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Protein, malnutrition and wasting disorders

The effects of proteins and medium-chain fatty acids from milk on body composition, insulin sensitivity and blood pressure in abdominally obese adults

Subjects

Abstract

Background/Objectives:

To investigate whether intake of whey protein and butter naturally enriched in medium-chain fatty acids (MC-SFAs) (C6–C12) affected body composition, insulin sensitivity, blood pressure (BP) and plasma cholesterol concentrations.

Subjects/Methods:

A 12-week randomised, double-blinded, intervention study was completed in 52 abdominally obese adults. Subjects were assigned to one of four dietary supplementations: 63 g per day of milk fat with either high- (8.5 g per day) or low-MC-SFA (6.9 g per day) content combined with 60 g per day of whey or casein.

We examined changes in the body composition by dual-energy X-ray absorption scan, insulin sensitivity using homoeostatic model assessment of insulin resistance (HOMA-IR) and Matsuda index, and diurnal BP and plasma cholesterol concentrations. Two-factor analysis of variance was used to examine the impact of MC-SFA content and protein type.

Results:

We observed that lean body mass increased by 981 g (95% confidence interval (CI): 248–1713; P=0.010) after high-MC-SFA compared with low-MC-SFA supplementation. Concomitantly, total body-fat percentage increased by 0.70 percentage points (95% CI: 0.10–1.31; P=0.024) after intake of low-MC-SFA butter compared with intake of high-MC-SFA butter. Both changes were independent of protein type (P=0.96 and P=0.99, respectively). We found no difference in HOMA-IR, Matsuda index, diurnal BP or plasma cholesterol concentrations related to MC-SFA content or protein type.

Conclusions:

Enhanced intake of MC-SFA increased the lean body mass and caused a significantly lower total body-fat percentage compared with lower intake of MC-SFA. Consequently, the composition of dairy fat should be considered when evaluating the impact of dairy products on body composition.

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References

  1. 1

    Benatar JR, Sidhu K, Stewart RA . Effects of high and low fat dairy food on cardio-metabolic risk factors: a meta-analysis of randomized studies. PLoS One 2013; 8: e76480.

    CAS  Article  Google Scholar 

  2. 2

    Dugan CE, Fernandez ML . Effects of dairy on metabolic syndrome parameters: a review. Yale J Biol Med 2014; 87: 135–147.

    CAS  PubMed  PubMed Central  Google Scholar 

  3. 3

    Soedamah-Muthu SS, Ding EL, Al-Delaimy WK, Hu FB, Engberink MF, Willett WC et al. Milk and dairy consumption and incidence of cardiovascular diseases and all-cause mortality: dose-response meta-analysis of prospective cohort studies. Am J Clin Nutr 2011; 93: 158–171.

    CAS  Article  Google Scholar 

  4. 4

    Gao D, Ning N, Wang C, Wang Y, Li Q, Meng Z et al. Dairy products consumption and risk of type 2 diabetes: systematic review and dose-response meta-analysis. PLoS One 2013; 8: e73965.

    CAS  Article  Google Scholar 

  5. 5

    Abargouei AS, Janghorbani M, Salehi-Marzijarani M, Esmaillzadeh A . Effect of dairy consumption on weight and body composition in adults: a systematic review and meta-analysis of randomized controlled clinical trials. Int J Obes 2012; 36: 1485–1493.

    CAS  Article  Google Scholar 

  6. 6

    Chen M, Pan A, Malik VS, Hu FB . Effects of dairy intake on body weight and fat: a meta-analysis of randomized controlled trials. Am J Clin Nutr 2012; 96: 735–747.

    CAS  Article  Google Scholar 

  7. 7

    Dougkas A, Reynolds CK, Givens ID, Elwood PC, Minihane AM . Associations between dairy consumption and body weight: a review of the evidence and underlying mechanisms. Nutr Res Rev 2011; 24: 72–95.

    Article  Google Scholar 

  8. 8

    Turner KM, Keogh JB, Clifton PM . Dairy consumption and insulin sensitivity: a systematic review of short- and long-term intervention studies. Nutr Metab Cardiovasc Dis 2015; 25: 3–8.

