Figure 4

From: siRNA silencing of keratinocyte-specific GFP expression in a transgenic mouse skin model

Figure 4

CBL3 siRNA potently and specifically inhibits pre-existing hMGFP expression in mouse epidermis. Transgenic hMGFP mouse footpads were treated daily by intradermal injection of 60 μg of CBL3 or non-specific control K6a_513a.12 siRNAs for 14 days. The mice were killed and frozen skin sections (10 μm) prepared. The hMGFP expression (or lack thereof) was visualized by fluorescence microscopy. (a and b) Cross sections from mouse footpad intradermally injected with the non-specific siRNA (K6a_513a.12) (a) or CBL3 (b) siRNA. (c and d) Same conditions as in panels a and b, but in combination with 5 μg of pUC19. Scale bar is 20 μm. Nuclei are visualized by DAPI stain (blue).