Epigenetics investigates heritable changes in gene expression that occur without changes in DNA sequence. Several epigenetic mechanisms, including DNA methylation and histone modifications, can change genome function under exogenous influence. We review current evidence indicating that epigenetic alterations mediate effects caused by exposure to environmental toxicants. Results obtained from animal models indicate that in utero or early-life environmental exposures produce effects that can be inherited transgenerationally and are accompanied by epigenetic alterations. The search for human equivalents of the epigenetic mechanisms identified in animal models is under way. Recent investigations have identified a number of environmental toxicants that cause altered methylation of human repetitive elements or genes. Some exposures can alter epigenetic states and the same and/or similar epigenetic alterations can be found in patients with the disease of concern. On the basis of current evidence, we propose possible models for the interplay between environmental exposures and the human epigenome. Several investigations have examined the relationship between exposure to environmental chemicals and epigenetics, and have identified toxicants that modify epigenetic states. Whether environmental exposures have transgenerational epigenetic effects in humans remains to be elucidated. In spite of the current limitations, available evidence supports the concept that epigenetics holds substantial potential for furthering our understanding of the molecular mechanisms of environmental toxicants, as well as for predicting health-related risks due to conditions of environmental exposure and individual susceptibility.
Epigenetics is the study of heritable changes in gene expression that occur without changes in DNA sequence (Wolffe and Guschin, 2000). Epigenetic mechanisms are flexible genomic parameters that can change genome function under exogenous influence, and also provide a mechanism that allows for the stable propagation of gene activity states from one generation of cells to the next. There are at least two kinds of epigenetic information that can be inherited with chromosomes. The first is DNA methylation, and the second involves changes in chromatin proteins, usually due to modifications in histone tails.
DNA methylation is a covalent modification, heritable by somatic cells after cell division. 5-Methyl-cytosine represents 2–5% of all cytosines in mammalian genomes and is found primarily on CpG dinucleotides (Millar et al., 2003; Lister et al., 2009; Rossella et al., 2009).
Cytosine methylation of CpG dinucleotides is found in close proximity to critically important cis elements within promoters and is often associated with a repressed chromatin state and inhibition of transcription (Orphanides and Reinberg, 2002). DNA methylation also has an important role in the maintenance of genome integrity by transcriptional silencing of repetitive DNA sequences and endogenous transposons (Bestor, 1998; Hedges and Deininger, 2007).
Histones can be modified by acetylation, methylation, phosphorylation, glycosylation, sumoylation and ADP ribosylation (Suganuma and Workman, 2008). The most common modifications are acetylation and methylation of lysine residues in the amino terminal of histone 3 (H3) and histone 4 (H4). Increased acetylation induces transcription activation, whereas decreased acetylation usually induces transcription repression. Methylation of histones is associated with either repression or activation of transcription, depending on the lysine residue position (Yan and Boyd, 2006).
Environmental health and genes: beyond gene–environment interactions
According to the WHO (World Health Organization), more than 13 million deaths annually are due to environmental causes and as much as 24% of disease is caused by exposures that can be averted (Prüss-Üstün and Corvalán, 2006). The list of environmental threats to human health includes a large number of environmental pollutants. For instance, in the third National Report on Human Exposure to Environmental Chemicals by the Center for Disease Control and Prevention, 148 different environmental chemicals that can be detected in the blood and urine were found in a sample of the US population. The list of pollutants evaluated included metals, phytoestrogens, polycyclic aromatic hydrocarbons, dioxin-like chemicals, polychlorinated biphenyls, phthalates and several classes of pesticides (Department of Health and Human Services Centers for Disease Control and Prevention, 2005).
In Figure 1, we propose a categorization of how environmental exposures, with particular interest in environmental toxic chemicals, may interact with genetic and epigenetic mechanisms. In particular, we are interested in contrasting genetic vs epigenetic mechanisms in their possible interplays with environmental exposures.
