Fluorescence in situ hybridization (FISH) was used to localize the 18S-28S ribosomal RNA gene clusters on the chromosomes of 15 mosquito species belonging to the Anophelinae and Culicinae subfamilies. In the genus Anopheles the rRNA genes are localized on the heterochromatic arm of both sex chromosomes. The association between rRNA genes and sex determining chromosomes also applies to the homomorphic karyotype of Culicinae mosquitoes, at least in those cases in which localization of the sex locus/loci has been determined. In these species ribosomal genes are often found within or adjacent to heterochromatic regions (C bands). Differences in the location of rRNA genes among and within genera suggest the occurrence of several chromosomal rearrangements during the evolution of mosquitoes.
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We are grateful to William C. Black for kindly providing the 18S rRNA probe, Frank Speleman and Roberta Vanni for technical advice on the FISH technique, Leonard E. Munstermann for helping in the collection of Orthopodomyia mosquitoes and Louis G. Mukwaya for the colony of Er. quinquevittatus.
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Marchi, A., Pili, E. Ribosomal RNA genes in mosquitoes: localization by fluorescence in situ hybridization (FISH). Heredity 72, 599–605 (1994). https://doi.org/10.1038/hdy.1994.83
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