Fluorescence in situ hybridization (FISH) was used to localize the 18S-28S ribosomal RNA gene clusters on the chromosomes of 15 mosquito species belonging to the Anophelinae and Culicinae subfamilies. In the genus Anopheles the rRNA genes are localized on the heterochromatic arm of both sex chromosomes. The association between rRNA genes and sex determining chromosomes also applies to the homomorphic karyotype of Culicinae mosquitoes, at least in those cases in which localization of the sex locus/loci has been determined. In these species ribosomal genes are often found within or adjacent to heterochromatic regions (C bands). Differences in the location of rRNA genes among and within genera suggest the occurrence of several chromosomal rearrangements during the evolution of mosquitoes.
Bedo, D G, and Webb, G C. 1989. Conservation of nucleolar structure in polytene tissues of Ceratitis capitata (Diptera: Tephritidae). Chromosoma, 98, 443–449.
Beckingham, K, and Rubacha, A. 1984. Different chromatin states of the intron − and type 1 intron+ rRNA genes of Calliphora erythrocephala. Chromosoma, 90, 311–316.
Black, W C. iv. McLain, D K, and Rai, K S. 1989. Patterns of variation in the rRNA cistron within and among world populations of a mosquito Aedes albopictus (Skuse). Genetics, 121, 539–550.
Coluzzi, M, Sabatini, A, Petrarca, V, and Di Deco, M A. 1979. Chromosomal differentiation and adaptation to human environments in the Anopheles gambiae complex. Trans R Soc Trop Med Hyg, 73, 483–497.
Dennhöfer, L. 1972. The correlation of linkage groups with chromosomes in the mosquito, Culex pipiens L. Chromosoma, 37, 43–52.
Endow, S A, and Gall, J G. 1975. Differential replication of satellite DNA in polyploid tissues of Drosophila virilis. Chromosoma, 50, 175–192.
Gale, K R, and Crampton, J M. 1989. The ribosomal genes of the mosquito, Aedes aegypti. Eur J Biochem, 185, 311–317.
Gambarini, A G, and Lara, F J S. 1974. Under-replication of ribosomal cistrons in polytene chromosomes of Rhynchosciara. J Cell Biol, 62, 215–222.
Gillings, M R, Frankham, R, Speirs, J, and Whalley, M. 1987. XY exchange and the coevolution of the X and Y rDNA arrays in Drosophila melanogaster. Genetics, 116, 241–251.
Grimm, C, Kunz, W, and Franz, G. 1984. The organ-specific rRNA gene number in Drosophila hydei is controlled by sex heterochromatin. Chromosoma, 89, 48–54.
Hägele, K, and Ranganath, H A. 1983. The chromosomes of two Drosophila races: Drosophila nasuta nasuta and D. n. albomicans. III. Localization of nucleolar organizer regions. Genetica, 60, 123–128.
James, A A. 1992. Mosquito molecular genetics: the hands that feed bite back. Science, 257, 37–38.
Kitzmiller, J B, Frizzi, G, and Baker, R H. 1967. Evolution and speciation within the Maculipennis complex of the genus Anopheles. In: Wright, J. W. and Pal, R. (eds) Genetics of Insect Vectors of Disease, pp. 151–120. Elsevier, Amsterdam.
Knight, K L, and Stone, A. 1977. A catalog of the mosquitoes of the world (Diptera: Culicidae). The Thomas Say Foundation, VI. Baltimore, MD.
Kumar, A, and Rai, K S. 1990. Chromosomal localization and copy number of 18S + 28S ribosomal RNA genes in evolutionarily diverse mosquitoes (Diptera: Culicidae). Hereditas, 113, 277–289.
Lyckegaard, E M, and Clark, A G. 1989. Ribosomal DNA and stellate gene copy number variation on the Y chromosome of Drosophila melanogaster. Proc Natl Acad Sci USA, 86, 1944–1948.
Marchi, A. 1988. Chromosomal evolution in the genus Orthopodomyia (Diptera: Culicidae). Abstracts XVIII International Congress on Entomology, p. 15. Vancouver, Canada.
Marchi, A, and Mezzanotte, R. 1990. Inter-and intraspecific heterochromatin variation detected by restriction endo-nuclease digestion in two sibling species of the Anopheles maculipennis complex. Heredity, 65, 135–142.
