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Defining a transcriptional fingerprint of murine splenic B-cell development

Genes & Immunity volume 9, pages 706–720 (2008)Cite this article

Abstract

B-cell development occurs in a stepwise fashion that can be followed by the expression of B cell-specific surface markers. In this study, we wished to identify proteins that could contribute to the changes in expression of such markers. By using RNA from freshly isolated B220+ cells, we hoped to reduce the effect of artifacts that occur during the isolation and amplification steps necessary to use flow cytometry analysis-sorted subsets in microarray experiments. Analyses comparing expression patterns from B220+ 2-week bone marrow (pro-B, pre-B, immature B cells), 2-week spleen (predominantly transitional cells) and 8-week spleen (mainly mature B cells) yielded hundreds of genes. We also examined the B cell-activating factor (BAFF)-dependent effects on immature splenic B cells by comparing expression patterns in the spleen between 2-week A/J vs 2-week A/WySnJ mice, which lack functional BAFF receptor signaling. Genes that showed the expression differences between spleen and bone marrow samples were then analyzed through quantitative PCR on B-cell subsets isolated using two different sorting protocols. A comparison of the results from our study with the results from other analyses showed not only some overlap of preferentially expressed genes but also an expansion of other genes potentially involved in B-cell development.

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Acknowledgements

We thank the University of Utah FACS and Oligonucleotide Core Facilities for their assistance. We also thank Dr Peter Jensen for providing the C2ta-deficient animals. We sincerely acknowledge the technical help and suggestions from Dr Wayne Green for assistance in the isolation of B-cell subsets. Finally, we also thank all the members of our laboratories for their insightful critiques of this work.

This work was supported by grants from the National Institute of Allergy and Infectious Diseases (AI-24158, JHW: AI-32223, JJW). The content is solely the responsibility of the authors and does not necessarily represent the official views of the Institute of Allergy and Infectious Diseases or the National Institutes of Health.

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  1. Division of Cell Biology and Immunology, Department of Pathology, University of Utah School of Medicine, Salt Lake City, UT, USA

    I Debnath, K M Roundy, J J Weis & J H Weis

  2. Department of Human Genetics, University of Utah School of Medicine, Salt Lake City, UT, USA

    D M Dunn & R B Weiss

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  1. I Debnath
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Correspondence to J H Weis.

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Supplementary Information accompanies the paper on Genes and Immunity website (http://www.nature.com/gene)

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Debnath, I., Roundy, K., Dunn, D. et al. Defining a transcriptional fingerprint of murine splenic B-cell development. Genes Immun 9, 706–720 (2008). https://doi.org/10.1038/gene.2008.70

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