Inheritance of RAPD fragments in haploid and diploid tissues of Pinus sylvestris (L.)


We studied the inheritance of RAPD fragments in haploid and diploid tissues of eight parents and 80 diploid controlled F1 progeny of Pinus sylvestris (L.). Most fragments detected in the diploid tissue of parents were also present in their haploid macrogametophytes. All fragments observed in the parents were found in the progeny. Individual fragments amplified by a single primer were observed in unrelated trees suggesting that primer competition is not likely to cause ‘epistatic’ effects on RAPD polymorphism. Segregation of all variable fragments observed in haploid and diploid materials fitted the proportions expected for a dominant Mendelian trait. The segregating fragments were either present or absent confirming the dominant character of RAPD variation reported in most previous studies. None of the fragments detected in this study appeared to originate from uniparentally inherited cytoplasmic DNA. Our present results provide additional support for the usefulness of RAPD fragments as genetic markers. Nevertheless, the few differences observed between haploid and diploid tissues with respect to some fragments emphasize the need for inheritance studies preceding the use of RAPD fragments as genetic markers.


  1. Bartels, H. 1971. Genetic control of multiple esterases from needles and macro-gametophytes of Picea abies. Planta, 99, 283–289.

  2. Bucci, G, and Menozzi, P. 1993. Segregation analysis of random amplified polymorphic DNA (RAPD) markers in Picea abies Karst. Mol Ecol, 2, 227–232.

  3. Carlson, J E, Tulsieram, L K, Glaubitz, J C, Luk, V W K, Kauffeldt, C, and Rutledge, R. 1991. Segregation of random amplified DNA markers in F1 progeny of conifers. Theor Appl Genet, 83, 194–200.

  4. Chaparro, J X, Werner, D J, O'Malley, D, and Sederoff, R R. 1994. Targeted mapping and linkage analysis of morphological isozyme and RAPD markers in peach. Theor Appl Genet, 87, 805–815.

  5. Dong, J S, and Wagner, D B. 1994. Paternally inherited chloro-plast polymorphism in Pinus: estimation of diversity and population subdivision, and tests of disequilibrium with a maternally inherited mitochondrial polymorphism. Generics, 136, 1187–1194.

  6. Doyle, J J, and Doyle, J L. 1990. Isolation of plant DNA from fresh tissue. Focus, 12, 13–15.

  7. Echt, C S, Erdahl, L A, and McCoy, T J. 1992. Genetic segregation of random amplified polymorphic DNA in diploid cultivated alfalfa. Genome, 35, 84–87.

  8. Ennos, R. 1994. Estimating the relative rates of pollen and seed migration among plant populations. Heredity, 72, 250–259.

  9. Heun, M, and Helentjaris, T. 1993. Inheritance of RAPDS in F1 hybrids of corn. Theor Appl Genet, 85, 961–968.

  10. Hilu, K W. 1994. Evidence from RAPD markers in the evolution of Echinochloa millets (Poaceae). Plant Syst Evol, 189, 247–257.

  11. Huff, D R, Peakall, R, and Smouse, P E. 1993. RAPD variation within and among natural populations of outcrossing buffalograss [Buchloe dactyloides (Nutt) Engelm]. Theor Appl Genet, 86, 927–934.

  12. Hunt, G J, and Page, R E. 1992. Patterns of inheritance with RAPD molecular markers reveal novel types of polymorphism in the honey bee. Theor Appl Genet, 85, 15–20.

  13. Kaya, Z, and Neale, D B. 1993. Random amplified polymorphic DNA (RAPD) polymorphisms in Pinus nigra subsp. pallasiana and Pinus brutia. Turkish J Agric Forestry, 17, 295–306.

  14. Kazan, K, Manners, J M, and Cameron, D F. 1993. Inheritance of random amplified polymorphic DNA markers in an interspecific cross in the genus Stylosanthes. Genome, 36, 50–56.

  15. Lindgren, D, and Wang, Q-Y. 1986. Are genetic results influenced by the environment during seed maturation? In: Lindgren, D. (ed.) Joint Meeting of IUFRO WP on Breeding Theory, Progeny Testing and Seed Orchards, 13–17 October, 1986, Umea, Sweden, pp. 192–199. Swedish University of Agricultural Sciences.

  16. Liu, Z, and Furnier, G R. 1993. Comparison of allozyme, RFLP, and RAPD markers for revealing genetic variation within and between trembling aspen and bigtooth aspen. Theor Appl Genet, 87, 97–105.

  17. Morris, R W, and Spieth, P T. 1978. Sampling strategies for using female gametophytes to estimate heterozygosities in conifers. Theor Appl Genet, 51, 217–222.

  18. Neale, D B, and Sederoff, R R. 1989. Paternal inheritance of chloroplast DNA and maternal inheritance of mitochondrial DNA in loblolly pine. Theor Appl Genet, 77, 212–216.

  19. Nelson, C D, Nance, W L, and Doudrick, R L. 1993. A partial genetic linkage map of slash pine (Pinus elliottii Engelm. var. elliottii) based on random amplified polymorphic DNAS. Theor Appl Genet, 87, 145–151.

  20. Quiros, C F, Ceada, A, Georgescu, A, and Hu, J. 1993. Use of RAPD markers in potato genetics: segregations in diploid and tetraploid families. Am Potato J, 70, 35–42.

  21. Rothuizen, J, and Van Wolferen, M. 1994. Randomly amplified DNA polymorphisms in dogs are reproducible and display Mendelian transmission. Animal Genet, 25, 13–18.

  22. Rowland, L J, and Levi, A. 1994. RAPD-based genetic linkage map of blueberry derived from a cross between diploid species (Vaccinium darrowi and V. elliottii). Theor Appl Genet, 87, 863–868.

  23. Roy, A, Frascaria, N, Mackay, J, and Bousouet, J. 1992. Segregating random amplified polymorphic DNAs (RAPDs) in Betula alleghaniensis. Theor Appl Genet, 85, 173–180.

  24. Schaffer, H E, and Sederoff, R. 1981. Improved estimation of DNA fragment lengths from agarose gels. Analyt Biochem, 115, 113–122.

  25. Suzuki, R, Kemp, S J, and Teale, A J. 1993. Polymerase chain reaction analysis of mitochondrial DNA polymorphism in Ndama and Zebu cattle. Animal Genet, 24, 339–343.

  26. Szmidt, A E, Aldén, T, and Hallgren, J-E. 1987. Paternal inheritance of chloroplast DNA in Larix. Plant Mol Biol, 9, 59–64.

  27. Taberlet, P, Gielly, L, Pautou, G, and Bouvet, J. 1991. Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol Biol, 17, 1105–1109.

  28. Tulsieram, L K, Glaubitz, J C, Kiss, G, and Carlson, J E. 1992. Single tree genetic linkage mapping in conifers using haploid DNA from megagametophytes. Bio/Technology, 10, 686–690.

  29. Wang, X-R, and Szmidt, A E. 1994. Chloroplast DNA variation in a Pinus species complex from Asia. Evolution, 48, 1020–1031.

  30. Williams, J G K, Kubelik, A R, Livak, K J, Rafalski, J A, and Tingey, S V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acids Res, 18, 6531–6535.

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Correspondence to Alfred E Szmidt.

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  • controlled cross
  • diploid bud
  • haploid macrogametophyte
  • inheritance
  • Pinus sylvestris
  • RAPDs

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