Genetic variation in the clonal bryophyte Hylocomium splendens at hierarchical geographical scales in Scandinavia

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Abstract

The widespread bryophyte Hylocomium splendens was sampled in a hierarchical fashion from populations representing four Scandinavian vegetation zones. Allozyme electrophoresis revealed variation at 11 out of 13 screened loci, allowing accurate identification of genotypes. From a total sample of 298 shoots 79 genotypes could be detected, giving the proportion of distinguishable genotypes (PD) of 0.265. The total allelic diversity (GST) based on polymorphic loci was 0.274. The relative differentiation among populations was low (GST = 0.073), indicating a high level of gene flow. Differences in population structuring occurred between a subarctic-alpine site vs. three lowland sites. The subarctic-alpine population had one widespread clone, which appeared to be propagated by dispersal of vegetative fragments. That population also comprised many rare genotypes, often occurring together within 10 × 10 cm patches. The lowland populations had genotypes that were locally common and often dominant within the patches. When identical genotypes were observed in multiple patches within these populations, it was usually statistically highly probable that they had arisen by independent sexual recombinations.

References

  1. Callaghan, T V, Collins, N J, and Callaghan, C H. 1978. Photosynthesis, growth and reproduction of Hylocomium splendens and Polytrichum commune in Swedish Lapland. Oikos, 31, 73–88.

  2. Derda, G S, and Wyatt, R. 1990. Genetic variation in the common hair-cap moss, Polytrichum commune. Syst Bot, 15, 592–605.

  3. During, H J. 1992. Ecological classification of bryophytes and lichens. In: Bates, J. W. and Farmer, A. M. (eds) Bryophytes and Lichens in a Changing Environment, pp. 1–31. Clarendon Press, Oxford.

  4. Ellstrand, N C, and Roose, M L. 1987. Patterns of genotypic diversity in clonal plant species. Am J Bot, 74, 121–131.

  5. Eriksson, O. 1993. Dynamics of genets in clonal plants. Trends Ecol Evol, 8, 313–316.

  6. Fager, E W. 1972. Diversity: a sampling study. Am Nat, 106, 293–310.

  7. Frego, K A, and Carleton, T J. 1995a. Microsite conditions and spatial pattern in a bryophyte community under mature black spruce. Can J Bot, 73, 544–551.

  8. Frego, K A, and Carleton, T J. 1995b. Microsite tolerance of four bryophytes in a mature black spruce stand: reciprocal transplants. Bryologist, 98, 425–458.

  9. Hamrick, J L, and Godt, M J. 1990. Allozyme diversity in plant species. In: Brown, A. H. D., Clegg, M. T., Kahler, A. L. and Weir, B. S. (eds) Plant Population Genetics, Breeding and Genetic Resources, pp. 3–63. Sinauer Associates, Sunderland, MA.

  10. Hermanutz, L A, Innes, D J, and Weis, I M. 1989. Clonal structure of arctic dwarf birch (Betula glandulosa) at its northern limit. Am J Bot, 76, 755–761.

  11. Jonsson, B G. 1993a. The bryophyte diaspore bank and its role after small-scale disturbance in a boreal forest. J Veg Sci, 4, 819–826.

  12. Jonsson, B G. 1993b. Treefall Disturbance, Succession, and Diversity in Boreal Forest Floor Vegetation. Ph.D. Thesis, University of Umeå.

  13. Kallio, P, and Saarnio, E. 1986. The effect on mosses of transplantation to different latitudes. J Bryol, 14, 159–178.

  14. Jonsson, B O, Jónsdóttir, I S, and Cronberg, N. 1996. Clonal diversity and allozyme variation in populations of the arctic sedge Carex bigelowii (Cyperaceae). J Ecol, 84, 449–459.

  15. Lagercrantz, U, and Ryman, N. 1990. Genetic structure of Norway spruce (Piecea abies): concordance of morphological and allozymic variation. Evolution, 44, 38–53.

  16. Longton, R E, and Schuster, R M. 1983. Reproductive ecology. In: Schuster, R. M. (ed.) New Manual of Bryology, pp. 386–462. Hattori Bot. Lab, Nichinan.

  17. Mårtensson, O. 1956. Bryophytes of the Torneträsk area, Northern Swedish Lapland. II Musci. K Sv Vetenskapsakad Avh naturskydd, 14, 1–321.

