Expression of leptin and adiponectin in BAFF-treated 3T3-L1 adipocytes. At day 8 after inducing differentiation, adipocytes were cultured in a 35-mm dish with 10 ng ml−1 BAFF for 2, 4, 8 or 12 h. As control (CON), cells were cultured in the same condition but without BAFF treatment. Graphs represent mRNA and protein expression of (a) leptin and (b) adiponectin. mRNA analysis was expressed relative to untreated control cells and represent means±s.e. Experiments were conducted twice, each with triplicate samples. Means with letters are significantly different (*P<0.05, **P<0.01 and ***P<0.001) by Student’s t-test.