Table 1 Analysis of molecular variance indicated significant population substructure in both B. bicaudatus and B. tricaudatus

From: Genetic diversity and gene flow decline with elevation in montane mayflies

   ct ) Among drainages sc ) Among sites within drainages st ) Among all sites Total
B. bicaudatus df 2 14 585 601
  SumSq 2604.2 9560.7 107740.4 119905.4
  MeanSq 1302.1 682.9 184.2 199.5
  % 1.4% 7.1% 91.4% 100%
  Phi 0.014 0.072 0.086
  P-value 0.5 0.001 0.001
B. tricaudatus df 2 5 221 228
  SumSq 561.6 976.9 23699.2 25137.7
  MeanSq 280.8 175.3 107.2 110.2
  % 1.0% 2.4% 96.6% 100%
  Phi 0.010 0.023 0.034
  P-value 0.057 0.003 0.003
  1. In B. bicaudatus, structure was significant within and among sites, but was not significant at the largest spatial scale (that is, drainages were not a meaningful grouping of genetic variation). In B. tricaudatus, population structure was also significant within and among sites, and was approaching significance even at the largest spatial scale. Only sites with n9 were included in the analysis of molecular variance analysis. P-values were corrected for multiple tests.