Abstract
Purpose The influence of subconjunctival steroid injection on blood glucose concentration was investigated in rats with streptozotocin (STZ)-induced diabetes.
Methods Adult male Wistar rats with STZ-induced diabetes (n = 10) and normal controls (n = 10) received subconjunctival injections of 0.1 ml of 0.4% dexamethasone or saline. Blood glucose concentrations were measured before and 3, 6, 12, 18 and 24 h after treatment.
Results In the STZ group, subconjunctival injection of steroids (p = 0.0013) or saline (p = 0.0037) significantly increased the blood glucose level 3 h after treatment. In the control group, the blood glucose concentration was not elevated by subconjunctival injection. In both STZ and control groups, the blood glucose concentration was significantly higher after steroid injection than after saline injection throughout the 24 h study period.
Conclusions Subconjunctival steroid injection induced a significant blood glucose increase in both diabetic and control rats. For diabetic rats, the subconjunctival injection itself constituted stress that resulted in glucose elevation.
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Avasthi P, Bhatia R . Role of subconjunctival injection of steroid in extracapsular extraction of lens. Int Surg 1969;51:537–9.
Buxton J, Smith D, Brownstein S . Cataract extraction and subconjunctival repository corticosteroids. Ann Ophthalmol 1971;3:1871–6.
Corbett M, Hingorani M, Boulton J, et al. Subconjunctival betamethasone is of benefit after cataract surgery. Eye 1993;7:744–8.
Learning D . Practice styles and preferences of ASCRS members: 1998 survey. J Cataract Refract Surg 1999;25:851–9.
Oshika T, Masuda K, Majima Y, et al. Current trends in cataract and refractive surgery in Japan: 1995 survey. Jpn J Ophthalmol 1996;40:419–33.
Tsuji A, Tamai I, Sasaki K . Hydrolysis of prednisolone succinate by esterase in rabbit ocular tissue. Ophthalmic Res 1987;19:322–9.
Weijtens O, Feron E, Schoemaker R, et al. High concentration of dexamethasone in aqueous and vitreous after subconjunctival injection. Am J Ophthalmol 1999;128:192–7.
Delaunay F, Khan A, Cintra A, et al. Pancreatic beta cells are important targets for the diabetogenic effects of glucocorticoids. J Clin Invest 1997;100:2094–8.
Wajngot A, Khan A, Giacca A, et al. Dexamethasone increases glucose cycling, but not glucose production, in healthy subjects. Am J Physiol 1990;259:E626–32.
Deibert D, DeFronzo R . Epinephrine-induced insulin resistance in man. J Clin Invest 1980;65:717–21.
Rizza R, Cryer P, Haymond M, et al. Adrenergic mechanisms for the effects of epinephrine on glucose production and clearance in man. J Clin Invest 1980;65:682–9.
Chiasson J, Shikama H, Chu D, et al. Inhibitory effect of epinephrine on insulin-stimulated glucose uptake by skeletal muscle. J Clin Invest 1981;68:706–13.
Halter J, Beard J, Porte DJ . Islet function and stress hyperglycemia: plasma glucose and epinephrine interaction. Am J Physiol 1984;247:E47–52.
Shah S, McHugh J, Spalton D . The effects of subconjunctival betamethasone on the blood aqueous barrier following cataract surgery: a double-blind randomized prospective study. Br J Ophthalmol 1992;76:475–8.
Schmitt K, Hessemer V . Ist eine subkonjunktivale Steroidgabe zusatzlich zur Lokaltherapie nach Kataraktoperationen notwendig? Ophthalmologe 1995;92:303–6.
Fukushima H, Kato S, Kaiya T, et al. Effect of subconjunctival corticosteroid immediately after cataract surgery in diabetic patients [in Japanese]. Jpn J Clin Ophthalmol 1999;53:2001–4.
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Fukushima, H., Kato, S., Hayashi, Y. et al. Influence of subconjunctival steroid injection on blood glucose profile in diabetic rats. Eye 15, 326–328 (2001). https://doi.org/10.1038/eye.2001.104
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DOI: https://doi.org/10.1038/eye.2001.104