Mezzanotte, R, Ferrucci, L, and Contini, C. 1979. Identification of sex chromosomes and characterization of the heterochromatin in Culiseta longiareolata (Macquart, 1838). Genetica, 50, 135–139.
Miller, O J. 1981. Nucleolar organizers in mammalian cells. In: Bennett, M. D., Bobrow, M. and Hewitt, G. M. (eds), Chromosomes Today, pp. 64–73. Allen&Unwin, Boston.
Motara, M A, Pathak, S, Satya-Prakash, K L, and Hsu, T C. 1985. Argentophilic structures of spermatogenesis in the yellow fever mosquito. J Hered, 76, 295–300.
Munstermann, L E, Marchi, A, Sabatini, A, and Coluzzi, M. 1985. Polytene chromosomes of Orthopodomyia pulchripalpis (Diptera, Culicidae). Parasitologia, 27, 267–277.
Pardue, M L. 1985. In situ hybridization. In: Hanes, B. D. and Higgins, S. J. (eds) Nucleic Acid Hybridization: a Practical Approach, pp. 179–202. IRL Press, Oxford.
Pardue, M L, Gerbi, S A, Eckhardt, R A, and Gall, J G. 1970. Cytological localization of DNA complementary to ribosomal RNA in polytene chromosomes of Diptera. Chromosoma, 29, 268–290.
Park, Y, and Fallon, A M. 1990. Mosquito ribosomal RNA genes: characterization of gene structure and evidence for changes in copy number during development. Insect Biochem, 20, 1–11.
Rao, P N, and Rai, K S. 1987. Comparative karyotypes and chromosomal evolution in some genera of nematocerous (Diptera: Nematocera) families. Ann Entomol Soc Am, 80, 321–332.
Renkawitz, R, and Kunz, W. 1975. Independent replication of the ribosomal RNA genes in the polytrophic meroistic ovaries of Calliphora erythrocephala, Drosophila hydei and Sarcophaga barbata. Chromosoma, 53, 131–140.
Sakai, R K, Baker, R H, Raana, K, and Hassan, M. 1979. Crossing-over in the long arm of the X and Y chromosomes in Anopheles culicifacies. Chromosoma, 74, 209–218.
Spear, B. 1974. The genes for ribosomal RNA in diploid and polytene chromosomes of Drosophila melanogaster. Chromosoma, 48, 159–179.
Speleman, F, Van Der Auwera, B, Mangelschots, K, Vercruyssen, M, Raap, T, Wiegant, J, Craen, M, and Lery, J. 1990. Identification and characterization of normal length nonfluorescent Y chromosomes: cytogenetic analysis, Southern hybridization and non-isotopic in situ hybridization. Hum Genet, 85, 569–575.
Sinibaldi, R M, and Cummings, M R. 1981. Localization and characterization of rDNA in Drosophila tumiditarsus. Chromosoma, 81, 655–671.
Sumner, A T. 1990. Chromosome Banding. Unwin Hyman, London.
Vitelli, L, Batistoni, R, Andronico, F, Nardi, I, and Barsacchi-Pilone, G. 1982. Chromosomal localization of 18S + 28S and 5S ribosomal RNA genes in evolutionarily diverse anuran amphibians. Chromosoma, 84, 475–491.
White, G B. 1980. Academic and applied aspects of mosquito cytogenetics. In: Blackman, R. L., Hewitt, G. M. and Ashburner, M. (eds) Insect Cytogenetics, pp. 245–274. Blackwell Scientific Publications, Oxford.
Zacharias, H. 1984. Allocyclic behaviour and under-replication of the nucleolus chromosome in Pseudodiamesa (Chironomidae). Chromosoma, 89, 263–273.
Zavortink, T J. 1968. Mosquito studies. VIII. A prodrome of the genus Orthopodomyia. Contr Amer Entomol Inst, 3, 1–221.
We are grateful to William C. Black for kindly providing the 18S rRNA probe, Frank Speleman and Roberta Vanni for technical advice on the FISH technique, Leonard E. Munstermann for helping in the collection of Orthopodomyia mosquitoes and Louis G. Mukwaya for the colony of Er. quinquevittatus.
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Marchi, A., Pili, E. Ribosomal RNA genes in mosquitoes: localization by fluorescence in situ hybridization (FISH). Heredity 72, 599–605 (1994). https://doi.org/10.1038/hdy.1994.83
- mosquito chromosomes
- rRNA genes
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