  18. Meagher, T R, and Shaw, J. 1989. Clonal structure in the moss, Climacium americanum Brid. Heredity, 64, 233–238.

  19. Mishler, B D. 1988. Reproductive ecology of bryophytes. In: Lovett Doust, J. and Lovett Doust, L. (eds) Plant Reproductive Biology Patterns and Strategies, pp. 285–306. Oxford University Press, Oxford.

  20. Molau, U. 1993. Relationships between flowering phenology and life history strategies in tundra plants. Arctic Alpine Res, 25, 391–402.

  21. Montagnes, R J S, Bayer, R J, and Vitt, D H. 1993. Isozyme variation in the moss Meesia triquetra (Meesiaceae). J Hattori Bot Lab, 74, 155–170.

  22. Myrmael, A. 1993. Regeneration studies of five spruce, Picea abies, forest bryophytes. Lindbergia, 18, 7–18.

  23. Nei, M. 1972. Genetic distance between populations. Am Nat, 106, 238–292.

  24. Nei, M. 1973. Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci, USA, 70, 3321–3323.

  25. Nei, M. 1975. Molecular Population Genetics and Evolution. North Holland, Amsterdam and New York.

  26. Økland, R H. 1994. Bryophyte and lichen persistence patterns in a Norwegian boreal coniferous forest. Lindbergia, 19, 50–62.

  27. Økland, R H. 1995. Population biology of the clonal moss Hylocomium splendens in Norwegian boreal spruce forests. I. Demography. J Ecol, 83, 697–712.

  28. Parks, J C, and Werth, C R. 1993. A study of spatial features of clones in a population of bracken fern, Pteridium aquilinum (Dennstaedtiaceae). Am J Bot, 80, 537–544.

  29. Persson, H, and Viereck, L A. 1983. Collections and discussions of some bryophytes from Alaska. Lindbergia, 9, 5–20.

  30. Pielou, E C. 1969. An Introduction to Mathematical Ecology. Wiley-Interscience, New York.

  31. Schofied, W B. 1985. Introduction to Bryology. Macmillan, New York.

  32. Shaw, A J, and Rooks, P E. 1994. Systematics of Mielichhoferia (Bryaceae: Musci) I. Morphological and genetic analyses of M elongata and M. mielichhoferiana. Bryologist, 97, 1–12.

  33. Soane, I D, and Watkinson, A R. 1979. Clonal variation in populations of Ranunculus repens. New Phytol, 82, 557–573.

  34. Soltis, D E, Haufler, C H, Darrow, D C, and Gastony, G J. 1983. Starch gel electrophoresis of ferns: A compilation of grinding buffers, gel and electrode buffers and staining schedules. Am Fern J, 73, 9–27.

  35. Sonesson, M, Gehrke, C, and Tjus, M. 1992. C02 environment, microclimate and photosynthetic characteristics of the moss Hylocomium splendens in a subarctic habitat. Oecologia, 92, 23–28.

  36. Stoneburner, A, Wyatt, R, and Odrzykoski, I J. 1991. Applications of enzyme electrophoresis to bryophyte systematics and population biology. Adv Bryol, 4, 1–27.

  37. Swofford, D L, and Selander, R B. 1981. BIOSYS-1. A computer program for the analysis of allelic variation in population genetics and biochemical systematics. Release 1.7. University of Illinois, Urbana, IL.

  38. Tamm, C O. 1953. Growth, yield and nutrition in carpets of a forest moss (Hylocomium splendens). Meddn St Skogforsklnstit, 43, 1–140.

  39. Wendel, J F, and Weeden, N F. 1989. Visualization and interpretation of plant isozymes. In: Soltis, D. E. and Soltis, P. S. (eds) Isozymes in Plant Biology, pp. 5–45. Dioscorides Press, Portland, OR.

  40. Widén, B, Cronberg, N, and Widén, M. 1994. Genotypic diversity, molecular markers and spatial distribution in clonal plants, a literature survey. Folia Geobot Phytotax, Praha, 29, 245–263.

  41. Wyatt, R. 1982. Population ecology of bryphytes. J Hattori Bot Lab, 52, 170–198.

  42. Wyatt, R, Odrzykoski, I J, and Stoneburner, A. 1989. High levels of genetic variability in the haploid moss Plagiomnium ciliare. Evolution, 43, 1085–1096.

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Correspondence to Nils Cronberg.

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Keywords

  • allozyme
  • Bryophyta
  • clonal plant
  • genetic diversity
  • hierarchical variation
  • Hylocomium splendens

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