The interplay between the environment and human genome has been traditionally presented under the framework of gene–environment interactions (Figure 1, path A; also indicated as genotype–environment or G × E interaction) (Ishibe and Kelsey, 1997; Kraft and Hunter, 2005; Dempfle et al., 2008; Baccarelli, 2009; London and Romieu, 2009). Under this model, diseases result from interactions between the individual genetic make-up and environmental factors. Geneticists have always held true that the expression of a genetic trait in the phenotype is highly variable, largely depending on the environment to which the individual carrying the trait of concern is subjected. For instance, in patients with phenylketonuria, which is caused by mutations to a gene coding the liver enzyme phenylalanine hydroxylase, the amino acid phenylalanine does not get converted into tyrosine and reaches high levels in the blood and other tissues (Scriver, 2007). The elevated phenylalanine levels affect brain development leading to mental retardation. However, a low-phenylalanine diet can keep the blood phenylalanine levels low and avoid the severe effects of phenylketonuria.
The same concept can be approached from the realm of environmental health: some individuals have low risk of developing a disease as a result of an environmental exposure, whereas others are much more susceptible. For example, individuals who carry genetic polymorphisms that make their cells less capable of responding to oxidative stress have been found in several investigations to be more susceptible to the cardiovascular and respiratory effects of air pollution, which produces health effects in humans, at least in part, through oxidative stress generation (Park et al., 2006; Chahine et al., 2007; Baccarelli et al., 2008a).
A purely DNA sequence-based approach (naked DNA snapshot) is not sufficient to fully explain the risks of common diseases, which are modulated by other nongenetic or extragenetic mechanisms. In fact, growing evidence shows that the molecular influences of the environment extend well beyond the interaction with the DNA sequence. Several investigations, as we will discuss in the following sections, have shown that environmental toxicants modify epigenetic states.
Gene–environment vs epigene–environment
In gene–environment interactions (Figure 1, path A), the genetic polymorphisms that modify the effects of environmental exposures are transmitted transgenerationally according to Mendelian genetics, and the trait determining effect modifications is generally assumed to follow the same genetic model (dominant, codominant, recessive) as that of the levels of expression or function of the protein coded by the locus of concern. A second well-established area of interplay (Figure 1, path B) includes the direct effects of environmental exposures on the genome, for example, DNA damage and/or mutations induced by environmental exposures. In environmental health, the recognition that exposures could produce DNA mutations represented a major landmark for risk assessment and prevention. Consequently, genotoxic agents have been categorized according to their capability to alter DNA sequence and thus increase disease risk (Siemiatycki et al., 2004). Such information has been fundamental to determine environmental risks and shape current regulatory efforts for exposure reduction. In particular, potential carcinogenic agents have been carefully tested in in vitro and in vivo models of mutagenicity. In human subjects, some of these molecular events may represent early events along the pathways linking carcinogen exposure to cancer. For example, in our own study on the population exposed after the Seveso, Italy accident to high doses of dioxin (Pesatori et al., 2008; Baccarelli et al., 2008b), a powerful promoting carcinogen in animals, we showed an increased number of t(14;18) translocations detectable in phenotypically normal blood lymphocytes collected from healthy subjects (Baccarelli et al., 2006). This effect may represent an early expansion of lymphocyte clones potentially related to the increased risk of non-Hodgkin's lymphoma among subjects exposed to high doses of dioxin (Steenland et al., 2004). Environmentally induced DNA mutations can have a transgenerational effect only if occurring in the germ line. For instance, parental exposure to ionizing radiation has been shown to increase the frequency of germline mutations detectable in the next generation (Charles, 2001), and confer a predisposition to cancer (Dubrova et al., 2000).
