Abstract
5'-upstream region of the phospholipase C-β2 gene, 810 bp, was cloned and characterized. S1 nuclease mapping and primer extension analyses revealed that a single transcriptional start site locates at 284 nucleotides upstream from the beginning of translation. The 5-upstream region lacks both TATA motif and typical initiator sequence, but retains GC-rich segment. Two putative regulatory regions, a negative region (-636/-588) and a positive region (-98/ -13) were identified in the upstream region of PLC-β2 gene. We suggest that the transcription of PLC-β2 may be regulated by binding of regulatory proteins to the negative and/or positive regulatory regions located in the upstream of the gene.
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This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Yun, E., Lee, SJ., Kim, M. et al. Cloning and characterization of 5'-upstream region of human phospholipase C-β2 gene. Exp Mol Med 33, 76–82 (2001). https://doi.org/10.1038/emm.2001.14
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DOI: https://doi.org/10.1038/emm.2001.14