An examination of the meiotic pattern of chromosome 1 isolated from a feral mouse population and containing a double insertion (Is) of homogeneously-staining regions (HSRs) was carried out. In a previous study is was shown that the region delineated by the proximal breakpoint of Is(HSR;1C5) 1Icg and the distal one of Is(HSR;1D)2Icg is unpaired during early pachytene and heterosynapsed at midpachytene. No synaptic disturbances were revealed in homozygotes in this study. Chiasmata number per first bivalent in heterozygous (1.87) and homozygous (1.88) males was shown to be higher than in normal ones (1.61). In normal males a single chiasma is located in the medial part of chromosome 1. In heterozygotes this segment is heterosynapsed and unavailable for recombination. This leads to a significant decrease in the frequency of bivalents bearing a single chiasma and an increase in the frequency of bivalents bearing double chiasmata located mostly at subcentromeric and subtelomeric regions of the chromosome. In homozygous males the frequency of double chiasmata is also increased, and even triple chiasmata become possible because of the increase in the physical length of the bivalents. Thus insertion of heterochromatic regions, which are inert with respect to recombination, leads to an increase in the length of the genetic map of the chromosome because of relaxation of interference restrictions.
Agulnik, S I, Borodin, P M, Gorlov, I P, Yu Ladygina, T, and Pak, S D. 1993. Causes of appearance of a double insertion of homogeneously staining regions in the house mouse (Mus musculus musculus). Heredity, 65, 265–271.
Agulnik, S I, Gorlov, I P, and Agulnik, A I. 1987. Mouse Chr 1 with two insertions: Is(HSR;1C5)lIcg and 1s(HSR; 1E3)21cg. Mouse News Lett, 78, 70.
Agulnik, S I, Gorlov, I P, and Agulnik, A I. 1988. New variant of chromosome 1 of the house mouse. Cytologiya, 30, 773–775. (in Russian).
Arana, P, Santos, J L, and Giraldez, R. 1980. Chiasma interference and centromere co-orientation on spontaneous translocation heterozygote of Eucherthippus pulvinatus gallicus (Acrididae; Orthoptera). Chromosoma, 78, 327–340.
Boldyreff, B, Weith, A, Winking, H, Parmann, L, and Traut, W. 1988. Amplification and rearrangement in a germ line HSR of the mouse. Genet Res, 52, 65–70.
Borodin, P M, Gorlov, I P, and Yu Ladygina, T. 1990. Double insertion of homogeneously staining regions in chromosome 1 of wild Mus musculus musculus: effects on chromosome pairing and recombination. J Hered, 81, 91–95.
Chandley, A C. 1986. A model for effective pairing and recombination at meiosis based on early replicating sites (R-bands) along chromosomes. Hum Genet, 72, 50–57.
Egel, R. 1978. Synaptonemal complex and crossing over: structural support or interference? Heredity, 43, 233–237.
Egel-Mitani, M, Olson, L W, and Egel, R. 1982. Meiosis in Aspergillus nidulans: another example for lacking synaptonemal complexes in the absence of crossover interference. Hereditas, 97, 179–188.
Evans, E P, Breckon, G, and Ford, C D. 1964. An air-drying method for meiotic preparations from mammalian testes. Cytogenetics, 3, 289–299.
Forejt, J. 1973. Centromeric heterochromatin polymorphism in the house mouse. Evidance from inbred strains and natural populations. Chromosoma, 43, 187–201.
Fox, D P. 1973. The control of chiasma distribution in the locust, Schistocerca gregaria (Forskal). Chromosoma, 65, 247–267.
Gorlov, I P, Agulnik, A I, and Agulnik, S I. 1987. Localization of house mouse genes on chromosomes via comparison of the genetic map and the chiasmata distribution. Genetika, 23, 63–70 (in Russian).
Henderson, S A. 1963. Chiasma distribution at diplotene in a locust. Heredity, 18, 173–190.
Holliday, R. 1977. Genetic recombination and meiosis. Philos Trans R Soc London B, 277, 359–370.
