A wild sample of Adalia bipunctata larvae and pupae were collected from Surrey, and F1 virgin adults mated. The sex ratio produced by these pairs was found be be variable, 22 pairs produced a sex ratio consistent with a 1:1 sex ratio, but four produced a strong female bias. Daughters and males from biased broods were mated to adults from non-biased broods of different parentage. Males and females from unrelated families were crossed. Where the female was taken from a biased clutch, the biased sex ratio trait recurred in a high proportion of cases. Where males from such broods were crossed, or females from broods with no history of bias were mated, sex ratios consistent with 1:1 were obtained. Pairs producing a strong female bias showed hatch rates which were approximately half that of crosses producing non-biased sex ratios. Treatment with tetracycline in golden syrup cured females who showed the biased sex ratio trait. Daughters conceived after this treatment produced normal sex ratios. Females fed purely on golden syrup, as a control, continued to produce female biased sex ratios until treated with tetracycline. We therefore suggest that an unknown bacterium, transmitted through egg cytoplasm but not sperm, is responsible for the early mortality of male embryos and thus a bias in the secondary sex ratio. The contribution that the study of the Coccinellidae can make to theories concerning the incidences and evolution of male killing elements is discussed in relation to egg cannibalism.
Andreadis, T G. 1985. Experimental transmission of a microsporidian pathogen from mosquitoes to an alternate copepod host. Proc Natl Acad Sci, USA, 82, 5574–5577.
Andreadis, T G, and Hall, D W. 1979. Significance of trans-ovariol transmission of Amblyospora sp in relation to parasite maintenance in the mosquito Culex salinarius. J Invert Path, 34, 152–157.
Banks, C J. 1956. Observations on the behaviour and early mortality of coccinellid larvae before dispersal from egg shells. Proc Roy Ent Soc Lond A, 31, 56–61.
Birky, C W, Jr 1978. Transmission genetics of mitochondria and chloroplasts. Ann Rev Genet, 12, 472–512.
Charnov, E L. 1982. The Theory of Sex Allocation. Princeton University Press, New Jersey.
Clarke, C, Johnston, G, and Johnston, B. 1983. All female broods in Hypolimnas bolina (L.) A resurvey of West Fiji after 60 years. Biol J Linn Soc, 19, 221–235.
Cosmides, L M, and Tooby, J. 1981. Cytoplasmic inheritance and intragenomic conflict. J Theor Biol, 89, 83–129.
Ebbert, M. 1991. The interaction phenotype in the Drosophila willistoni — spiroplasma symbiosis. Evolution, 45, 971–988.
Frank, S A. 1989. The evolutionary dynamics of cytoplasmic male sterility. Am Nat, 133, 345–376.
Frank, S A. 1991. Divergence of meiotic drive suppression systems as an explanation for biased hybrid sterility and inviability. Evolution, 45, 262–267.
Gotoh, T, and Niijima, K. 1986. Characteristics and agents of abnormal sex ratio in two aphidophagous coccinellid species. In: Hodek, I (ed.) Ecology of the Aphidophaga, Academia, Prague, pp. 545–550.
Hackett, K J, Lynn, D E, Williamson, D L, Ginsberg, A S, and Whitcomb, R F. 1986. Cultivation of Drosophila sex ratio spiroplasma. Science, 232, 1253–1255.
Haig, D, and Grafen, A. 1992. Genetic scrambling as a defence against meiotic drive. J Theor Biol, 153, 531–538.
Hamilton, W D. 1967. Extraordinary sex ratios. Science, 156, 477–488.
Hamilton, W D. 1971. The geometry of the selfish herd. J Theor Biol, 31, 295–311.
Hawkes, O A M. 1920. Observations on the life history, biology and genetics of the ladybird beetle Adalia bipunctata. Proc Zool Soc, 33, 475–489.
Hodek, I. 1973. The Biology of the Coccinellidae. Academia, Prague.
Houck, M A, Clark, J B, Peterson, K R, and Kidwell, M G. 1991. Possible horizontal transfer of Drosophila genes by the mite Proctolaelaps regalis. Science, 253, 1125–1129.
