Abstract
Purpose To investigate the effects of exogenous and endogenous nitric oxide (NO) on the proliferation of human retina pigment epithelial (RPE) cells.
Methods We stimulated cultured human RPE cells with 3-morphosydnonimine (SIN-I) to analyse the effect of exogenous NO. Incubation with a cytokine cocktail (interleukin 1-β) + interferon γ + tumour necrosis factor α) plus lipopolysaccharide (LPS) induced cells to synthesise NO endogenously. The cultures were then incubated for 48 h, after which the cells were stained with crystal violet and absorbance at 550 nm was measured spectrophotometrically.
Results SIN-I inhibited human RPE cell proliferation, while haemoglobin, an NO inhibitor, almost completely blocked the inhibitory effect. On the other hand, treatment with the cytokine cocktail plus LPS did not inhibit RPE cell proliferation.
Conclusion These findings confirm that exogenous NO inhibits human RPE cell proliferation, while endogenous NO has no such blocking effect.
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This work was supported by Köin Fortune, Deutsche Forschungsgemeinschaft (Es 82/5-1, He 840/6-2) and Turkish Scientific and Technical Research Council
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Yilmaz, G., Esser, P., Kociek, N. et al. Effect of nitric oxide on proliferation of human retina pigment epithelial cells. Eye 14, 899–902 (2000). https://doi.org/10.1038/eye.2000.245
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DOI: https://doi.org/10.1038/eye.2000.245