Effects of Nattokinase on Blood Pressure: A Randomized, Controlled Trial

Abstract

The objective of this study was to examine the effects of nattokinase supplementation on blood pressure in subjects with pre-hypertension or stage 1 hypertension. In a randomized, double-blind, placebo-controlled trial, 86 participants ranging from 20 to 80 years of age with an initial untreated systolic blood pressure (SBP) of 130 to 159 mmHg received nattokinase (2,000 FU/capsule) or a placebo capsule for 8 weeks. Seventy-three subjects completed the protocol. Compared with the control group, the net changes in SBP and diastolic blood pressure (DBP) were −5.55 mmHg (95% confidence interval [CI], −10.5 to −0.57 mmHg; p <0.05) and −2.84 mmHg (CI, −5.33 to −0.33 mmHg; p >0.05), respectively, after the 8-week intervention. The corresponding net change in renin activity was −1.17 ng/mL/h for the nattokinase group compared with the control group (p <0.05). In conclusion, nattokinase supplementation resulted in a reduction in SBP and DBP. These findings suggest that increased intake of nattokinase may play an important role in preventing and treating hypertension.

References

  1. 1

    Kearney PM, Whelton M, Reynolds K, Muntner P, Whelton PK, He J : Global burden of hypertension: analysis of worldwide data. Lancet 2005; 365 ( 9455): 217–223.

    Article  Google Scholar 

  2. 2

    Chobanian AV, Bakris GL, Black HR, et al; National Heart, Lung, and Blood Institute Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure; National High Blood Pressure Education Program Coordinating Committee: The Seventh Report of the Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure: the JNC 7 report. JAMA 2003; 289: 2560–2572.

    CAS  Article  Google Scholar 

  3. 3

    Gu D, Reynolds K, Wu X, et al, InterASIA Collaborative Group: The International Collaborative Study of Cardiovascular Disease in ASIA. Prevalence, awareness, treatment, and control of hypertension in china. Hypertension 2002; 40: 920–927.

    CAS  Article  Google Scholar 

  4. 4

    Choi KM, Park HS, Han JH, et al: Prevalence of prehypertension and hypertension in a Korean population: Korean National Health and Nutrition Survey 2001. J Hypertens 2006; 24: 1515–1521.

    CAS  Article  Google Scholar 

  5. 5

    Whelton PK, He J, Appel LJ, et al; National High Blood Pressure Education Program Coordinating Committee: Primary prevention of hypertension: clinical and public health advisory from The National High Blood Pressure Education Program. JAMA 2002; 288: 1882–1888.

    Article  Google Scholar 

  6. 6

    Neaton JD, Grimm RH Jr, Prineas RJ, et al: Treatment of Mild Hypertension Study. Final results. Treatment of Mild Hypertension Study Research Group. JAMA 1993; 270: 713–724.

    CAS  Article  Google Scholar 

  7. 7

    Iseki K, Kimura Y, Wakugami K, et al: Comparison of the effect of blood pressure on the development of stroke, acute myocardial infarction, and end-stage renal disease. Hypertens Res 2000; 23: 143–149.

    CAS  Article  Google Scholar 

  8. 8

    Artaud-Wild SM, Connor SL, Sexton G, Connor WE : Differences in coronary mortality can be explained by differences in cholesterol and saturated fat intakes in 40 countries but not in France and Finland. A paradox. Circulation 1993; 88: 2771–2779.

    CAS  Article  Google Scholar 

  9. 9

    Keys A, Menotti A, Aravanis C, et al: The seven countries study: 2,289 deaths in 15 years. Prev Med 1984; 13: 141.

    CAS  Article  Google Scholar 

  10. 10

    Golbitz P : Traditional soy foods: processing and products. J Nutr 1995; 125 ( 3 Suppl): 570S–572S.

    CAS  PubMed  Google Scholar 

  11. 11

    Fujita M, Ito Y, Hong K, Nishimuro S : Characterization of Nattokinase-degraded products from human fibrinogen or cross-linked fibrin. Fibrinolysis 1995; 9: 157.

