Abstract
The present paper reports the results of cytogenetic analysis carried out on several scattered populations of the striped mullet, Mugil cephalus, the most widespead among mugilid species. The karyotype was investigated through Ag-staining, C-banding, fluorochrome-staining (chromomycin A3/DAPI) and fluorescent in situ hybridization with rDNA genes. All populations showed the same chromosome number and morphology and no changes were detected in heterochromatin and NORs. Therefore, neither population- nor sex-specific marker chromosomes were identified. In some of the specimens, NOR size heteromorphism was detected. Results are discussed with respect to karyotype and ribosomal cistrons organization and to cytotaxonomic implications.
Similar content being viewed by others
Article PDF
References
Amemiya, C T, and Gold, J R. 1986. Chromomycin A3 stains nucleolus organizer regions of fish chromosomes. Copeia, 1986, 226–231.
Arefyev, V A. 1989. The application of the method of colchicine baths to studies of karyotypes of the young of two mullet species (Mugilidae) from the Black Sea. In: Dushkina, L. A. (ed.) Early Life History of Marine Species, pp. 139–149. Sb. Nauchn. Tr. VNIRO Moscow.
Bernardi, G. 1993. Genome organization and species formation in Vertebrates. J Mol Evol, 37, 331–337.
Cano, J, Thode, G, and Alvarez, M C. 1982. Karyoevolutive considerations in 29 Mediterranean teleost fishes. Vie Milieu, 32, 21–24.
Cataudella, S, Civitelli, M V, and Capanna, E. 1974. Chromosome complements of the Mediterranean mullets (Pisces, Perciformes). Caryologia, 27, 93–105.
Chatterjee, K, and Majhi, A. 1973. Chromosomes of Mugil parsia Hamilton (Teleostei, Mugiliformes: Mugilidae). Genen Phaenen, 16, 51–54.
Corti, M, and Crosetti, D. 1995. Geographic variation in the grey mullet Mugil cephalus (Pisces, Mugilidae): a geometric morphometric analysis using partial warp scores. J Fish Biol, (in press).
Crosetti, D, Avise, J C, Placidi, F, Rossi, A R, and Sola, L. 1993. Geographic variability in the grey mullet Mugil cephalus: preliminary results of mtDNA and chromosome analyses. Aquaculture, 111, 95–101.
Crosetti, D, Nelson, W S, and Avise, J C. 1994. Pronounced genetic structure of mitochondrial DNA among populations of the circumglobally distributed grey mullet (Mugil cephalus). J Fish Biol, 44, 47–58.
Delgado, J V, Thode, G, Lobillo, J, Camacho, M E, Alonso, A, and Rodero, A. 1991. Detección de la región del organizador nucleolar en cromosomas de la familia Mugilidae (Perciformes): precisiones técnicas. Arch Zootec, 40, 301–305.
Howell, W M, and Black, D A. 1980. Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia, 36, 1014–1015.
Jordão, L C, Oliveira, C, Foresti, F, and Godinho, H M. 1992. Caracterização citogenética da tainha, Mugil platanus (Pisces, Mugilidae). B Inst Pesca, 19, 63–66.
Khuda-Bukhsh, A R, and Manna, G K. 1974. Somatic chromosomes in seven species of teleostean fishes. Chrom Inf Serv, 17, 5–6.
Lawrence, J B, Villnave, C A, and Singer, R H. 1988. Sensitive, high-resolution chromatin and chromosome mapping in situ: presence and orientation of two closely integrated copies of EBV in a lymphoma line. Cell, 52, 51–61.
Legrande, W H, and Fitzsimons, J M. 1976. Karyology of the mullets Mugil curema and M. cephalus (Perciformes: Mugilidae) from Louisiana. Copeia, 1976, 388–391.
Natarajan, R, and Subrahmanyam, K. 1974. A karyotype study of some teleosts from Portonovo waters. Proc Indian Acad Sci, 79B, 173–196.
Nayyar, R P. 1966. Karyotype studies in thirteen species of fishes. Genetica, 37, 78–92.
Ohno, S. 1974. Protochordata, Cyclostomata and Pisces. In: John, B. (ed.) Animal Cytogenetics, vol. 4. Gebrüder Borntraeger, Berlin.