In principle, the effect-modification model should apply to epigene–environment interactions and to gene–environment interactions. Similar to the effect modifications shown or postulated for genetic polymorphisms (Figure 1, path A), epigenetic differences determining disease risk could make individuals less or more vulnerable to environmental insults (Figure 1, path C). However, to the best of our knowledge, a formal concept of epigene–environment interaction has not yet been developed and we are not aware of examples of epigene–environment interactions in environmental health or toxicology studies. In environmental studies, the flexibility of epigenetic states has generated a growing interest in evaluating the direct alterations that environmental exposures may produce on epigenetic states (Figure 1, path D), including changes in DNA methylation and histone modifications. Investigations that evaluated alterations in DNA methylation and histone modifications in response to environmental chemical exposures were reviewed by us in a recent article (Baccarelli and Bollati, 2009). In this review, we will discuss the biological basis for potential interplays with epigenetic states that might be activated in the presence of environmental exposures and determine health-related effects. We will also discuss whether available evidence suggests that epigenetics provides biological mechanisms for transgenerational environmental effects.
Epigenetic reprogramming in mammalian development
In mammals, DNA methylation is essential for embryogenesis, during which methylation patterns change dynamically to adapt embryos to be fit for further differentiation (Reik et al., 2001). Two main waves of genome-wide epigenetic reprogramming characterize mammalian development that occurs at the zygote stage and during primordial germ-cell formation (Shi and Wu, 2009).
The genome becomes demethylated during preimplantation to give rise to a totipotent zygote able to generate any cell type. Active DNA demethylation occurring in the paternal genome shortly after fertilization is independent of DNA replication. In contrast, the maternal genome remains highly methylated and undergoes passive DNA demethylation after embryo development (Mayer et al., 2000; Santos et al., 2002). After the first cell cycle, the maternal allele passively loses methylation through cell divisions up to the blastocyst stage (Shi and Wu, 2009). When implantation occurs, DNA methylation levels are then restored by de novo methylation that triggers cell lineage differentiation (Sassone-Corsi, 2002).
The second reprogramming event also occurs during embryogenesis, but only in the primordial germ cells in which DNA methylation patterns are erased at all single copy genes and some repetitive elements (Lees-Murdock and Walsh, 2008).
Similar to the pattern of asymmetric DNA methylation in parental genomes, histone H3K9 trimethylation and dimethylation exhibit asymmetric modifications in the parental pronuclei (Kurdistani et al., 2004; Santos et al., 2005; Valls et al., 2005; Wang et al., 2007; Yoshida et al., 2007).
The dilemma of epigenetic inheritance: can epigenetic marks survive reprogramming?
The cycles of erasure/reprogramming that occur during embryogenesis raise the question about how and how frequently epigenetic marks are inherited transgenerationally, and whether epigenetic inheritance occurs in humans and in animal models (Whitelaw and Whitelaw, 2008). Animal experiments provide us with a few examples suggesting that epigenetic marks that are established during the life of an organism can be passed on to the following generations (Probst et al., 2009). These include epigenetic states of murine genes associated with distinctive phenotypes, such as the agouti locus in the viable yellow (Avy/a) mice (determining fur color variation between yellow and dark brown), the Axin-fused (Axinfu) allele (associated with a kinked tail) (Morgan et al., 1999; Rakyan and Whitelaw, 2003) and the CabpIAP gene, which was identified using a bioinformatic approach and shows sequence homology to the rat CDK5 activator-binding protein (Druker et al., 2004).
All these models derive their properties from metastable epialleles, which are alleles that are variably expressed because of epigenetic modifications that are established very early during development (Rakyan et al., 2002). In metastable epialleles, the epigenetic state can be altered and the alteration shows transgenerational inheritance. Metastable alleles are most often determined by the presence of a retrotransposable element (or retrotransposon). In particular, the murine metastable epialleles Avy, AxinFu and CabpIAP are all associated with contraoriented insertions of a retrotransposable intracisternal A particle (IAP) sequence, a family of retrovirus-like genetic elements coding for virus-like particles (Duhl et al., 1994; Vasicek et al., 1997; Druker et al., 2004).