    CAS  Article  Google Scholar 

  12. 12

    Fujita M, Nomura K, Hong K, Ito Y, Asada A, Nishimuro S : Purification and characterization of a strong fibrinolytic enzyme (nattokinase) in the vegetable cheese natto, a popular soybean fermented food in Japan. Biochem Biophys Res Commun 1993; 197: 1340–1347.

    CAS  Article  Google Scholar 

  13. 13

    Urano T, Ihara H, Umemura K, et al: The profibrinolytic enzyme subtilisin NAT purified from Bacillus subtilis Cleaves and inactivates plasminogen activator inhibitor type 1. J Biol Chem 2001; 276: 24690–24696.

    CAS  Article  Google Scholar 

  14. 14

    Jalil JE, Piddo AM, Cordova S, et al: Prevalence of the angiotensin I converting enzyme insertion/deletion polymorphism, plasma angiotensin converting enzyme activity, and left ventricular mass in a normotensive Chilean population. Am J Hypertens 1999; 12: 697–704.

    CAS  Article  Google Scholar 

  15. 15

    Christian JL, Greger JH : Nutrition for Living. San Francisco, The Benjamin/Cummings Publ Comp Inc, 1991, p 111.

  16. 16

    American Dietetic Association: Handbook of Clinical Dietetics, 2nd ed, New Haven, Yale University Press, 1992, pp 5–39.

  17. 17

    Suzuki Y, Kondo K, Ichise H, Tsukamoto Y, Urano T, Umemura K : Dietary supplementation with fermented soybeans suppresses intimal thickening. Nutrition 2003; 19: 261–264.

    CAS  Article  Google Scholar 

  18. 18

    Suzuki Y, Kondo K, Matsumoto Y, et al: Dietary supplementation of fermented soybean, natto, suppresses intimal thickening and modulates the lysis of mural thrombi after endothelial injury in rat femoral artery. Life Sci 2003; 73: 1289–1298.

    CAS  Article  Google Scholar 

  19. 19

    Fujita M, Hong K, Ito Y, Fujii R, Kariya K, Nishimuro S : Thrombolytic effect of nattokinase on a chemically induced thrombosis model in rat. Biol Pharm Bull 1995; 18: 1387–1391.

    CAS  Article  Google Scholar 

  20. 20

    Pais E, Alexy T, Holsworth RE Jr, Meiselman HJ : Effects of nattokinase, a pro-fibrinolytic enzyme, on red blood cell aggregation and whole blood viscosity. Clin Hemorheol Microcirc 2006; 35: 139–142.

    CAS  PubMed  Google Scholar 

  21. 21

    Maruyama M, Sumi H ( eds): Effect of natto diet on blood pressure, in Basic and Clinical Aspects of Japanese Traditional Food Natto II. Japan Technology Transfer Association (JTTAS), 1998, pp 1–3.

  22. 22

    Kim S, Yamamoto K : The in vivo role of renin-angiotensin system. Cell Science 1992; 8: 146–151.

    CAS  Google Scholar 

  23. 23

    Okamoto A, Hanagata H, Kawamura Y, Yanagida F : Antihypertensive substances in fermented soybean, natto. Plant Foods Hum Nutr 1995; 47: 39–47.

    CAS  Article  Google Scholar 

  24. 24

    Kuba M, Tanaka K, Tawata S, Takeda Y, Yasuda M : Angiotensin I—converting enzyme inhibitory peptides isolated from tofuyo fermented soybean food. Biosci Biotechnol Biochem 2003; 67: 1278–1283.

    CAS  Article  Google Scholar 

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Correspondence to Jong Ho Lee.

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Kim, J., Gum, S., Paik, J. et al. Effects of Nattokinase on Blood Pressure: A Randomized, Controlled Trial. Hypertens Res 31, 1583–1588 (2008). https://doi.org/10.1291/hypres.31.1583

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Keywords

  • nattokinase
  • blood pressure
  • renin activity
  • randomized
  • controlled trial

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