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Interventions and public health nutrition

Effects of whey proteins and carbohydrates on the efficacy of resistance training in elderly people: double blind, randomised controlled trial

Abstract

Background/objectives:

A few previous studies indicate that protein supplementation increases gains in muscle mass and strength during a resistance exercise program. The purpose of this study was to investigate whether whey protein supplementation results in greater increases in lean body mass, muscle strength and physical function in elderly individuals during 12 weeks of resistance exercise when compared to isocaloric carbohydrate supplementation.

Subjects/Methods:

A total of 161 men and women, 65–91 years old, participated in a randomized, controlled, double-blind intervention study, involving dietary supplementation and a 12-week resistance exercise program, designed to increase muscle mass and strength of all major muscle groups. Participants exercised three times a week and received either 20 g of whey protein (n=83) or isocaloric carbohydrate (n=78) in liquid form immediately after each workout. Data were obtained at baseline and end point.

Results:

The primary outcomes, lean body mass, strength and physical function increased significantly during the course of the study. Type of dietary supplementation did not influence gains in lean body mass (P=0.365), quadriceps strength (P=0.776) or performance during a 6-min walk (P=0.726) or a timed up-and-go test (P=0.151). Twenty participants discontinued the intervention.

Conclusions:

Ingestion of 20 g of whey protein immediately after resistance exercise three times per week, does not lead to greater gains in lean body mass, strength and physical function in elderly people with sufficient energy and protein intakes when compared to isocaloric carbohydrate.

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References

  1. Wolfson L, Judge J, Whipple R, King M . Strength is a major factor in balance, gait, and the occurrence of falls. J Gereontol A Biol Sci Med Sci 1995; 50: 64–67.

    Google Scholar 

  2. Wang C, Bai L . Sarcopenia in the elderly: Basic and clinical issues. Geriatr Gerontol Int 2012; 12: 388–396.

    Article  Google Scholar 

  3. Kan GAV, André E, Bischoff-Ferrari HA, Boirie Y, Onder G, Pahor M et al. Carla task force on sarcopenia: Propositions for clinical trials (review). J Nutr Health Aging 2009; 13: 700–707.

    Article  Google Scholar 

  4. Morley JE, Argiles JM, Evans WJ, Bhasin S, Cella D, Deutz NE et al. Nutritional recommendations for the management of sarcopenia. J Am Med Dir Assoc 2010; 11: 391–396.

    Article  Google Scholar 

  5. Cermak NM, Res PT, de Groot LCPGM, Saris WHM, van Loon LJC . Protein supplementation augments the adaptive response of skeletal muscle to resistance-type exercise training: a meta-analysis. Am J Clin Nutr 2012; 96: 1454–1464.

    Article  CAS  Google Scholar 

  6. Kim J-S, Wilson J-M, Lee S-R . Dietary implications on mechanisms of sarcopenia: roles of protein, amino acids and antioxidants (review). J Nutr Biochem 2010; 21: 1–13.

    Article  Google Scholar 

  7. Hulmi JJ, Lockwood CM, Stout JR . Effect of protein/essential amino acids and resistance training on skeletal muscle hypertrophy: a case for whey protein (review). Nutr Metab 2010; 177: 51.

    Article  Google Scholar 

  8. Cribb PJ, Williams AD, Hayes A, Carey MF . The effect of whey isolate on strength, body composition and plasma glutamine. Int J Sports Nutr 2006; 16: 494–509.

    CAS  Google Scholar 

  9. Tang JE, Moore DR, Kujbida GW, Tarnopolsky MA, Phillips SM . Ingestion of whey hydrolysate, casein, or soy protein isolate: effects on mixed muscle protein synthesis at rest and following resistance exercise in young men. J Appl Physiol 2009; 107: 987–992.

    Article  CAS  Google Scholar 

  10. Pennings B, Boirie Y, Senden JMG, Gijsen AP, Kuipers H, van Loon LJ . Whey protein stimulates postprandial muscle protein accretion more effectively than do casein and casein hydrolysate in older men. Am J Clin Nutr 2011; 93: 997–1005.

    Article  CAS  Google Scholar 

  11. Hayes A, Cribb PJ . Effect of whey protein isolate on strength, body composition and muscle hypertrophy during resistance training. Curr Opin Clin Nutr 2008; 11: 40–44.

    Article  CAS  Google Scholar 

  12. Volpi E, Mittendorfer B, Wolf SE, Wolfe RR . Oral amino acids stimulate muscle protein anabolism in the elderly despite higher first pass splanchnic extraction. Am J Physiol 1999; 277: E513–E520.

    CAS  PubMed  Google Scholar 

  13. Smith K, Barua JM, Watt PW, Scrimgeour CM, Rennie MJ . Flooding with 1-13Cleucine stimulates human muscle protein incorporation of continuously infused L-1-13Cvaline. Am J Physiol 1992; 262: E372–E376.