Howell, W M, and Black, D A. 1980. Controlled silver-staining of nucleolus organizer-regions with protective colloidal developer: a 1-step method. Experientia, 36, 1014–1015.
John, B. 1976. Myth and mechanisms of meiosis. Chromosoma, 54, 295–325.
John, W, and King, M. 1985. The inter-relationship between heterochromatin distribution and chiasma distribution. Genetica, 66, 183–194.
Kalisch, W E. 1975. Tandem duplication in Drosophila melanogaster.II. Meiotic pairing and exchange in heterozygous tandem duplications. Theoret Appl Genet, 46, 169–180.
King, R C. 1970. The meiotic behaviour of the Drosophila oocyte. Int Rev Cytol, 28, 125–165.
Mather, K. 1937. The determination of position in crossing-over.II. The chromosome length-chiasma frequency relation. Cytologia Fujii Jubilee Vol, 514–526.
Mather, K. 1938. Crossing-over. Biol Rev, 13, 252–292.
Moses, M J. 1980. New cytogenetic studies on mammalian meiosis. In: Serio, M. and Martini, L. (eds) Animal Models in Human Reproduction. Academic Press, London, pp. 169–190.
Novitski, E, and Braver, G. 1954. An analysis of crossing over within a heterozygous inversion in Drosophila melanogaster. Genetics, 39, 197–209.
Olson, L W, Eden, U, Egel-Mitani, M, and Egel, R. 1978. Asynaptic meiosis in fission yeast. Hereditas, 89, 189–199
Parker, J S. 1987. Increased chiasma frequency as a result of chromosome rearrangement. Heredity, 58, 87–94.
Parker, J S, Palmer, R W, Whitehorn, M A F, and Edgar, L A. 1982. Chiasma frequency effects of structural chromosome change. Chromosome, 85, 673–686.
Rhoades, M M. 1968. Studies on the cytological basis of crossing-over. Peacok, W. J. and Brock, R. D. (eds). Replication and Recombination of Genetic Material. Australian Academy of Science, Canberra, pp. 229–241.
Roberts, P A. 1970. Screening for X-ray-induced crossover suppressors in Drosophila melanogaster. prevalence and effectiveness of translocations. Genetics, 65, 429–448.
Stack, S. 1984. Heterochromatin, the synaptonemal complex and crossing-over. J Cell Sci, 11, 159–176.
Sturtevant, A H. 1926. A crossover reducer in Drosophila melanogaster due to an inversion of a section of the third chromosome. Biol Zenir Bl, 46, 697–702.
Sumner, A T. 1972. A simple technique for demonstrating centromeric heterochromatin. Exp Cell Res, 75, 304–306.
Sybenga, J. 1975. Meiotic configurations. In: Frankel, R. (ed.). Monographs on Theoretical and Applied Genetics 1. Springer Verlag, Berlin, pp. 1–251.
Traut, W, Winking, H, and Adolph, S. 1984. An extra segment in chromosome 1 of wild Mus musculus: C-band positive homogeneously staining region. Cytogenet Cell Genet, 38, 290–297.
Wettstein, P, Rasmussen, S W, and Holm, P B. 1984. The synaptonemal complex in genetic segregation. Ann Rev Genet, 18, 331–413.
The research was supported by the Russian State Programme 'Frontiers in Genetics'. We are extremely grateful to an anonymous reviewer for helpful comments on the manuscript and to Mrs Marina I. Rodionova for technical assistance.
Rights and permissions
About this article
Cite this article
Gorlov, I., Ladygina, T. & Borodin, P. Chiasma distribution in the first bivalent of mice carrying a double insertion of homogeneously-staining regions in homo- and heterozygous states. Heredity 70, 642–647 (1993). https://doi.org/10.1038/hdy.1993.92
This article is cited by
On the origin of crossover interference: A chromosome oscillatory movement (COM) model
Molecular Cytogenetics (2011)
Chiasma localization, heterochromatin and synaptonemal complexes in the grasshopperPyrgomorpha conica
Chromosome Research (1996)
Recombination in single and double heterozygotes for two partially overlapping inversions in chromosome 1 of the house mouse