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Lack of association between genetic variation in G-protein-coupled receptor for asthma susceptibility and childhood asthma and atopy

Genes & Immunity volume 9, pages 224–230 (2008)Cite this article

Abstract

G-protein-coupled receptor for asthma susceptibility (GPRA or GPR154) was identified as an asthma and atopy candidate gene by positional cloning. Some subsequent studies suggest associations of GPRA single nucleotide polymorphisms (SNPs) and haplotypes with asthma or atopy susceptibility. However, the associated SNPs or haplotypes vary among studies. The role of GPRA genetic variation in asthma and atopy remains unsolved. Published data on GRPA variants and asthma come exclusively from Caucasian and Asian populations. We examined whether GPRA SNPs and haplotypes are associated with asthma and atopy in a Mexican population. We genotyped and analyzed 27 GPRA SNPs in 589 nuclear families consisting of asthmatic children aged 4–17 years of age and their parents in Mexico City. Atopy was determined by skin prick tests to 25 aeroallergens. The 27 SNPs examined provided excellent coverage of the GPRA gene. GPRA SNPs and haplotypes were not associated with childhood asthma and the degree of atopy to aeroallergens in a Mexican population. Our review of studies of GPRA variants in relation to asthma phenotypes shows considerable heterogeneity. Accordingly, our results suggest that GPRA variants are not an important contributor to childhood asthma and atopy susceptibility in a Mexican population.

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Acknowledgements

We thank the children and parents who participated in this study; Grace Chiu (Westat, Research Triangle Park, NC, USA) for data management and analysis; Susan Baker, Sarah Lein and Sheri McReynolds (Coda Research Inc., Research Triangle Park, NC, USA) for specimen handling; and Dulce Ramirez for participation in the fieldwork. Subject enrollment and the current work were supported by the Division of Intramural Research, National Institute of Environmental Health Sciences (Z01 ES49019), National Institutes of Health, Department of Health and Human Services, USA. Subject enrollment was supported in part by the National Council of Science and Technology (grant 26206-M), Mexico. Dr Romieu is supported by the National Center for Environmental Health at the Centers for Disease Control.

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Authors and Affiliations

  1. Division of Intramural Research, Department of Health and Human Services, Laboratory of Respiratory Biology, National Institute of Environmental Health Sciences, National Institutes of Health, NC, USA

    H Wu, H Li & S J London

  2. National Institute of Public Health, Cuernavaca, Morelos, Mexico

    I Romieu

  3. Hospital Infantil de Mexico Federico Gomez, Mexico City, Mexico

    J-J Sienra-Monge & B E del Rio-Navarro

  4. Core Genotyping Facility, Advanced Technology Center, National Cancer Institute, Gaithersburg, MD, USA

    L Burdett, J Yuenger & S J Chanock

  5. Division of Intramural Research, Department of Health and Human Services, Epidemiology Branch, National Institute of Environmental Health Sciences, National Institutes of Health, NC, USA

    S J London

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  1. H Wu
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Correspondence to S J London.

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The authors declare that they have no competing financial interests.

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Wu, H., Romieu, I., Sienra-Monge, JJ. et al. Lack of association between genetic variation in G-protein-coupled receptor for asthma susceptibility and childhood asthma and atopy. Genes Immun 9, 224–230 (2008). https://doi.org/10.1038/gene.2008.8

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