The effects of taurine and magnesium (Mg) supplementation on nicotinamide adenine dinucleotide phosphate (NAD(P)H) oxidase in spontaneously hypertensive rats (SHRs). In the Control group, Wistar Kyoto (WKY) rats and SHRs (n=6) received a low-Mg (60 mg per 100 g) diet with high-salt drinking water (1% NaCl) for 4 weeks. In the Taurine group, SHRs (n=6) received a 3% taurine solution in 1% NaCl for 4 weeks. In the Mg group, SHRs (n=6) received a high-Mg (360 mg per 100 g) diet with 1% NaCl for 4 weeks. In the Taurine+Mg group, SHRs (n=6) received 3% taurine solution and a high-Mg diet with 1% NaCl for 4 weeks. The expression of mRNA of NAD(P)H oxidase components gp91phox (a), p22phox (b), and p47phox (c) in the renal cortex were determined by a real-time reverse transcription PCR. The data are expressed as the mean±s.e.m. *P<0.05 and **P<0.01 between the indicated columns.