Summary
Using flower colour markers that express themselves only in the epidermis, in a stable periclinal chimaera of Petunia it was demonstrated that regenerants from callus grown from pedicels and leaves have the genotype of the epidermal cell layer. When a plant carrying an unstable allele of the gene An1 is tested in this way, occasional branches with a mutant flower colour type appear to have the mutant genotype only in the epidermis.
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Bino, R., Bianchi, F. & Wijsman, H. Periclinal chimaerism in Petunia demonstrated by regeneration of plants from the mutated epidermal layer. Heredity 52, 437–441 (1984). https://doi.org/10.1038/hdy.1984.52
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DOI: https://doi.org/10.1038/hdy.1984.52
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