Abstract
In an effort to identify neuronal repair mechanisms of the major pelvic ganglion (MPG), we evaluated changes in the expression of nestin, an intermediate filament protein and neural stem cell marker following cavernous nerve crush injury (CNI). We utilized two groups of Sprague Dawley rats: (i) sham and (ii) bilateral CNI. Erectile responses to cavernous nerve stimulation (CNS) were determined at 48 h in a subset of rats. The MPG was isolated and removed at 48 h after CNI, and nestin immunolocalization, protein levels and RNA expression were evaluated. At 48 h, erectile responses to CNS in CNI rats were substantially reduced (P<0.05; ∼70% decrease in intracavernous pressure/mean arterial pressure) compared with sham surgery controls. This coincided with a dramatic 10-fold increase (P<0.05) in nestin messenger RNA expression and protein levels in the MPG of rats with CNI. Immunoflourescence microscopy demonstrated that nestin upregulation after CNI occurred within the ganglion cell bodies and nerve fibers of the MPG. In conclusion, CNI induces nestin in the MPG. These data suggest that nestin may be involved in the regenerative process of the cavernous nerve following crush injury.
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Acknowledgements
The present study was funded in part from an AUA Foundation Astellas USA Foundation MD/PhD grant to TJB, K08 National Institute of Health Career Development Award to TJB, from the European Society of Sexual Medicine (ESSM) Grant for Medical Research to CG and a grant from the German Society of Andrology to CG.
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Kutlu, O., Ross, A., Schaeffer, E. et al. Increased expression of nestin in the major pelvic ganglion following cavernous nerve injury. Int J Impot Res 24, 84–90 (2012). https://doi.org/10.1038/ijir.2011.50
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DOI: https://doi.org/10.1038/ijir.2011.50
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