Epigenetic inheritance and metastable alleles in animal experiments: the viable yellow agouti Avy mouse
The viable yellow agouti Avy allele is the most extensively studied murine metastable epiallele. In viable yellow (Avy/a) mice, transcription originating in an IAP retrotransposon inserted upstream of the agouti gene (A) causes ectopic expression of agouti protein, resulting in yellow fur, obesity, diabetes and increased susceptibility to tumors (Morgan et al., 1999). Avy mice show variable expressivity because they are epigenetic mosaics for activity of the retrotransposon: isogenic Avy mice have coats that vary in a continuous spectrum from full yellow, through variegated yellow/agouti, to full agouti (pseudoagouti). The distribution of phenotypes among offspring is related to the phenotype of the dam; when an Avy dam has the agouti phenotype, her offspring is more likely to be agouti. It has been shown that the offspring color is not the result of a maternally contributed environment, but rather genuinely inherited, and is associated with transmission of a silenced Avy allele through the female germ line (Morgan et al., 1999).
Is there enough evidence for epigenetic inheritance in humans?
Determining whether equivalents of metastable epialleles exist and are frequent in humans poses substantial challenges. There are just a few reports that have been used to suggest inheritance of epigenetic states in humans. For example, in several cases of familial colorectal cancer, the mismatch repair genes MLH1 and MSH2, which usually exhibit low or no methylation, have been found to be silenced because of promoter methylation, and this has been occasionally detected in successive generations (Suter et al., 2004; Chan et al., 2006; Hitchins et al., 2007). However, these were reports of single families and it is has been argued that the promoter methylation identified, even if detected in multiple family members, could be explained by somatic events that occurred after fertilization (Horsthemke, 2007). Whitelaw and Whitelaw (2008) have recently remarked that, in the light of current evidence, the notion that epigenetic marks can be directly inherited across generations in humans remains contentious.
Environmental influences on epigenetic states during early development in animal models
Modifications to the environment during early development can lead to permanent changes in the pattern of epigenetic modifications (Fauque et al., 2007). Pregnant female rats exposed during time of sex determination to the endocrine disruptor vinclozolin have been shown to exhibit in their male offspring transgenerational disease state leading to spermatogenic defects, prostate disease, kidney disease, immune system abnormalities, hypercholesterolemia and an increased rate of tumor development in the F1–F4 generation offspring (Anway et al., 2005, 2006a, 2006b; Anway and Skinner, 2006; Chang et al., 2006). Both the F1 generation embryo and F2 generation germ line are directly exposed when an F0 generation pregnant mother is exposed. Therefore, only the F3 generation can provide the first unequivocal signs of transgenerational inheritance. The transgenerational disease states in the vinclozolin F1–F4 generation animals were found to be associated with a transgenerational alteration in the epigenetic programming of the male germ line (Anway et al., 2005).
A second example of an epigenetic toxicant is represented by bisphenol A (BPA), a high-production-volume chemical used in the manufacture of polycarbonate plastic. In utero or neonatal exposure to BPA is associated with higher body weight, increased breast and prostate cancer, as well as altered reproductive function. Dolinoy et al. (2007a) showed that maternal BPA exposure shifted the coat color distribution of viable yellow agouti (Avy) mouse offspring toward yellow by decreasing CpG (cytosine-guanine dinucleotide) methylation in the IAP sequence upstream of the Agouti gene (Waterland, 2009). In addition, CpG methylation was also decreased at the CabpIAP metastable locus. DNA methylation at the Avy locus was similar in tissues from the three germ layers, suggesting that BPA affected epigenetic patterning during early stem-cell development. Moreover, maternal dietary supplementation with either methyl donors folic acid or the phytoestrogen genistein blunted the DNA-hypomethylating effect of BPA (Dolinoy et al., 2007a). However, as this investigation was conducted only up to the F2 generation, it did not directly show inheritance of the epigenetic modifications induced by BPA exposure.