Hu, H. 1979. Maternally inherited ‘sonless’ abnormal sex ratio condition in the ladybeetle Harmonia axyridis. Acta Genet Sin, 6, 296–304 (in Chinese).
Hurst, L D. 1991. The incidences and evolution of cytoplasmic male killers. Proc Roy Soc Lond B, 244, 91–99.
Hurst, L D, Godfray, H C J, and Harvey, P H. 1990. Antibiotics cure asexuality. Nature, 346, 510–511.
Hurst, L D, and Pomiankowski, A. 1991. Causes of sex ratio bias may account for unisexual sterility in hybrids: a new explanation for Haldane's rule and related phenomena. Genetics, 128, 841–858.
Karlin, S, and Lessard, S. 1986. Theoretical Studies on Sex Ratio Evolution. Princeton University Press, New Jersey.
Kearns, P W E, Tomlinson, I P M, Veltmann, C J, and O'Donald, P. 1991. Non-Random mating in the two spot ladybird, Adalia bipunctata. II Further tests for female mate preference. Heredity, 68, 385–389.
Lanier, G N, and Oliver, J H. 1966. Sex ratio condition: an unusual mechanism in bark beetles. Science, 153, 208–209.
Legrand, J J, Legrand-Hamelin, E, and Juci, P. 1987. Sex determination in Crustacea. Biol Rev, 62, 439–470.
Leslie, J F. 1984. A sex ratio condition in Oncopeltus fasciatus. J Hered, 75, 260–264.
Lewis, D. 1941. Male sterility in natural populations of hermaphroditic plants: The equilibrium between females and hermaphrodites to be expected with different types of inheritance. New Phytol, 40, 56–63.
Lus, Y Y. 1947a. Some rules of reproduction in populations of Adalia bipunctata: non-male strains in populations. Dokl Akad Nauk SSSR, 57, 951–954.
Lus, Y Y. 1947b. Some rules of reproduction in populations of Adalia bipunctata: heterozygosity of lethal alleles in populations. Dokl Akad Nauk SSSR, 57, 825–828.
Magni, G. 1952. Sex ratio in Drosophila bifaciata. First Lombardo Sci Lett, 85, 274–286.
Niijima, K, and Nakajima, K. 1981. Abnormal sex ratio in Menochilius sexmaculatus (Fabricius). Bull Fac Agric Tamagawa Univ, 21, 59–67. (in Japanese).
Randall, K, Majerus, M E N, and Forge, H. 1992. Characteristics for sex determination in British ladybirds Coleoptera: Coccinellidae. Entomologist, (in press).
Stouthamer, R, Luck, R F, and Hamilton, W D. 1990. Antibiotics cause parthenogenetic Trichogramma (Hymenoptera/Trichogrammatidae) to revert to sex. Proc Natl Acad Sci USA, 87, 2424–2427.
Taylor, D R. 1990. Evolutionary consequences of cytoplasmic sex ratio distorters. Evol Ecol, 4, 235–248.
Taylor, P D. 1981. Intra-and Inter-sex interaction as sex ratio determinants. Nature, 291, 64–66.
Trivers, R L, and Willard, D E. 1973. Natural selection of parental ability to vary the sex ratio of offspring. Science, 179, 90–92.
Uyenoyama, M K, and Feldman, M W. 1978. The genetics of sex ratio distortion by cytoplasmic infection under maternal and contagious transmission: an epidemiological study. Theor Pop Biol, 14, 471–497.
Werren, J H. 1980. Sex ratio adaptations to local mate competition in a parasitic wasp. Science, 208, 1157–1159.
Werren, J H. 1987. The coevolution of autosomal and cytoplasmic sex ratio factors. J Theor Biol, 124, 317–334.
Whyttle, T, Sandler, L M, Prout, T, and Perkins, D T. 1991. The genetics and evolutionary biology of meiotic drive. Discussion symposium on meiotic drive. Am Nat, 137, 281–456.
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Hurst, G., Majerus, M. & Walker, L. Cytoplasmic male killing elements in Adalia bipunctata (Linnaeus) (Coleoptera: Coccinellidae). Heredity 69, 84–91 (1992). https://doi.org/10.1038/hdy.1992.97
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