Ethanolic extract of seeds of Bryonia laciniosa Linn was administered orally to groups of male albino rats at the dose levels of 50, 100, and 150 mg kg−1 body weight per day for 28 days. The changes in sexual behaviour, reproductive organ weights, histology of testis and epididymis, epididymal sperm density, and androgenic hormone levels were evaluated. The sexual behaviour parameters studied such as mount frequency, intromission frequency, mount latency, intromission latency were significantly affected. Increase in body weight as well as weight of testis, prostate, seminal vesicle, and epididymis was noticed. Transverse sections of testis exhibited increased spermatogenesis and a significant increase in sperm count in epididymis. The fructose content of seminal vesicle was also increased. The extract treatment also brought a significant increase in serum testosterone and luteinizing hormone levels. The studies clearly reflect androgenic activity of the extract and its effects on hypothalamic pituitary gonadal axis.
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Vaidya L . Bhavprakash. Motilal Banarsidas: Varanasi, 1970.
Panda H . Handbook on Herbal Medicine. National Institute of Industrial Research: New Delhi, 2004.
Kirtikar KR, Basu BD . Indian Medicinal Plants. Periodical Expert Book Agency: Delhi, 1987.
Pullaiah T . Encyclopedia of World Medicinal Plants. Regency Publication: New Delhi, 2006.
Gowrikumar G, Mani VVS, Chandrasekhararao T, Kaimal TNB, Lakshminarayana G . Diplocyclos palmatus L: a new seed source of punicic acid. Lipids 1981; 16: 558–559.
Mosaddik MA, Ekramul Haque M, Abdur Rashid M . Goniothalamin from Bryonopsis laciniosa Linn (Cucurbiataceae). Biochem Syst Ecol 2000; 28: 1039–1040.
Singh V, Malviya T . A non-ionic glucomannan from the seeds of an indigenous medicinal plant: Bryonia lacinosa. Carbohydr Polym 2006; 64: 481–483.
Mosaddik MA, Haque ME . Cytotoxicity and antimicrobial activity of goniothalamin isolated from Bryonopsis laciniosa. Phytother Res 2003; 17: 1155–1157.
Singh V, Malviya T, Tripathi DN, Naraian U . An Escherichia coli antimicrobial effect of arabinoglucomannan from fruit of Bryonia lacinosa. Carbohydr Polym 2009; 75: 534–537.
Gupta M, Mazumdar UK, Sivakumar T, Vamsi ML, Karki SS, Sambathkumar R et al. Evaluation of anti-inflammatory activity of chloroform extract of Bryonia laciniosa in experimental animal models. Biol Pharm Bull 2003; 26: 1342–1344.
Sivakumar T, Perumal P, Kumar RS, Vamsi ML, Gomathi P, Mazumder UK et al. Evaluation of analgesic, antipyretic activity and toxicity study of Bryonia laciniosa in mice and rats. Am J Chin Med 2004; 32: 531–539.
Chauhan NS, Sharma V, Dixit VK . Effect of Asteracantha longifolia seeds on sexual behaviour of male rats. Nat Prod Res 2009; E-pub ahead of print 14 September 2009; doi: 10.1080/14786410802588493.
Chauhan NS, Rao CV, Dixit VK . Effect of Curculigo orchioides rhizomes on sexual behaviour of male rats. Fitoterapia 2007; 78: 530–534.
Saksena S, Dixit VK . Role of total alkaloids of Mucuna pruriens baker in spermatogenesis in albino rats. Indian J Nat Prod 1987; 2: 3–7.
Gonzales GF . Functional structure and ultrastructure of seminal vesicles. Arch Androl 1989; 22: 1–13.
Bennassi-Benelli A, Ferrari F, Pellegrini-Quarantotti B . Penile erection induced by apomorphine and N-n-propylapomorphine in rats. Arch Int de Pharmacodyn Ther 1979; 242: 242–247.
Islam MW, Tariq M, Ageel AM, Al-said MS, Al-Yhya AM . Effects of Saliva haematodes on sexual behaviour of male rats. J Ethanopharmacol 1991; 33: 67–72.
Vogel HG . Drug Discovery and Evalution Pharmacological Assays. Springer-Verlag: Berlin, Heidelberg, New York, 2002.
De Krester DM . The testis. In: Austin and Short (eds). A Hormonal Control of Reproduction. Cambridge University Press: Cambridge, 1987.
Yakubu MT, Akanji MA, Oladiji AT, Adesokan AA . Androgenic potentials of aqueous extract of Massularia acuminata (G. Don) Bullock ex Hoyl. stem in male Wistar rats. J Ethnopharmacol 2008; 118: 508–513.
Thakur M, Chauhan NS, Bhargava S, Dixit VK . A comparative study on aphrodisiac activity of some ayurvedic herbs in male albino rats. Arch Sex Behav 2009; 38: 1009–1015.
Zanoli P, Benelli A, Zavatti M, Rivasi M, Baraldi C, Baraldi M . Improved sexual behavior in male rats treated with a Chinese herbal extract: hormonal and neuronal implications. Asian J Androl 2008; 10: 937–945.
Chauhan NS, Dixit VK . Spermatogenic activity of rhizomes of Curculigo orchioides Gaertn in male rats. Int J Appl Res Nat Prod 2008; 1: 26–31.
One of the authors, NS Chauhan, thanks AICTE New Delhi for providing National Doctoral Fellowship.
The authors declare no conflict of interest.
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Chauhan, N., Dixit, V. Effects of Bryonia laciniosa seeds on sexual behaviour of male rats. Int J Impot Res 22, 190–195 (2010). https://doi.org/10.1038/ijir.2009.62
- Bryonia laciniosa
- sexual behaviour
- follicle stimulating hormone
- luteinizing hormone
- reproductive organ
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