    CAS  Article  Google Scholar 

  9. 9

    Drehmer M, Pereira MA, Schmidt MI, Del Carmen B Molina M, Alvim S, Lotufo PA et al. Associations of dairy intake with glycemia and insulinemia, independent of obesity, in Brazilian adults: the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil). Am J Clin Nutr 2015; 101: 775–782.

    CAS  Article  Google Scholar 

  10. 10

    Pereira MA, Jacobs Jr DR, Van Horn L, Slattery ML, Kartashov AI, Ludwig DS . Dairy consumption, obesity, and the insulin resistance syndrome in young adults: the CARDIA Study. JAMA 2002; 287: 2081–2089.

    Article  Google Scholar 

  11. 11

    Hirahatake KM, Slavin JL, Maki KC, Adams SH . Associations between dairy foods, diabetes, and metabolic health: potential mechanisms and future directions. Metabolism 2014; 63: 618–627.

    CAS  Article  Google Scholar 

  12. 12

    Lamarche B . Review of the effect of dairy products on non-lipid risk factors for cardiovascular disease. J Am Coll Nutr 2008; 27: 741S–746S.

    Article  Google Scholar 

  13. 13

    Park KM, Cifelli CJ . Dairy and blood pressure: a fresh look at the evidence. Nutr Rev 2013; 71: 149–157.

    Article  Google Scholar 

  14. 14

    Ralston RA, Lee JH, Truby H, Palermo CE, Walker KZ . A systematic review and meta-analysis of elevated blood pressure and consumption of dairy foods. J Hum Hypertens 2012; 26: 3–13.

    CAS  Article  Google Scholar 

  15. 15

    Siervo M, Lara J, Chowdhury S, Ashor A, Oggioni C, Mathers JC . Effects of the Dietary Approach to Stop Hypertension (DASH) diet on cardiovascular risk factors: a systematic review and meta-analysis. Br J Nutr 2015; 113: 1–15.

    CAS  Article  Google Scholar 

  16. 16

    Maeno M, Yamamoto N, Takano T . Identification of an antihypertensive peptide from casein hydrolysate produced by a proteinase from lactobacillus helveticus CP790. J Dairy Sci 1996; 79: 1316–1321.

    CAS  Article  Google Scholar 

  17. 17

    Abubakar A, Saito T, Kitazawa H, Kawai Y, Itoh T . Structural analysis of new antihypertensive peptides derived from cheese whey protein by proteinase K digestion. J Dairy Sci 1998; 81: 3131–3138.

    CAS  Article  Google Scholar 

  18. 18

    Ballard KD, Kupchak BR, Volk BM, Mah E, Shkreta A, Liptak C et al. Acute effects of ingestion of a novel whey-derived extract on vascular endothelial function in overweight, middle-aged men and women. Br J Nutr 2013; 109: 882–893.

    CAS  Article  Google Scholar 

  19. 19

    Ballard KD, Bruno RS, Seip RL, Quann EE, Volk BM, Freidenreich DJ et al. Acute ingestion of a novel whey-derived peptide improves vascular endothelial responses in healthy individuals: a randomized, placebo controlled trial. Nutr J 2009; 8: 34–2891-8-34.

    Article  Google Scholar 

  20. 20

    Townsend RR, McFadden CB, Ford V, Cadee JA . A randomized, double-blind, placebo-controlled trial of casein protein hydrolysate (C12 peptide) in human essential hypertension. Am J Hypertens 2004; 17: 1056–1058.

    CAS  Article  Google Scholar 

  21. 21

    Pal S, Ellis V, Dhaliwal S . Effects of whey protein isolate on body composition, lipids, insulin and glucose in overweight and obese individuals. Br J Nutr 2010; 104: 716–723.

    CAS  Article  Google Scholar 

  22. 22

    Nordic Council of Ministers. Nordic Nutrition Recommendations 2012. Integrating Nutrition And Physical Activity, 5th edn. Copenhagen: Copenhagen, Denmark, 2014..

  23. 23

    Johnson RC, Young SK, Cotter R, Lin L, Rowe WB . Medium-chain-triglyceride lipid emulsion: metabolism and tissue distribution. Am J Clin Nutr 1990; 52: 502–508.

    CAS  Article  Google Scholar 

  24. 24

    Papamandjaris AA, MacDougall DE, Jones PJ . Medium chain fatty acid metabolism and energy expenditure: obesity treatment implications. Life Sci 1998; 62: 1203–1215.

