In total 14 isozyme gene loci were tested for segregation in Picea abies. Single tree segregation was not significantly heterogeneous. Pooled over all trees, segregation at the Idh-1 and Pgi-2 gene loci deviated significantly from the Mendelian expectation. Out of 91 two-locus combinations 57 could be analysed for linkage. The recombination frequencies were estimated employing the maximum likelihood and the Bayesian likelihood methods. Estimations of the recombination rates confirmed the close linkage group of Got-1:Pgi-2 in Pinaceae. Also Dia-2 appears to be linked to Pgi-2 and Got-1. Moderate linkage groups of Got-2:Skdh-1 and Lap-2:Pgi-2 were found but not in all of the investigated trees.
Adams, W T, and Joly, R J. 1980. Linkage relationships among twelve allozyme loci in loblolly pine. J Hered, 71, 33–40.
Allard, R W. 1983. Evidence for genetic restriction of recombination in the Lima bean. Genetics, 48, 1389–1395.
Barret, J W, Cheliak, W M, and Knowles, P. 1987. Variation at the PGI/AAT linkage group between populations of black spruce. Can J For Res, 17, 756–758
Bergmann, F. 1973. Genetische Untersuchungen bei Picea abies mit Hilfe der Isoenzym-Identifizierung. II. Genetische Kontrolle von Esterase- und Leucinaminopep-tidase-Isoenzymen im haploiden Endosperm ruhender Samen. Theor Appi Genet, 43, 222–225.
Bergmann, F. 1974. The genetics of some isoenzyme systems in spruce endosperm (Picea abies). Genetica, 6, 353–360.
Boyle, T J, and Morgenstern, E K. 1985. Inheritance and linkage relationships of some isozymes of black spruce in New Brunswick. Can J For Res 15, 992–996.
Cheliak, W M, Morgan, K, Dancik, B P, Strobeck, C, and Yeh, F C H. 1984. Segregation of allozymes in mega-gemetophytes of viable seed from a natural population of jack pine, Pinus banksiana Lamb. Theor Appl Genet, 69, 145–151.
Cheliak, W M, and Pitel, J A. 1985. Inheritance and linkage of allozymes in Larix laricina. Silvae Genet, 34, 142–148.
Cheliak, W M, Skroppa, T, and Pitel, J A. 1987. Genetics of the polycross. 1. Experimental results from Norway spruce. Theor Appl Genet, 73, 321–329
Conkle, M T. 1981. Isozyme variation and linkage in six conifer specie. In Proc. Symp. Isozymes of North American Forest Trees and Forest Insects. USDA For. Serv. Gen. Tech. Rep., PSW-48, pp. 11–17.
Conkle, M T, Hodgskiss, P D, Nunnally, L B, and Hunter, S C. 1982. Starch gel electrophoresis of conifer seeds: a laboratory manual. Gen Tech Rep PSW-64, Berkley, CA, USDA, p. 18.
Eckert, R R, Joly, R J, and Neale, D B. 1981. GenetlCS of isozyme variants and linkage relationships among allozyme loci in eastern white pine clones. Can J For Res, 11, 573–579.
Furnier, G R, Knowles, P, Aleksiuk, M A, and Dancik, B P. 1986. Inheritance and linkage in allozymes in seed tissue of whitebark pine. Can J Genet CytoL, 28, 601–604.
Fisher, R A. 1937. The design of experiments. Oliver and Boyd, Edinburgh.
Frei, O M, Stuber, C W, and Goodman, M M. 1986. Use of allozymes as genetic markers for predicting performance in maize single cross hybrids. Crop 5c 26, 37–41.
Geburek, T H, and Wang, Q-H. Inheritance of isozyme polymorphisms and their linkage relations in Chinese fir (Cun-ninghamia lanceolata (Hook)). Hereditas, (submitted).
Geburek, T H, Scholz, F, Knabe, W, and Vornweg, A. 1987. Genetic studies by isozyme gene loci on tolerance and sensitivity in an air polluted Pinus sylvestris field trial. Silvae Genet, 36, 49–53.
Guries, R P, Friedman, S T, and Ledig, F T. 1978. A megagametophyte analysis of genetic linkage in pitch pine (Pinus rigida Mill.). Heredity, 40, 309–314.
Grell, R. 1966. The meiotic origin of temperature induced crossovers in Drosophila melanogaster females. Genetics, 54, 411–421.
Jeffreys, H. 1961. Theory of Probability. Clarendon Press, Oxford.
King, J N, and Dancik, B P. 1983. Inheritance and linkage of isozymes in white spruce (Picea glauca). Can J Genet CytoL, 25, 430–436.
Kosambi, B D. 1944. The estimation of map distance from recombination values. Ann Eugen London, 12, 172–175.
Lundkvist, K. 1974. Inheritance of leucine aminopeptidase isozymes in Picea abies K. Hereditas, 76, 91–96.
Lundkvist, K. 1975. Inheritance of acid phosphatase isozymes in Picea abies K. Hereditas, 79, 221–226.
Lundkvist, K. 1979. Allozyme frequency distributions in four Swedish populations of Norway spruce (Picea abies K.). 1. Estimations of genetic variation within and among populations, genetic linkage and a mating system parameter. Hereditas, 90, 127–143.
Mather, K. 1951. The Measurement of Linkage in Heredity, Methuen & Co. London.
Muller-Starck, G, and Liu, Y-Q. Genetics of Cunninghamia lanceolata Hook. 1. Genetic analysis. Silvae Genet, (in press).
Moran, G F, Bell, J C, and Hilliker, A J. 1983. Greater meiotic recombination in male vs. female gametes in Pinus radiat a. J Her ed, 74, 62.
Muona, O, Yazdani, R, and Lindquist, G. 1987. Analysis of linkage in Picea abies. Hereditas, 106, 31–36.
Nordheim, E V, O'Malley, D M, and Guries, R P. 1983. Estimation of recombination frequency in genetic linkage studies. Theor Appl Genet, 66, 313–321.
O'Malley, D M, Guries, R P, and Nordheim, E V. 1986. Linkage analysis for 18 enzyme loci in Pinus rigida Mill. Theor Appl Genet, 72, 530–535.
Poulsen, H D, Simonsen, V, and Wellendorf, H. 1983. The inheritance of six isozymes in Norway spruce (Picea abies (L.) Karst.). Forest Tree Improv, 16, 12–33.
Rao, C R. 1973. Linear Statistical Inference and Its Application. John Wiley and Sons, New York.
Rudin, D, and Ekberg, I. 1978. Linkage studies in Pint/5 sylvestris L. using macro gametophyte allozymes. Silvae Genet, 27, 1–12.
Shiraishi, S. 1988. Linkage relationships among allozyme loci in Japanese black pine, Pinus thunbergii Pari. Silvae Genet, 37, 60–66.
Strauss, S H, and Conkle, M T. 1986. Segregation, linkage, and diversity of allozymes in knobcone pine. Theor Appl Genet, 72, 483–493.
Tigerstedt, P M A. 1973. Studies on isozyme variation in marginal and central populations of Picea abies. Hereditas, 75, 47–60.
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Geburek, T., von Wuehlisch, G. Linkage analysis of isozyme gene loci in Picea abies (L.) Karst.. Heredity 62, 185–191 (1989). https://doi.org/10.1038/hdy.1989.27
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