Figure 6

From: siRNA silencing of keratinocyte-specific GFP expression in a transgenic mouse skin model

Figure 6

Intravital imaging of siRNA-mediated hMGFP inhibition in mouse footpad skin using DAC fluorescence microscopy. Mouse footpads were treated daily for 18 days with an irrelevant siRNA (K6a_513a.12) (left) or CBL3 siRNA (right) as described in Materials and methods. The images show a side view of a 3D volume rendering of 80 × 250 μm en face images through a depth of 100 μm. Images were taken at day 19 using the same gain setting for both footpads. The hMGFP signal strongly accumulates in the stratum corneum (SC) of the control footpad, and cytoplasmic granules can be detected in the granular layer (G). Strong inhibition of hMGFP signal is observed in the CBL3 siRNA-treated footpad for both SC and G. The 2D image stack videos of these 3D images are provided as Supplementary material.