We investigated the influence of angiotensin-converting enzyme inhibitor (ACEi) treatment and physical exercise on arterial pressure (AP) and heart rate variability (HRV) in volunteer patients with hypertension. A total of 54 sedentary volunteers were divided into three groups: normotensive (NT Group), hypertensive (HT Group) and HT volunteers treated with ACEi (ACEi Group). All volunteers underwent an aerobic physical-training protocol for 15 weeks. HRV was investigated using a spectral analysis of a time series of R–R interval (RRi) that was obtained in a supine position and during a tilt test. Physical training promoted a significant reduction in the mean arterial pressure of the HT group (113±3 vs. 106±1 mm Hg) and the ACEi group (104±2 vs. 98±2 mm Hg). Spectral analysis of RRi in the supine position before physical training demonstrated that the NT and ACEi groups had similar values at low frequency (LF; 0.04–0.15 Hz) and high frequency (HF; 0.15–0.5 Hz) oscillations. The HT group had an increase in LF oscillations in absolute and normalized units and a decrease in HF oscillations in normalized units compared with the other groups. The HT group had the lowest responses to the tilt test during LF oscillations in normalized units. Physical training improved the autonomic modulation of the heart rate in the supine position only in the HT group. Physical training promoted a similar increase in autonomic modulation responses in the tilt test in all groups. Our findings show that aerobic physical training improves cardiac autonomic modulation in HT volunteers independently of ACEi treatment.
Hypertension is highly prevalent in the adult population and represents one of the leading causes of morbidity and mortality worldwide.1, 2 This condition is directly related to the development of cardiovascular diseases, encephalic vascular accident and renal insufficiency.3 The risk factors for hypertension are associated with many other factors, including lifestyle.4 The excessive ingestion of salt and alcohol, obesity, smoking and sedentariness represent the main behavioral factors that contribute to the occurrence and development of hypertension.5
The treatment of hypertension consists mainly of a pharmacological approach, and the angiotensin-converting enzyme inhibitors (ACEis) are an important class of medications.6 ACEis reduce arterial pressure (AP) by decreasing the peripheral vascular resistance, and they reduce cardiac hypertrophy and the proliferation of vascular smooth muscle cells.7, 8, 9 Additionally, ACEis have an important role in cardiac autonomic control. Some studies have shown that ACEi treatment of hypertension in experimental animals and humans improves baroreflex sensitivity and cardiac autonomic modulation.10, 11, 12 However, these results are controversial.
Regular physical exercise has been also used for the treatment of hypertension as a co-adjuvant to pharmacological treatment and as a first intervention before prescribing of medication.13 Regular physical exercise reduces AP,14, 15 adjusts body composition,16, 17 increases aerobic capacity,15, 16 controls serum fat levels16 and improves cardiac autonomic control.18
The benefits of aerobic physical training on cardiac autonomic control have been well documented in experimental and clinical studies.19, 20, 21 Regular physical exercise promotes a tonic decrease in sympathetic autonomic control and an increase in vagal control.22, 23 However, the effects of physical exercise and ACEis, alone or in combination, on heart-rate variability and the capacity for autonomic balance rearrangement (for example, during the tilt test) have not been investigated in hypertensive (HT) patients. We hypothesized that the association of hypertension treatment with ACEis and physical training provides greater effects on cardiac autonomic modulation and enhances the reduction of arterial pressure compared with either treatment in isolation. Therefore, this study investigated the influence of physical aerobic training on arterial pressure and cardiac autonomic modulation in HT volunteers and the association between physical training and pharmacological treatment with an ACEi.
In all 54 non-smoking sedentary volunteers of both genders with a mean age of 44±2 years who presented no muscular-skeletal alterations that would impede regular physical exercise were studied. The volunteers were divided into three groups: normotensive volunteers with a mean arterial pressure (MAP) of 94±3 mm Hg (NT group; 9 women and 10 men), untreated volunteers with mild essential hypertension (MAP=113±3 mm Hg) (HT Group; 7 women and 10 men), and volunteers with mild essential hypertension (MAP=104±2 mm Hg) who were treated with an ACEi (Enalapril; 10–20 mg per day) for 10–15 weeks (ACEi group; 9 women and 9 men). The subjects in this latter group exhibited blood pressure levels that were similar to the HT group before treatment with Enalapril (MAP=115±4 mm Hg). All subjects were informed of the study, and they read and freely signed an informed consent form. The local research ethics committee approved this study.
All of the volunteers submitted to an aerobic physical training protocol that consisted of a 45-min treadmill exercise three times a week for 15 weeks with a heart rate intensity of ∼70–80% of heart rate reserve. Aerobic capacity was re-assessed at the end of the 15-week training period.