    CAS  PubMed  Google Scholar 

  14. Volpi E, Kobayashi H, Sheffield-Moore M, Mittendorfer B, Wolfe RR . Essential amino acids are primarily responsible for the amino acid stimulation of muscle protein anabolism in healthy elderly adults. Am J Clin Nutr 2003; 78: 250–258.

    Article  CAS  Google Scholar 

  15. Smith K, Reynolds N, Downie S, Patel A, Rennie MJ . Effects of flooding amino acids on incorporation of labelled amino acids into human muscle protein. Am J Physiol 1998; 275: E73–E78.

    CAS  PubMed  Google Scholar 

  16. Podsiadlo D, Richardson S . The timed up & go: a test of basic functional mobility for frail elderly persons. J Am Geriatr Soc 1991; 39: 142–148.

    Article  CAS  Google Scholar 

  17. American Thoracic Society Statement. Guidelines for the six-minute walk test. Am J Respir Crit Care Med 2002; 166: 111–117.

    Article  Google Scholar 

  18. Physical activity. In: Alexander J, Anderssen SA, Aro A, Becker W, Fogelholm M, Lyhne N et al (eds). Nordic Nutrition Recommendations 2004 4th edn Nordic Council of Ministers: Copenhagen, Denmark, 2004, pp 139–156.

    Google Scholar 

  19. Protein. In: Alexander J, Anderssen SA, Aro A, Becker W, Fogelholm M, Lyhne N et al (eds) Nordic Nutrition Recommendations 2004 4th edn Nordic Council of Ministers: Copenhagen, Denmark, 2004, pp 199–211.

    Google Scholar 

  20. Candow DG, Chilibeck PD, Facci M, Abeysekara S, Zello GA . Protein supplementation before and after resistance training in older men. Eur J Appl Physiol 2006; 97: 548–556.

    Article  CAS  Google Scholar 

  21. Maesta N, Nahas EAP, Nahas-Neto J, Orsatti FL, Fernandes CE, Traiman P et al. Effects of soy protein and resistance exercise on body composition and blood lipids in postmenopausal women. Maturitas 2007; 56: 350–358.

    Article  CAS  Google Scholar 

  22. Verdijk LB, Jonkers R, Gleeson B, Beelen M, Meijer K, Savelberg HH et al. Protein supplementation before and after exercise does not further augment skeletal muscle hypertrophy after resistance training in elderly men. Am J Clin Nutr 2009; 89: 608–616.

    Article  CAS  Google Scholar 

  23. Kukuljan S, Nowson CA, Sanders K, Daly RM . Effects of resistance exercise and fortified milk on skeletal muscle mass, muscle size, and functional performance in middle-aged and older men: an 8-mo randomized controlled trial. J Appl Physiol 2009; 107: 1864–1873.

    Article  CAS  Google Scholar 

  24. Godard MP, Williamson DL, Trappe SW . Oral amino-acid provision does not affect muscle strength or size gains in older men. Med Sci Sports Exerc 2002; 34: 1126–1131.

    Article  CAS  Google Scholar 

  25. Holm L, Olesen JL, Matsumoto K, Doi T, Mizuno M, Alsted TJ et al. Protein containing nutrient supplementation following strength training enhances the effect on muscle mass, strength, and bone formation in postmenopausal women. J Appl Physiol 2008; 105: 274–281.

    Article  CAS  Google Scholar 

  26. Bonnefoy M, Cornu C, Normand S, Boutitie F, Bugnard F, Rahmani A et al. The effects of exercise and protein-energy supplements on body composition and muscle function in frail elderly individuals: a long-term controlled randomised study. Br J Nutr 2003; 89: 731–738.

    Article  CAS  Google Scholar 

  27. Tieland M, van de Rest O, Dirks ML, van der Zwaluw N, Mensink M, van Loon LJ et al. Protein supplementation improves physical performance in frail elderly people: a randomized double-blind, placebo-controlled trial. J Am Med Dir Assoc 2012; 13: 720–726.

    Article  Google Scholar 

  28. Cribb PJ, Williams AD, Stathis CG, Carey MF, Hayes A . Effects of whey isolate, creatine, and resistance training on muscle hypertrophy. Med Sci Sports Exerc 2007; 39: 298–307.

    Article  CAS  Google Scholar 

  29. Hulmi JJ, Kovanen V, Salänne H, Kraemer VJ, Häkkinen K, Mero AA et al. Acute and long-term effects of resistance exercise with or without protein ingestion on muscle hypertrophy and gene expression. Amino Acids 2009; 37: 297–308.

