Summary
M1 plants derived from crosses using pollen irradiated with 20 Krad of γ-rays were selfed and backcrossed reciprocally to N rustica, V27. A cytogenetical examination of these progenies revealed the persistence of considerable aneuploidy and structural aberrations. It proved possible to detect several aberrant chromosomes in the M1 parents and observe their transmission rates to the offspring. Analysis indicated some post-meiotic selection against these damaged, paternal chromosomes. The intensity of this selection, however, is insufficient to account for the maternal trend previously reported for the M2 generation in N. rustica. The preferred interpretation is that the mutational damage may lead to a loss in vigour during the M2 and equivalent generations.
Similar content being viewed by others
Article PDF
References
Caligari, P D S, Ingram, N R, and Jinks, J L. 1981. Gene transfer in Nicotiana rustica by means of irradiated pollen. I. Unselected progenies. Heredity, 47, 17–26.
Pandey, K K. 1975. Transformation, incompatibility and plant improvement. Incompatibility Newsletter, 6, 91–121.
Pandey, K K. 1980. Further evidence for egg transformation in Nicotiana. Heredity, 45, 17–31.
Pandey, K K. 1983. Evidence for genetic transfer by the use of sublethally irradiated pollen in Zea mays and theory of occurrence by chromosome repair through somatic recombination and gene conversion. Molecular and General Genetics, 191, 358–365.
Powell, W, Caligari, P D S, and Hayter, A M. 1983. The use of pollen irradiation in plant breeding. Theoretical and Applied Genetics, 65, 73–76.
Sears, E R. 1954. The aneuploids of common wheat. Missouri Agricultural Experiment Station Research Bulletin, 572, 1–59.
Snape, J W, Parker, B B, Simpson, E, Ainsworth, C C, Payne, P I, and Law, C N. 1983. The use of irradiated pollen for differential gene transfer in wheat (Triticum aestivum). Theoretical and Applied Genetics, 65, 103–111.
Werner, C P, Dunkin, I M, Cornish, M A, and Jones, G H. 1984. Gene transfer in Nicotiana rustica by means of irradiated pollen. II. Cytogenetical consequences. Heredity, 52, 113–119.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Werner, C., Cornish, M. Gene transfer in Nicotiana rustica by means of irradiated pollen. III. Cytogenetical consequences in the second generation. Heredity 53, 545–551 (1984). https://doi.org/10.1038/hdy.1984.114
Received:
Issue Date:
DOI: https://doi.org/10.1038/hdy.1984.114
This article is cited by
-
Pollination with irradiated pollen in rice—Oryza sativa L. I. First (M1) generation
Heredity (1989)
-
Gene transfer through the use of sublethally irradiated pollen: the theory of chromosome repair and possible implication of DNA repair enzymes
Heredity (1986)
-
Gene transfer in Nicotiana rustica by means of irradiated pollen IV. Qualitative characters
Heredity (1985)
-
Gene transfer in Nicotiana rustica by means of irradiated pollen V. Quantitative characters
Heredity (1985)