Laboratory tests and anthropometric measurements
Serum levels of glycemia, triglycerides, total cholesterol and cholesterol fractions were measured before and after the 15-week physical training. The volunteers were instructed to fast for 12 h, avoid alcoholic beverage for 48 h before the laboratory tests, engage in no physical activity and maintain their habitual diet. The left ventricular mass index (LVM index) was calculated from the relationship between the left ventricular mass (g) from the echocardiogram and body surface (m2), which was calculated using the following relationship: (weight (kg)+height (cm)–60)/100. Body weight was measured to an accuracy of 0.1 kg, and the body mass index was computed as body weight/height2. Tetrapolar body electrical bio-impedance determined the percent fat mass.
Analysis of heart rate variability (spectral analysis)
The volunteers were instructed to avoid alcohol and caffeine, practice no physical activity and maintain their habitual diet for 48 h before laboratory exams. The spectral analysis of heart rate variability (HRV) was recorded between 0900 and 1000 hours according to the following protocol: after remaining in a supine rest position on inclinable bed for 20 min, the volunteers were passively placed in an inclined position (75° angle) for an additional 20 min. HRV for supine and inclined positions (that is, the tilt test) was recorded using an electrocardiogram (AD Instruments, Sydney, Australia), and a time series of RR interval (RRi) was obtained. This RRi time series was divided into continuous segments of 200 beats and overlapped with the previous series of 100 beats. The mean and variance of each segment were calculated, and these values were submitted to an auto-regressive spectral analysis as described previously.24, 25, 26 The oscillatory components in stationary segments (beat-to-beat RRi) were calculated based on the Levinson–Durbin recursion in accordance with Akaike's criteria.24 This method allows for the central frequency and influence of each relevant oscillatory component in the RRi series to be automatically quantified. The oscillatory components were classified as low frequency (LF; 0.04–0.15 Hz) or high frequency (HF; 0.15–0.5 Hz). The power of these components on RRi variability was calculated as the variability of LF and HF in percent values, considering the total power and following the subtraction of the very LF (<0.04 Hz), and the power was expressed in normalized units. This normalization procedure minimizes the effect on the absolute values of LF and HF variability that result from the change in total power.24, 25, 26 Finally, the LF/HF rate was calculated to establish an index of autonomic modulation.
Data are presented as the mean±the s.e. of the mean. Comparisons between the mean values in the three groups were performed using a one-way analysis of variance followed by Tukey's post-hoc test. Comparisons between the two mean values of the same variable before and after treatment within the same group were performed using the Student's t-test for paired samples. Comparisons between the various mean values within the same group were performed using a one-way analysis of variance followed by either Bonferroni's post-hoc test to compare the variable before and during the treatment or Tukey's test for comparisons between groups. Differences were defined as statistically significant at P<0.05.
Table 1 lists all of the characteristics of the subjects, metabolic values, hemodynamic values and LVM indexes before and after the 15-week aerobic physical training. No significant differences in age, height, initial and final weight, body mass index, percent body fat and metabolic values were observed between the groups. However, physical training increased the VO2peak in all groups. Basal HR decreased at the end of the protocol and was similar before and after physical training in all groups. AP was different in all groups before physical training. The HT group had the highest AP values, and the NT group had the lowest systolic, diastolic and mean values. All variables were reduced after physical training compared with baseline values in the HT group, but remained higher than the other groups. At the end of the protocol, the values for diastolic and MAP were similar between the ACEi and NT groups. All groups demonstrated different LVM values before physical training; the NT group presented the lowest values, and the HT group displayed the highest values. Physical training did not alter the LVM index in the NT group, but a reduction in the LVM index was achieved in the other groups. Physical training had a significant impact in the HT group, whose values were higher than the trained NT group.
Spectral analysis of HRV (supine rest position)
The HT group displayed an increase in both total variance and LF oscillations (absolute and normalized units) and a decrease in HF oscillations (normalized units) compared with the other groups (Table 2). Moreover, this group also demonstrated a significant increase in the LF/HF ratio. Physical training did not affect HRV in the supine rest position in the NT group. However, the ACEi group presented a significant reduction in the total variance (1842±281 vs. 1314±104 ms2) and HF oscillations in absolute units (328±67 vs. 213±41 ms2). In addition, physical training significantly improved HRV in the HT group, which was characterized by a reduction in LF oscillations in absolute (774±104 vs. 371±50 ms2) and normalized (68±3 vs. 56±4%) units, an increase in HF oscillations in normalized units (32±3 vs. 44±4%) and a reduction in the LF/HF ratio (2.5±0.4 vs. 1.3±0.2).
Spectral analysis of HRV (Tilt test)
Figure 1 presents representative spectra of RRi for all groups in the supine position and during the tilt test before and after physical training. Table 3 shows the results of the tilt test in volunteers before the 15-week physical training protocol (sedentary). The autonomic modulatory responses to the tilt test were not different between the NT and ACEi groups. In contrast, the HT group exhibited a greater reduction in total variance, a smaller increase in LF oscillations in percent values (Figure 2a), and an increase in the LF/HF ratio. After physical training (Table 3 and Figure 2), all groups demonstrated improved cardiac modulatory responses to the tilt test with no significant differences between groups. However, a smaller reduction in total variance was observed in the ACEi group.
