The uncovering of ESE-1 in human neutrophils: implication of its role in neutrophil function and survival

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Abstract

Epithelium-specific Ets transcription factor 1 (ESE-1) is a member of the E26 transformation-specific family of transcription factors that has an epithelial-restricted constitutive expression but is induced by inflammatory stimuli in non-epithelial cells. Here we report that ESE-1 is constitutively expressed in human, but not in murine, neutrophils and that ESE-1 is modestly upregulated in septic patient neutrophils. In normal human neutrophils, ESE-1 was detected at both RNA and protein levels but was found to be an unstable nuclear protein ex vivo. ESE-1 transcription was also induced during all-trans retinoic acid-mediated HL-60 differentiation, a human promyelocytic cell line often used as an in vitro model of human neutrophils. Elf3−/− mice had normal neutrophils but a reduced number of circulating B-lymphocytes. These findings indicate a potential role of ESE-1 in regulating human neutrophil differentiation and function, and that it has different roles in the immune system of different species.

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Acknowledgements

We thank Arthur Gutierrez-Hartmann (University of Colorado) for his generous gift of the pEGFP-C3-empty and pEGFP-ESE-1 vectors. This study was funded by Canadian Institute for Health Research (CIHR) grant (MOP 125882) to Jim Hu. Chan Mi Lee is a recipient of Ontario Graduate Scholarship (OGS), Peterborough K.M. Hunter Studentship and SickKids Restracomp Studentship. We acknowledge that no authors have conflicting financial interests.

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Correspondence to J Hu.

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