    Article  CAS  Google Scholar 

  30. Willoughby DS, Stout JR, Wilborn CD . Effects of resistance training and protein plus amino acid supplementation on muscle anabolism, mass, and strength. Amino Acids 2007; 32: 467–477.

    Article  CAS  Google Scholar 

  31. Chromiak JA, Smedley B, Carpenter B, Brown R, Koh YS, Lamberth JG et al. Effect of a 10-week strength training program and recovery drink on body composition, muscular strength and endurance, and anaerobic power and capacity. Nutrition 2004; 20: 420–427.

    Article  Google Scholar 

  32. DeNysschen CA, Burton HW, Horvath PJ, Leddy JJ, Browne R . Resistance training with soy vs whey protein supplements in hyperlipidemic males. J Int Soc Sports Nutr 2009; 6: 1–9.

    Article  Google Scholar 

  33. Paddon-Jones D, Sheffield-Moore M, Zhang XJ, Volpi E, Wolf SE, Aarsland A et al. Amino acid ingestion improves muscle protein synthesis in the young and elderly. Am J Physiol Endocrinol Metab 2004; 286: E321–E328.

    Article  CAS  Google Scholar 

  34. Katsanos CS, Kobayashi H, Sheffield-Moore M, Aarsland A, Wolfe RR . Aging is associated with diminished accretion of muscle proteins after the ingestion of a small bolus of essential amino acids. Am J Clin Nutr 2005; 82: 1065–1073.

    Article  CAS  Google Scholar 

  35. Katsanos CS, Kobayashi H, Sheffield-Moore M, Aarsland A, Wolfe RR . A high proportion of leucine is required for optimal stimulation of the rate of muscle protein synthesis by essential amino acids in the elderly. Am J Physiol Endocrinol Metab 2006; 91: E281–E387.

    Google Scholar 

  36. Paddon-Jones D, Rasmussen BB . Dietary protein recommendations and the prevention of sarcopenia. Curr Opin Clin Nutr 2009; 12: 86–90.

    Article  CAS  Google Scholar 

  37. Walzem RL, Dillard CJ, German JB . Whey components: millenia of evolution create functionalities for mammalian nutrition: what we know and what we may be overlooking (review). Crit Rev Food Sci Nutr 2002; 42: 353–375.

    Article  CAS  Google Scholar 

  38. Pennings B, Groen B, de Lange A, Gijsen AP, Zorenc AH, Senden JM et al. Amino acid absorption and subsequent muscle protein accretion following graded intakes of whey protein in elderly men. Am J Physiol Endocrinol Metab 2012; 302: E992–E999.

    Article  CAS  Google Scholar 

  39. Volpi E, Mittendorfer B, Rasmussen BB, Wolfe RR . The response of muscle protein anabolism to combined hyperaminoacidemia and glucose-induced hyperinsulinemia is impaired in the elderly. J Clin Endocrinol Metab 2000; 85: 4481–4490.

    CAS  PubMed  PubMed Central  Google Scholar 

  40. Staples AW, Burd NA, West DWD, Currie KD, Atherton PJ, Moore DR et al. Carbohydrate does not augment exercise-induced protein accretion versus protein alone. Med Sci Sport Exer 2010; 43: 1154–1161.

    Article  Google Scholar 

  41. Bosse JD, Dixon BM . Dietary protein to maximize resistance training: a review and examination of protein spread and change theories. J Int Soc Sports Nutr 2012; 9: 42.

    Article  CAS  Google Scholar 

  42. Candow DG, Chilibeck PD . Timing of creatine or protein supplementation and resistance training in the elderly. Appl Physiol Nutr Metab 2008; 33: 184–190.

    Article  CAS  Google Scholar 

  43. Thalacker-Mercer AE, Petrella JK, Bamman MM . Does habitual dietary intake influence myofiber hypertrophy in response to resistance training? A cluster analysis. Appl Physiol Nutr Metab 2009; 34: 632–639.

    Article  Google Scholar 

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Acknowledgements

The study was funded by the Icelandic Technology Development Fund (No. 071323008), Research Fund of the University of Iceland, Landspitali University Hospital Research Fund and the Helga Jonsdottir and Sigurlidi Kristjansson Geriatric Research Fund. The trial is registered at the US National Library of Medicine (Nr. NCT01074879).

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Correspondence to A Arnarson.

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Arnarson, A., Gudny Geirsdottir, O., Ramel, A. et al. Effects of whey proteins and carbohydrates on the efficacy of resistance training in elderly people: double blind, randomised controlled trial. Eur J Clin Nutr 67, 821–826 (2013). https://doi.org/10.1038/ejcn.2013.40

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  • DOI: https://doi.org/10.1038/ejcn.2013.40

Keywords

  • elderly
  • protein
  • whey protein
  • resistance exercise
  • muscle strength
  • muscle mass

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