    CAS  Article  Google Scholar 

  25. 25

    Labarthe F, Gelinas R, Des Rosiers C . Medium-chain fatty acids as metabolic therapy in cardiac disease. Cardiovasc Drugs Ther 2008; 22: 97–106.

    CAS  Article  Google Scholar 

  26. 26

    Marten B, Pfeuffer M, Schrezenmeir J . Medium-chain triglycerides. Int Dairy J 2006; 16: 1374–1382.

    CAS  Article  Google Scholar 

  27. 27

    Ericson U, Hellstrand S, Brunkwall L, Schulz CA, Sonestedt E, Wallstrom P et al. Food sources of fat may clarify the inconsistent role of dietary fat intake for incidence of type 2 diabetes. Am J Clin Nutr 2015; 101: 1065–1080.

    CAS  Article  Google Scholar 

  28. 28

    Mumme K, Stonehouse W . Effects of medium-chain triglycerides on weight loss and body composition: a meta-analysis of randomized controlled trials. J Acad Nutr Diet 2015; 115: 249–263.

    Article  Google Scholar 

  29. 29

    Bueno NB, de Melo IV, Florêncio TT, Sawaya AL . Dietary medium-chain triacylglycerols versus long-chain triacylglycerols for body composition in adults: systematic review and meta-analysis of randomized controlled trials. J Am Coll Nutr 2015; 34: 175–183.

    CAS  Article  Google Scholar 

  30. 30

    Bohl M, Bjornshave A, Rasmussen KV, Schioldan AG, Amer B, Larsen MK et al. Dairy proteins, dairy lipids, and postprandial lipemia in persons with abdominal obesity (DairyHealth): a 12-wk, randomized, parallel-controlled, double-blinded, diet intervention study. Am J Clin Nutr 2015; 101: 870–878.

    CAS  Article  Google Scholar 

  31. 31

    Larsen MK, Hymoller L, Brask-Pedersen DB, Weisbjerg MR . Milk fatty acid composition and production performance of Danish Holstein and Danish Jersey cows fed different amounts of linseed and rapeseed. J Dairy Sci 2012; 95: 3569–3578.

    CAS  Article  Google Scholar 

  32. 32

    Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC . Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia 1985; 28: 412–419.

    CAS  Article  Google Scholar 

  33. 33

    Matsuda M, DeFronzo RA . Insulin sensitivity indices obtained from oral glucose tolerance testing: comparison with the euglycemic insulin clamp. Diabetes Care 1999; 22: 1462–1470.

    CAS  Article  Google Scholar 

  34. 34

    O'Brien E, Waeber B, Parati G, Staessen J, Myers MG . Blood pressure measuring devices: recommendations of the European Society of Hypertension. BMJ 2001; 322: 531–536.

    CAS  Article  Google Scholar 

  35. 35

    Lavie CJ, De Schutter A, Patel DA, Romero-Corral A, Artham SM, Milani RV . Body composition and survival in stable coronary heart disease: impact of lean mass index and body fat in the ‘obesity paradox’. J Am Coll Cardiol 2012; 60: 1374–1380.

    Article  Google Scholar 

  36. 36

    Ortega FB, Lavie CJ, Blair SN . Obesity and cardiovascular disease. Circ Res 2016; 118: 1752–1770.

    CAS  Article  Google Scholar 

  37. 37

    Jensen MD . Role of body fat distribution and the metabolic complications of obesity. J Clin Endocrinol Metab 2008; 93: S57–S63.

    CAS  Article  Google Scholar 

  38. 38

    St-Onge MP, Ross R, Parsons WD, Jones PJ . Medium-chain triglycerides increase energy expenditure and decrease adiposity in overweight men. Obes Res 2003; 11: 395–402.

    CAS  Article  Google Scholar 

  39. 39

    Dulloo AG, Fathi M, Mensi N, Girardier L . Twenty-four-hour energy expenditure and urinary catecholamines of humans consuming low-to-moderate amounts of medium-chain triglycerides: a dose-response study in a human respiratory chamber. Eur J Clin Nutr 1996; 50: 152–158.

    CAS  PubMed  Google Scholar 

  40. 40

    Scalfi L, Coltorti A, Contaldo F . Postprandial thermogenesis in lean and obese subjects after meals supplemented with medium-chain and long-chain triglycerides. Am J Clin Nutr 1991; 53: 1130–1133.

