deposition by combined inhibition of monoamine oxidases and semicarbazide-sensitive amine oxidases in obese Zucker rats. Pharmacol Res 2007; 56: 522–530.

    Article  CAS  Google Scholar 

  23. Tsuda T, Horio F, Uchida K, Aoki H, Osawa T . Dietary cyanidin 3-O-beta-D-glucoside-rich purple corn color prevents obesity and ameliorates hyperglycemia in mice. J Nutr 2003; 133: 2125–2130.

    Article  CAS  Google Scholar 

  24. Jayaprakasam B, Olson LK, Schutzki RE, Tai MH, Nair MG . Amelioration of obesity and glucose intolerance in high-fat-fed C57BL/6 mice by anthocyanins and ursolic acid in Cornelian cherry (Cornus mas). J Agric Food Chem 2006; 54: 243–248.

    Article  CAS  Google Scholar 

  25. Tsuda T, Ueno Y, Yoshikawa T, Kojo H, Osawa T . Microarray profiling of gene expression in human adipocytes in response to anthocyanins. Biochem Pharmacol 2006; 71: 1184–1197.

    Article  CAS  Google Scholar 

  26. Tsuda T . Regulation of adipocyte function by anthocyanins; possibility of preventing the metabolic syndrome. J Agric Food Chem 2008; 56: 642–646.

    Article  CAS  Google Scholar 

  27. Rapisarda P, Fanella F, Maccarone E . Reliability of analytical methods for determining anthocyanins in blood orange juices. J Agric Food Chem 2000; 48: 2249–2252.

    Article  CAS  Google Scholar 

  28. Rouseff RL, Youtsey CO . Quantitative survey of narirutin, naringin, hesperidin and neohesperidin in Citrus. J Agric Food Chem 1987; 35: 1027–1030.

    Article  CAS  Google Scholar 

  29. Terpstra AH, Beynen AC, Everts H, Kocsis S, Katan MB, Zock PL . The decrease in body fat in mice fed conjugated linoleic acid is due to increases in energy expenditure and energy loss in the excreta. J Nutr 2002; 132: 940–945.

    Article  CAS  Google Scholar 

  30. Huang DW, Sherman BT, Lempicki RA . Systematic and integrative analysis of large gene lists using DAVID Bioinformatics Resources. Nature Protoc 2009; 4: 44–57.

    Article  CAS  Google Scholar 

  31. Dennis G Jr SB, Hosack DA, Yang J, Gao W, Lane HC, Lempicki RA . DAVID: Database for annotation, visualization, and integrated discovery. Genome Biol 2003; 4: P3.

    Article  Google Scholar 

  32. Guarnieri S, Riso P, Porrini M . Orange juice vs vitamin C: effect on hydrogen peroxide-induced DNA damage in mononuclear blood cells. Br J Nutr 2007; 97: 639–643.

    Article  CAS  Google Scholar 

  33. Arbo MD, Larentis ER, Linck VM, Aboy AL, Pimentel AL, Henriques AT et al. Concentrations of p-synephrine in fruits and leaves of Citrus species (Rutaceae) and the acute toxicity testing of Citrus aurantium extract and p-synephrine. Food Chem Toxicol 2008; 46: 2770–2775.

    Article  CAS  Google Scholar 

  34. Sale C, Harris RC, Delves S, Corbett J . Metabolic and physiological effects of ingesting extracts of bitter orange, green tea and guarana at rest and during treadmill walking in overweight males. Int J Obes (Lond) 2006; 30: 764–773.

    Article  CAS  Google Scholar 

  35. Bui LT, Nguyen DT, Ambrose PJ . Blood pressure and heart rate effects following a single dose of bitter orange. Ann Pharmacother 2006; 40: 53–57.

    Article  Google Scholar 

  36. Haller CA, Benowitz NL, Jacob III P . Hemodynamic effects of ephedra-free weight-loss supplements in humans. Am J Med 2005; 118: 998–1003.

    Article  Google Scholar 

  37. Szkudelska K, Nogowski L, Szkudelski T . Genistein affects lipogenesis and lipolysis in isolated rat adipocytes. J Steroid Biochem Mol Biol 2000; 75: 265–271.

    Article  CAS  Google Scholar 

  38. Pyo YH, Seong KS . Hypolipidemic effects of monascus-fermented soybean extracts in rats fed a high-fat and -cholesterol diet. J Agric Food Chem 2009; 57: 8617–8622.

    Article  CAS  Google Scholar 

  39. Shisheva A, Shechter Y . Quercetin selectively inhibits insulin receptor function in vitro and the bioresponses of insulin and insulinomimetic agents in rat adipocytes. Biochemistry 1992; 31: 8059–8063.

    Article  CAS  Google Scholar 

  40. Rayalam S, Della-Fera MA, Baile CA . Phytochemicals and regulation of the adipocyte life cycle. J Nutr Biochem 2008; 19: 717–726.

    Article  CAS  Google Scholar 

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We thank Elena Beltrami for many helpful discussions and Paola Dalton for the help in writing the paper. We thank also Ivan Toschi, Valentina and Nicoletta Cesari for the help in energy expenditure analysis and Simone Paolo Minardi for the assistance in the Affymetrix analysis. This work was supported by the EU FP6 FLORA project (FOOD-CT-01730) awarded to MG, CT, PGP, SM and GRR. This work was financially supported by EU FP6 FLORA project (FOOD-CT-01730).

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Correspondence to M Giorgio.

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Titta, L., Trinei, M., Stendardo, M. et al. Blood orange juice inhibits fat accumulation in mice. Int J Obes 34, 578–588 (2010).

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  • blood orange
  • orange juice
  • fat accumulation
  • anti-obesity
  • anthocyanins
  • cyanidin-3-glucoside.

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