Pauls, E, and Coutinho, I A. 1990. Levantamento citogenetico em peixes de maior valor econömico do litoral Fluminense, RJ (23° Lat/S). Congresso Brasileiro de Zoologia, 17, P. 325. Universidade Estadual de Londrina, 1990.
Pendás, A M, Morán, P, and GarcÃa-Vázquez, E. 1993a. Multi-chromosomal location of ribosomal RNA genes and heterochromatin association in brown trout. Chromosome Res, 1, 63–67.
Pendás, A M, Moran, P, and GarcÃa-Vázquez, E. 1993b. Ribosomal RNA genes are interspersed throughout a heterochromatic chromosome arm in Atlantic salmon. Cytogenet Cell Genet, 63, 128–130.
Phillips, R B, and Hartley, S E. 1988. Fluorescent banding patterns of the chromosomes of the genus Salmo. Genome, 30, 193–197.
Phillips, R B, Pleyte, K A, and Ihssen, P E. 1989. Patterns of chromosomal nucleolar organizer region (NOR) variation in fishes of the genus Salvelinus. Copeia, 1989, 47–53.
Rishi, K K, and Singh, J. 1982. Karyological studies on five estuarine fishes. Nucleus, 25, 178–180.
Rossi, A R, Capula, M, Crosetti, D, Sola, L, and Campton, D E. 1995. Allozyme variation and genetic differentiation among global populations of striped mullet Mugil cephalus (Pisces: Mugilidae). Copeia (in press).
Sola, L, Iaselli, V, Rossi, A R, Rasch, A R, and Monaco, P J. 1992a. Cytogenetics of bisexual/unisexual species of Poecilia. III. The karyotype of Poecilia formosa, a gynogenetic species of hybrid origin. Cytogenet Cell Genet, 60, 236–240.
Sola, L, Rossi, A R, Iaselli, V, Rasch, E M, and Monaco, P J. 1992b. Cytogenetics of bisexual/unisexual species of Poecilia. II. Analysis of heterochromatin and nucleolar organizer regions in Poecilia mexicana mexicana by C-banding and DAPI, quinacrine, chromomycin A3, and silver staining. Cytogenet Cell Genet, 60, 229–234.
Sola, L, Bressanello, S, Rasch, E M, and Monaco, P J. 1993. Cytogenetics of bisexual/unisexual species of Poecilia. IV. Sex chromosomes, sex chromatin composition and Ag-NOR polymorphisms in Poecilia latipinna: a population from Mexico. Heredity, 70, 67–71.
Sumner, A T. 1972. A simple technique for demonstrating centromeric heterochromatin. Exp Cell Res, 75, 304–306.
Thomson, J M. 1963. Synopsis of biological data on the grey mullet (Mugil cephalus Linnaeus, 1758). Fisheries Synopsis 1, Division of Fisheries and Oceanography, CSIRO Australia.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rossi, A., Crosetti, D., Gornung, E. et al. Cytogenetic analysis of global populations of Mugil cephalus (striped mullet) by different staining techniques and fluorescent in situ hybridization. Heredity 76, 77–82 (1996). https://doi.org/10.1038/hdy.1996.10
Received:
Issue Date:
DOI: https://doi.org/10.1038/hdy.1996.10
Keywords
This article is cited by
-
Molecular cytogenetic study of genome ploidy in the German mirror carp Cyprinus carpio
Fisheries Science (2014)
-
A global review of the cosmopolitan flathead mullet Mugil cephalus Linnaeus 1758 (Teleostei: Mugilidae), with emphasis on the biology, genetics, ecology and fisheries aspects of this apparent species complex
Reviews in Fish Biology and Fisheries (2012)
-
Molecular Phylogeny of Grey Mullets (Teleostei: Mugilidae) in Greece: Evidence from Sequence Analysis of mtDNA Segments
Biochemical Genetics (2007)
-
Comparative Molecular Cytogenetic Analysis of Two Congeneric Species, Mugil Curema and M. Liza (Pisces, Mugiliformes), Characterized by Significant Karyotype Diversity
Genetica (2005)
-
Banded karyotype of the Greek endemic cyprinid fish,Pachychilon macedonicum
Ichthyological Research (2000)