Translating environmental epigenetic effects from animal models to humans
Translating results obtained in mouse models to humans is not straightforward. The agouti model cannot be directly applied to humans, and neither is there a human equivalent of kinked tails. One common characteristic of the mouse models described above is that their metastable alleles all include an IAP retrotransposon. IAP sequences are endogenous retrovirus-like mobile elements, present at 1000 copies in the mouse genome. These elements transpose in a replicative manner through an RNA intermediate and its reverse transcription, and their transposition should therefore be tightly controlled by their transcription level (Dupressoir and Heidmann, 1997). In humans, more than one-third of DNA methylation occurs in retrotransposons (Kochanek et al., 1993; Schmid, 1998), which represent a large portion of the human genome (Bernstein et al., 2006). Among these sequences, Alu and LINE-1 retrotransposons are the most plentiful families representing ∼30% of the human genome (Kazazian and Goodier, 2002; Grover et al., 2004; Babushok and Kazazian, 2007) and are heavily methylated (Yang et al., 2004). Owing to their high representation throughout the genome, Alu and LINE-1 have been proposed as surrogate markers for estimating the global DNA methylation level (Yang et al., 2004; Weisenberger et al., 2005), but growing evidence indicates that they could have specific and distinct cellular roles (Wallace et al., 2008). Hypomethylation of repetitive elements favors their activity as retrotransposable sequences and has been suggested to have deleterious effects on cells, initially through insertional mutations (Kazazian, 2004), and later by introducing genome instability through deletions and genomic rearrangements (Ostertag and Kazazian, 2001; Gilbert et al., 2002; Wallace et al., 2008).
In healthy populations, inter-individual methylation variations at Alu or LINE-1 elements from blood DNA have also been associated with risk factors for cancer, neurological and cardiovascular diseases (Bollati et al., 2007; Rusiecki et al., 2008; Baccarelli and Bollati, 2009; Baccarelli et al., 2009). In addition, the finding of an age-associated decline in repetitive-element methylation in normal tissues of aging individuals suggests a possible role in various common human age-related diseases (Bollati et al., 2009).
Epigenetics and environmental toxicants in humans
Exposure to air pollution, particularly to particulate matter (PM), has been associated with increased morbidity and mortality from cardiorespiratory disease, as well as with lung cancer risk (Samet et al., 2000; Brook et al., 2004; Peters, 2005; Vineis and Husgafvel-Pursiainen, 2005; Baccarelli et al., 2008c). In a human study, we recently showed that promoter methylation of the iNOS (inducible Nitric Oxide Synthase) gene was lower in blood samples obtained from foundry workers with well-characterized exposure to PM with aerodynamic diameter <10 μm (PM10) (Tarantini et al., 2008). iNOS demethylation is expected to increase the expression and activity of the iNOS protein, which could in turn contribute to inflammation and oxidative stress generation, which are primary mechanisms linking inhalation of air pollutants to their acute health effects (Baccarelli et al., 2007; Chahine et al., 2007; Alexeeff et al., 2008). In the same study, long-term exposure to PM10 was negatively associated with methylation in both Alu and LINE-1 (Tarantini et al., 2008). In a recent investigation, we showed that exposure to black carbon, a marker of particles from vehicular traffic, was also associated with decreased DNA methylation in LINE-1, measured in 1097 blood DNA samples obtained from the NAS (Normative Aging Study), an investigation of elderly men in the Boston area. As repetitive-element hypomethylation is believed to occur in patients with cancer (Ehrlich, 2002) and cardiovascular disease (Castro et al., 2003); such changes may reproduce epigenetic processes related to disease development and represent mechanisms by which particulate air pollution affects human health (Baccarelli et al., 2008c). Several in vitro studies have established an association between DNA methylation and environmental metals, which are components of PM, including nickel, cadmium, lead and particularly arsenic (McVeigh et al., 2001; Bleich et al., 2006; Wright and Baccarelli, 2007; Dolinoy et al., 2007b).
