Figure 1

From: siRNA silencing of keratinocyte-specific GFP expression in a transgenic mouse skin model

Figure 1

Transgenic mouse model in which hMGFP reporter is expressed in the epidermis. (a) Schematic of hMGFP reporter transgene. A transgenic mouse expressing Cre recombinase driven by the keratinocyte-specific K14 promoter was crossed with a silenced reporter mouse, as described in Materials and methods, resulting in a reporter mouse in which hMGFP expression is driven by a modified CAG promoter. (b and c) The hMGFP expression in mouse footpad skin sections is confined to the epidermis. Skin vertical cross sections (10 μm) were obtained from positive (b) and negative (c) littermates and hMGFP signal detected by fluorescent microscopy. The majority of hMGFP expression is localized in the granular layer and stratum corneum. The scale bar is 20 μm. Nuclei are visualized by DAPI stain (blue). Left panels show bright field overlay.