Table 1 Sequence of PCR primers

From: Alternative splicing directs two IL-20R2 isoforms and is responsible for the incomplete gene knockout via the exon I ablation

Primer name Sequence Application
UW27 5′-CTGGAACTCTTACAGGCCAC-3′ Genotyping
UW28 5′-CTACCAGGTAAGGGAGGAGC-3′ Genotyping
UW69 5′-CTTCTCACTGCCCTGTATGCTAAG-3′ Genotyping
hl51 5′-CCTGAAAACTTTGCCCCCTCCATA-3′ Genotyping
P1 5′-GTCTGGACAAGTCCGTTCATG-3′ RT-PCR/qPCR
P2 5′-CTCTGTACAGTCAACCAACATG-3′ RT-PCR
P3 5′-CTAGGTGCACCGGAATGTC-3′ RT-PCR
P4 5′-CACTGTTGATGAACCTGTAAG-3′ RT-PCR
P5 5′-CAGAAACACAACCCACCC -3′ RT-PCR
P6 5′-GCTTCATGTTGGTTGACTG-3′ RT-PCR
P7 5′-CAAAAGGCCCACAAGATAGG-3′ RT-PCR
P8 5′-CATAGTATAGTAATTTGAGAGG-3′ RT-PCR
P9 5′-GCTTCATGTTGGTTGACTGTAC-3′ q-PCR
P10 5′-GGTCAGCTTCTCTACTTAATTTCG-3′ q-PCR
RPL-19-F 5′-GAAGGTCAAAGGGAATGTGTTCA-3′ RT-PCR/qPCR
RPL-19-R 5′-CCTTGTCTGCCTTCAGCTTGT-3′ RT-PCR/qPCR
pQE-F 5′-AGCAGATAACAATTTCACACAG-3′ Sequencing
pQE30-R 5′-TTCTGAGGTCATTACTGGATC-3′ Sequencing
GSP1 5′-GCTCTCGTACTCCCCCTGATACTCCAC-3′ 5′ RACE
GSP2 5′-GAGATGCTTCATGTTGGTTGACTGTAC-3′ 5′ RACE
CGHG9C 5′-CGAAGCTTGGGGGGGGGC-3′ 5′ RACE
CGHG9A 5′-CGAAGCTTGGGGGGGGGA-3′ 5′ RACE
CGHG9T 5′-CGAAGCTTGGGGGGGGGT-3′ 5′ RACE
  1. Abbreviations: qPCR, quantitative PCR; 5′ RACE, rapid amplification of cDNA ends; RT-PCR, reverse transcription PCR. Primers were written from 5′_to 3′_ of the source sequences. Reference sequence were mouse IL-20R2 DNA (NC_000075.6); mouse IL-20R2308 mRNA (NM_001033543); mouse IL-20R2302 mRNA (GenBank Accession No.: KT878835); and the housekeeping gene RPL-19 (ribosomal protein L19) (XM_006532610).