This study demonstrated that aerobic physical training may enhance autonomic modulatory responses to the tilt test independently of the presence of hypertension or pharmacological treatment with an ACEi. Additionally, our study also demonstrated that aerobic physical training reduced arterial pressure in untreated HT volunteers and further reduced AP values in association with Enalapril compared with the use of either treatment alone.
Arterial hypertension is often accompanied by an impairment of cardiovascular autonomic control, which is characterized by an increase in sympathetic autonomic activity and a decrease in vagal autonomic activity assessed by different approaches of peripheral sympathetic nervous activity, such as baroreflex sensitivity and HRV analysis.21, 27, 28, 29 Many studies have demonstrated that HT volunteers exhibit a reduction in HRV that is associated with a decrease in vagal autonomic modulation and an increase in sympathetic modulation.30, 31, 32
Our study demonstrated that untreated HT volunteers presented an increase in HVR that was characterized by an increase in total variance in the RRi compared with NT and HT volunteers treated with Enalapril. Although this increase appears paradoxical, it was the result of an increase in sympathetic autonomic modulation because the LF oscillations in these volunteers were notably high. A reduction in HF oscillations was also observed in this group, which further contributed to the increase in LF/HF rate and indicated a greater predominance of sympathetic activity in the autonomic balance.
HRV can be evaluated in different situations, and the tilt test assesses autonomic balance rearrangement during postural stimuli.33, 34 In our study, the tilt test revealed that untreated HT volunteers had greater increases in the absolute values of LF oscillations but low responses in percent values. However, the percent values of the HF band were similar to other groups despite the lower absolute values. This finding suggests that the impaired cardiac autonomic control is mainly the result of the sympathetic autonomic component.31, 32, 33, 34, 35
Subjects in the ACEi group exhibited a significant reduction in arterial pressure compared with the HT groups and no difference in cardiac autonomic modulation compared with NT volunteers during the tilt test. This observation suggests that treatment with Enalapril re-established the cardiac autonomic modulation that was impaired by hypertension. Indeed, ACEis and AT1 receptor antagonists promote an improvement in autonomic function in different contexts, including baroreflex sensitivity evaluation and HRV analysis.11, 28, 30, 36, 37 The mechanisms that underlie ACEi improvement of HRV modulation have not been elucidated, but ACEis and/or AT1-receptor antagonists primarily affect neural sites that are involved in the cardiovascular regulation, such as the solitary tract nucleus, rostral ventrolateral medulla, paraventricular nucleus and area postrema. However, the identification of the exact mechanism of action has been hindered by the complex interaction of the sites involved in cardiovascular control.30, 38, 39, 40
Similar to Enalapril, physical training also reduces arterial pressure. The combination of Enalapril and physical training enhanced the reduction of AP, which reached values that were similar to the NT volunteers. The effect of physical exercise in HT patients with mild-to-moderate arterial hypertension has been described previously.41 Our results corroborate earlier studies and suggest that pharmacological therapy with physical exercise promotes better outcomes.
Physical training also promoted significant changes in HRV despite a more intensive and independent activity on angiotensin II blockade. Our results demonstrated that aerobic physical training improved the autonomic modulatory responses of HRV in all groups in addition to the re-establishment of the modulatory parameters in untreated hypertension volunteers, whose values were similar to those of NT volunteers. Therefore, the improvement in spectral parameters during the tilt test was similar between groups. Interestingly, aerobic physical training did not alter basal cardiac autonomic modulation in NT or HT volunteers treated with Enalapril. This result emphasizes the importance of the evaluation of cardiac autonomic modulation in different situations (for example, using the tilt test) to provide important elements of the capacity for autonomic balance rearrangement during therapeutic interventions.
Our findings support the importance of physical training for therapeutic and preventive purposes in the treatment of hypertension itself and account for important alterations in cardiac autonomic adjustments.18, 19 Physical exercise may promote adjustments in cardiovascular control sites, such as the hypothalamus, solitary tract nucleus and the rostral ventrolateral medulla. These adaptations may occur via neural remodeling or endogenous factors, such as nitric oxide.20, 42, 43, 44, 45, 46 These adaptations would promote a decrease in sympathetic autonomic influence and an increase in the participation of the vagal autonomic component in cardiac control to improve the autonomic balance. Additionally, physical exercise may also exacerbate the influence of angiotensin II on the modulation activity in specific neural sites,45 but further investigation is required.
Some limitations of our study should be addressed. Firstly, AP and HR were not consecutively assessed in all groups before treatment with Enalapril in the ACEi group. Secondly, a follow-up study of HRV during this same interval would have provided information on autonomic modulation in the ACEi group before treatment and allowed for a comparison of values from the HR group. Further longitudinal studies should be performed using the parameters of the present study to investigate the effects of other anti-HT drugs and physical training regimens.
In conclusion, our findings demonstrate that physical exercise is important for therapeutic and preventive purposes and serves as a co-adjuvant in the treatment of hypertension. This approach promotes cardiac autonomic modulation in HT volunteers independently of treatment with ACEis.
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This study was supported by FAPESP (2008/07561-0).
The authors declare no conflict of interest.
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