Figure 2

From: siRNA silencing of keratinocyte-specific GFP expression in a transgenic mouse skin model

Figure 2

In situ hybridization of hMGFP/CBL mRNA in mouse epidermis. Tg CBL/hMGFP mouse footpad skin was fixed in paraformaldehyde and frozen in OCT, as described in Materials and methods. Sections (10 μm) were prepared and fixed again before hybridization with hMGFP and K14 Dig-labeled riboprobes. Washed sections were incubated with anti-Dig antibodies and subsequently developed with NBT and BCIP. Stratum corneum (SC), granular layer (G) and basal layer (B). The scale bar is 50 μm.