Abstract
Purpose To assess the level of contamination of full-thickness skin grafts stored with or without an antibiotic cover.
Methods Full-thickness skin grafts were harvested from 40 bilateral upper lid blepharoplasties. Before surgery the face was sterilised, the head of the patient was packed with sterile, single-use surgical drapes and the whole face was left exposed. The harvested full-thickness skin grafts were conserved in sterile containers at 4 °C for 6 days, rolled in gauze moistened with either 4 ml of sterile saline solution (group I) or with 4 ml of gentamicin solution (2 mg/ml) (group II). The degree of contamination, expressed in colony forming units (CFU), was evaluated on days 2, 3, 4, 5 and 6. Identification of the microorganisms was done to species level following standard procedures and commercial methods.
Results In group I 2 grafts (5%) were negative during the whole observation period while the other 38 grafts (95%) presented a degree of contamination ranging from 102 to 104 CFU. Microorganisms isolated were: Staphylococcus epidermidis (24 cases), Staphylococcus aureus (5 cases), Staphylococcus saprophyticus (2 cases), Pseudomonas aeruginosa (4 cases), Serratia liquefaciens (1 case) and Klebsiella oxytoca (2 cases). In group II, 26 grafts (65%) were negative during the whole observation time while in 14 cases (35%) a few colonies (3 to 6) of Candida albicans were isolated on day 2 and remained constant in number for the whole observation time.
Conclusions The storage of full-thickness skin graft with an antibiotic cover is more reliable than the storage of full-thickness skin graft without an antibiotic cover.
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References
Collin JRO . A manual of systematic eyelid surgery, 2nd ed. Edinburgh: Churchill Livingstone, 1989:150–2.
Mustarde JC . Repair and reconstruction in the orbital region, 3rd ed. Edinburgh: Churchill Livingstone, 1991:539.
Tyers AG, Collin JRO . Colour atlas of ophthalmic plastic surgery. Edinburgh: Churchill Livingstone, 1995:32.
Farmer JJ III, Kelly MT . Enterobacteriaceae. In: Balows A, Hausler WJ Jr, Herrmann KL, Isenberg HD, Shadomy HJ, editors. Manual of clinical microbiology, 5th ed. Washington, DC: American Society for Microbiology, 1991:360–83.
Kloos WE, Lambe DW Jr. Staphylococcus. In: Balows A, Hausler WJ Jr, Herrmann KL, Isenberg HD, Shadomy HJ, editors. Manual of clinical microbiology, 5th ed. Washington DC: American Society for Microbiology, 1991:222–37.
Amies DR . Orbital cellulitis. J Laryngol Otol 1974;88:559–64.
Jarret W, Gutman F . Ocular complication of infection in the paranasal sinuses. Arch Ophthalmol 1969;81:683–8.
Von Noorden GK . Orbital cellulitis following extraocular muscle surgery. Am J Ophthalmol 1972;74:627–9.
Walters EC, Wallar PH, Hiles DA, Michaels RH . Acute orbital cellulitis. Arch Ophthalmol 1976;94:785–8.
Artenstein AW, Cross AS . Local and disseminated disease caused by Pseudomonas aeruginosa. In: Campa M, Bendinelli M, Friedman H, editors. Pseudomonas aeruginosa as an opportunistic pathogen. New York: Plenum Press, 1993:223–44.
Rosenoff SH, Wolf ML, Chabner BA . Pseudomonas blepharoconjunctivitis: a complication of combination chemotherapy. Arch Ophthalmol 1974;91:490–1.
Kreger AS . Pathogenesis of Pseudomonas aeruginosa ocular diseases. Rev Infect Dis 1983;5:931–5.
Ayliffe GAJ, Barry DR, Lowbury EJL, Roper-Hall MJ, Walker WM . Postoperative infection with Pseudomonas aeruginosa in an eye hospital. Lancet 1966;1:1113–7.
Stanley MM . Bacillus pyocyaneus infections: a review, report of cases and discussion of newer therapy including streptomycin. Am J Med 1947;2:347–67.
Fox SA . Ophthalmic plastic surgery, 5th ed. New York: Grune & Stratton, 1976:64.
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Baldeschi, L., Lupetti, A., Nardi, M. et al. A comparison of skin storage methods for oculoplastic surgery. Eye 12, 714–716 (1998). https://doi.org/10.1038/eye.1998.174
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DOI: https://doi.org/10.1038/eye.1998.174
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