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Progress and Prospects: The design and production of plasmid vectors

Gene Therapy volume 16, pages 165–171 (2009)Cite this article

Abstract

Plasmid DNA (pDNA) expression vectors are fundamental to all forms of non-viral gene transfer. In this review, we discuss principles of pDNA design and production including the impact of bacterially derived sequences on transgene expression and minicircle approaches to minimize their effects. The impact of inclusion of DNA elements such as scaffold matrix attachment regions (S/MARs), transcription factor (TF)-binding sites and tissue-specific promoters are described. The benefits of eliminating CG dinucleotides (CpGs) from the pDNA are also considered.

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Authors and Affiliations

  1. Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford, UK

    D R Gill, I A Pringle & S C Hyde

  2. The UK Cystic Fibrosis Gene Therapy Consortium,

    D R Gill, I A Pringle & S C Hyde

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  1. D R Gill
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  2. I A Pringle
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  3. S C Hyde
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Correspondence to S C Hyde.

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Gill, D., Pringle, I. & Hyde, S. Progress and Prospects: The design and production of plasmid vectors. Gene Ther 16, 165–171 (2009). https://doi.org/10.1038/gt.2008.183

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