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Interleukin-10 receptor-1 expression in monocyte-derived antigen-presenting cell populations: dendritic cells partially escape from IL-10's inhibitory mechanisms

Genes & Immunity volume 16, pages 8–14 (2015)Cite this article

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A Corrigendum to this article was published on 23 July 2015

Abstract

Interleukin (IL)-10 is an important immunoregulatory cytokine that mediates its effects via a transmembrane receptor complex consisting of two different chains, IL-10R1 and IL-10R2. While IL-10R2 is ubiquitously expressed and does not bind IL-10 primarily, the expression of IL-10R1 determines cellular responsiveness. However, the current knowledge about the expression and regulation of IL-10R1 is still limited. Here we analyzed the expression of IL-10R1 on monocytic cells and demonstrated that human blood monocytes carried about 720 IL-10-binding sites on their surface. Compared with lymphocytes and various tissue cells and tissues, blood monocytes expressed the highest IL-10R1 levels. The in vitro differentiation of these cells into macrophages provoked a further increase of IL-10R1 surface expression. In contrast, their differentiation into myeloid dendritic cells (mDCs) resulted in reduced surface IL-10R1 levels. The different IL-10R1 levels expressed by monocyte-derived antigen-presenting cell populations were reflected in their different responsiveness toward IL-10. Importantly, also in vivo developed immature macrophages and mDCs showed different IL-10 sensitivity. These data suggest that, compared with monocytes and macrophages, mDCs partially escape from IL-10's inhibitory mechanisms by downregulating IL-10R1.

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Acknowledgements

We thank Antje Haeußler-Quade, Christa Liebenthal and Ricardo Wieseke for excellent technical help. Special thanks is due to Dr Nigel EA Crompton for his continuous courtesy to read the manuscript and to correct the English syntax.

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Author notes

  1. S H von Lanzenauer and K Wolk: These authors contributed equally to this work.

Authors and Affiliations

  1. Institute of Medical Immunology, University Hospital Charité, Berlin, Germany

    S H von Lanzenauer, C Höflich, U Reineke & H-D Volk

  2. Interdisciplinary Group of Molecular Immunopathology, Dermatology/Medical Immunology, University Hospital Charité, Berlin, Germany

    K Wolk, S Kunz & R Sabat

  3. Research Center Immunosciences, University Hospital Charité, Berlin, Germany

    K Wolk & R Sabat

  4. Bayer Pharma AG, Berlin, Germany

    B H Grünberg, W-D Döcke & K Asadullah

  5. Department of Dermatology and Allergy, University Hospital Charité, Berlin, Germany

    W Sterry

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  1. S H von Lanzenauer
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Correspondence to R Sabat.

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von Lanzenauer, S., Wolk, K., Höflich, C. et al. Interleukin-10 receptor-1 expression in monocyte-derived antigen-presenting cell populations: dendritic cells partially escape from IL-10's inhibitory mechanisms. Genes Immun 16, 8–14 (2015). https://doi.org/10.1038/gene.2014.69

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