    CAS  Article  Google Scholar 

  41. 41

    Rego Costa AC, Rosado EL, Soares-Mota M . Influence of the dietary intake of medium chain triglycerides on body composition, energy expenditure and satiety: a systematic review. Nutr Hosp 2012; 27: 103–108.

    CAS  PubMed  Google Scholar 

  42. 42

    Weisbjerg MR, Wiking L, Kristensen NB, Lund P . Effects of supplemental dietary fatty acids on milk yield and fatty acid composition in high and medium yielding cows. J Dairy Res 2008; 75: 142–152.

    CAS  Article  Google Scholar 

  43. 43

    Vessby B, Uusitupa M, Hermansen K, Riccardi G, Rivellese AA, Tapsell LC et al. Substituting dietary saturated for monounsaturated fat impairs insulin sensitivity in healthy men and women: The KANWU Study. Diabetologia 2001; 44: 312–319.

    CAS  Article  Google Scholar 

  44. 44

    Rasmussen BM, Vessby B, Uusitupa M, Berglund L, Pedersen E, Riccardi G et al. Effects of dietary saturated, monounsaturated, and n-3 fatty acids on blood pressure in healthy subjects. Am J Clin Nutr 2006; 83: 221–226.

    CAS  Article  Google Scholar 

  45. 45

    Rietman A, Schwarz J, Blokker BA, Siebelink E, Kok FJ, Afman LA et al. Increasing protein intake modulates lipid metabolism in healthy young men and women consuming a high-fat hypercaloric diet. J Nutr 2014; 144: 1174–1180.

    CAS  Article  Google Scholar 

  46. 46

    Engel S, Tholstrup T . Butter increased total and LDL cholesterol compared with olive oil but resulted in higher HDL cholesterol compared with a habitual diet. Am J Clin Nutr 2015; 102: 309–315.

    CAS  Article  Google Scholar 

  47. 47

    Hermansen K . Diet, blood pressure and hypertension. Br J Nutr 2000; 83(Suppl 1):S113–S119.

    CAS  Article  Google Scholar 

  48. 48

    Pal S, Ellis V . The chronic effects of whey proteins on blood pressure, vascular function, and inflammatory markers in overweight individuals. Obesity (Silver Spring) 2010; 18: 1354–1359.

    CAS  Article  Google Scholar 

  49. 49

    Abdullah MM, Cyr A, Lepine MC, Labonte ME, Couture P, Jones PJ et al. Recommended dairy product intake modulates circulating fatty acid profile in healthy adults: a multi-centre cross-over study. Br J Nutr 2015; 113: 435–444.

    CAS  Article  Google Scholar 

  50. 50

    Poppitt SD, Kilmartin P, Butler P, Keogh GF . Assessment of erythrocyte phospholipid fatty acid composition as a biomarker for dietary MUFA, PUFA or saturated fatty acid intake in a controlled cross-over intervention trial. Lipids Health Dis 2005; 4: 30.

    Article  Google Scholar 

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Acknowledgements

We thank the study participants for their contribution and time. We thank Eva Mølgaard Jensen, Lene Trudsø and Tove Skrumsager for their excellent technical assistance. We thank Allan Stubbe Christensen, Anne Grethe Schioldan, Annemarie Kruse, Kia Valum Rasmussen, Peter Reiter and Zohrah Rahmatyar for their assistance in handling the study participants. We thank librarian Edith Clausen and biostatistician Mogens Erlandsen for their valuable assistance. Grants were from the Danish Council for Strategic Research (DSF 0603-004193), the Danish Dairy Research Foundation and the Arla Food Ingredients Group P/S. Protein powder was provided by Arla Foods Ingredients Group P/S. AB was supported by research grants from The Danish Diabetes Academy supported by the Novo Nordisk Foundation and Aarhus University. Arla Foods Ingredients Group P/S contributed to the study design, but had no role in the conduct of the study, the analysis of data or in the writing of this article.

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Correspondence to M Bohl.

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Bohl, M., Bjørnshave, A., Larsen, M. et al. The effects of proteins and medium-chain fatty acids from milk on body composition, insulin sensitivity and blood pressure in abdominally obese adults. Eur J Clin Nutr 71, 76–82 (2017). https://doi.org/10.1038/ejcn.2016.207

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