Abstract
Results have been obtained which corroborate those of Pandey and Patchell (Molec. Gen. Genet., 186, 305, 1982) in demonstrating that genetic material from irradiated semen is incorporated into the embryo and expressed, albeit at rather a low rate, and is subsequently transmitted to progeny of the transfected birds. The method provides a technically straightforward means of transferring genetic material where rapid and reliable means of detecting the transferred gene exist. An advantage of the method is that regulatory regions are likely to be carried with the transferred gene but there is equally a disadvantage in the simultaneous transfer of unwanted material.
Similar content being viewed by others
Article PDF
References
Astrin, S M, Robinson, H L, Crittenden, L B, Buss, E G, Wyban, J, and Hayward, W S. 1979. Ten genetic loci in the chicken that contain structural genes for endogenous virus. Cold Spring Harbor Symp Quant Biol, 44, 1105–1109.
Bloom, S E, Cole, R K, and Bacon, L D. 1978. Chromosomal localization of the major histocompatibility (B) locus in the chicken. Poult Sci, 57, 1119.
Briles, W E, Bumstead, N, Ewart, D L, Gilmour, D G, Gogusev, J, Hala, K, Koch, C, Longenecker, B M, Nordskog, A W, Pink, J R L, Schierman, L W, Simonsen, M, Toivanen, A, Toivanen, P, Vaino, O, and Wick, G. 1982. Nomenclature for chicken major histocompatibility complex. Immunogenetics, 15, 441–447.
Delorbe, W J, Luaw, P A, Goodman, H M, Varmus, H E, and Bishop, J M. 1980. Molecular cloning and characterization of avian sarcoma virus circular DNA molecules. J Virol, 36, 50–61.
Dodgson, J B, Strommer, J, and Engel, J D. 1979. Isolation of the chicken β-globin gene and a linked embryonic β-like globin gene from a chicken DNA recombinant library. Cell, 17, 879–887.
Hammer, R E, Pursel, V G, Rexroad, C E, Wall, R J, Bolt, D J, Ebert, K M, Palmiter, R D, Brinster, R L. 1985. Production of transgenic rabbits, sheep and pigs by microinjection. Nature, 315, 680–683.
Hughes, S H, Payvar, F, Spector, D, Schimke, R T, Robinson, H L, Payne, G S, Bishop, J M, and Var Mus, H E. 1979. Heterogeneity of genetic loci in chickens: analysis of endogenous viral and normal genes by cleavage of DNA with restriction endonucleases. Cell, 18, 347–359.
Humphries, E H, Danhof, M L, and Hlozanek, I. 1984. Characterization of endogenous viral loci in five lines of White Leghorn chickens. Virology, 135, 125–138.
Palmiter, R D, Brinster, R L, Hammer, R E, Trumbane, M E, Rosenfeld, M G, Birnberg, N C, and Evans, R M. 1982. Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes. Nature, 300, 611–615.
Pandey, K K, Patchell, M R. 1982. Genetic transformation in chicken by the use of irradiated male gametes. Mol Gen Genet, 186, 305–308.
Plachy, J, Hala, K, and Vilhelmova, M. 1979. A spontaneous chicken chimaera with serologically and morphologically different types of erythrocytes. Folia Biol Praha, 25, 389–391.
Report. (1983–84). Houghton Poultry Research Station, pp. 21–22.
Rigby, P W J, Dieckmann, M, Rhodes, C, and Berg, P. 1977. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase 1. J Mol Biol, 113, 237–251.
Rosenthal, P N, Robinson, H L, Robinson, W S, Hanafusa, T, and Hanafusa, H. 1971. DNA in uninfected and virus-infected cells complementary to avian tumor virus RNA. Proc Natn Acad Sci, USA, 68, 2336–2340.
Southern, E M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol, 98, 503–517.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bumstead, N., Messer, L., Freeman, B. et al. Genetic transformation of chickens using irradiated male gametes. Heredity 58, 25–30 (1987). https://doi.org/10.1038/hdy.1987.4
Received:
Issue Date:
DOI: https://doi.org/10.1038/hdy.1987.4
This article is cited by
-
Transgenesis in chickens
Transgenic Research (1993)
-
Production of transgenic birds
Experientia (1991)
-
The improbability of irradiated spermatozoa as gene transfer vectors in chickens
Theoretical and Applied Genetics (1990)