In addition, in an animal study, Yauk et al. (2008) showed that sperm DNA of mice exposed to steel plant air was hypermethylated compared with control animals and this change persisted even after removal from environmental exposure. This finding calls for further research to determine whether air pollutants produce DNA methylation changes that are transmitted transgenerationally.
In our laboratory, we also investigated whether DNA methylation changes are induced by low-benzene exposure in peripheral blood DNA of gasoline station attendants and traffic police officers. High-level exposure to benzene has been associated with increased risk of acute myelogenous leukemia (Snyder, 2002). In our study, airborne benzene exposure was associated with a significant reduction in LINE-1 and Alu methylation. Airborne benzene was also associated with hypermethylation in p15 and hypomethylation of the MAGE-1 cancer-antigen gene (Bollati et al., 2007). These findings show that low-level benzene exposure may induce altered DNA methylation reproducing the aberrant epigenetic patterns found in malignant cells, which in most reports have been found to exhibit repetitive-element hypomethylation as well as either hypermethylation or hypomethylation of specific genes, depending on the gene function. In addition, benzene-associated demethylation of repetitive elements may help explain the epidemiological data linking benzene exposure to increased risk of multiple myeloma (Costantini et al., 2008; Kirkeleit et al., 2008), which also exhibits reduced methylation in Alu and LINE-1 repetitive elements (Bollati et al., 2007).
Other exposures that are associated with increased risk of hematopoietic malignancies, such as persistent organic pollutants, have been associated with changes in repetitive-element DNA methylation. Rusiecki et al. (2008) evaluated the relationship between plasma persistent organic pollutant concentrations and blood global DNA methylation, estimated in Alu repeated elements, and in 70 Greenlandic Inuit, a population presenting some of the highest reported levels of persistent organic pollutants worldwide. In this study, a significant inverse linear relationship was found for DDT, DDE, β-BHC, oxychlordane, α-chlordane, mirex, several PCBs and the sum of all persistent organic pollutants (Rusiecki et al., 2008). As most of the exposures investigated increase oxidative stress in human tissues, it is possible that the production of reactive oxygen species represents a unifying process to account for most of these findings across different chemicals (Wright and Baccarelli, 2007; Baccarelli and Bollati, 2009). Oxidative DNA damage can interfere with the ability of methyltransferases to interact with DNA (Valinluck et al., 2004), thus resulting in a generalized altered methylation of cytosine residues at CpG sites (Turker and Bestor, 1997).
In the last few years, several investigations have examined the relationship between exposure to environmental chemicals and epigenetics, and identified several toxicants that modify epigenetic marks. Most of the studies conducted so far have been focused on DNA methylation, whereas only a few recent investigations have studied the effects of environmental chemicals on histone modifications (Baccarelli and Bollati, 2009). In animal models, environmental effects that might involve epigenetic mechanisms have been shown to be transmitted transgenerationally. Whether the transgenerational epigenetic effects of environmental exposures are present in humans, or whether epigenetic inheritance exists at all in humans, remains to be elucidated.
Nonetheless, growing evidence indicates that epigenetics holds substantial potential for developing biological markers to predict which exposures would put exposed subjects at risk and which individuals will be more susceptible to develop disease. In human studies, this will require the use of laboratory methods with enhanced precision, sensitivity and coverage, so that epigenetic changes can be detected as early as possible and well ahead of disease diagnosis. For several exposures, it has been proven that chemicals can alter epigenetic marks and that the same or similar epigenetic alterations can be found in patients with the disease of concern and/or in diseased tissues. Future prospective investigations are required to determine whether exposed subjects develop epigenetic alterations over time and, in turn, whether such alterations increase the risk of disease.
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The authors declare no conflict of interest.
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Bollati, V., Baccarelli, A. Environmental epigenetics. Heredity 105, 105–112 (2010). https://doi.org/10.1038/hdy.2010.2
- DNA methylation
- histone modifications
- environmental exposures
- transgenerational effects
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