Cocoa products, which are rich sources of flavonoids, have been shown to reduce blood pressure and the risk of cardiovascular disease. Dark chocolate contains saturated fat and is a source of dietary calories; consequently, it is important to determine whether consumption of dark chocolate adversely affects the blood lipid profile. The objective was to examine the effects of dark chocolate/cocoa product consumption on the lipid profile using published trials. A detailed literature search was conducted via MEDLINE (from 1966 to May 2010), CENTRAL and ClinicalTrials.gov for randomized controlled clinical trials assessing the effects of flavanol-rich cocoa products or dark chocolate on lipid profile. The primary effect measure was the difference in means of the final measurements between the intervention and control groups. In all, 10 clinical trials consisting of 320 participants were included in the analysis. Treatment duration ranged from 2 to 12 weeks. Intervention with dark chocolate/cocoa products significantly reduced serum low-density lipoprotein (LDL) and total cholesterol (TC) levels (differences in means (95% CI) were −5.90 mg/dl (−10.47, −1.32 mg/dl) and −6.23 mg/dl (−11.60, −0.85 mg/dl), respectively). No statistically significant effects were observed for high-density lipoprotein (HDL) (difference in means (95% CI): −0.76 mg/dl (−3.02 to 1.51 mg/dl)) and triglyceride (TG) (−5.06 mg/dl (−13.45 to 3.32 mg/dl)). These data are consistent with beneficial effects of dark chocolate/cocoa products on total and LDL cholesterol and no major effects on HDL and TG in short-term intervention trials.
Dietary choices are strongly influenced by the taste and texture of foods. Fats are to a large extent responsible for the sensory properties of many foods and, thereby, greatly contribute to eating pleasure. Consumption of foods rich in saturated fatty acids and cholesterol, however, has long been recognized as an important precursor for the development of coronary heart disease. Intake of saturated fatty acids and cholesterol is associated with elevated cholesterol and low-density lipoprotein (LDL) concentrations in the blood (NCEP, 1993; Judd et al., 1994; Temme et al., 1996; Hunter et al., 2010).
Chocolate and cocoa are produced from cacao beans, the seed of Theobroma cacao, and are known to contain fats (the dry weight of whole cacao beans is composed of 50–57% lipid, often called cocoa butter (Hannum and Erdman, 2000)). This cocoa butter, predominantly found in dark chocolate, is composed on average of 33% oleic acid, 25% palmitic acid, and 33% of stearic acid. (http://www.nal.usda.gov/fnic/foodcomp/search/), the latter two being saturated fats. Cocoa products are also very rich in plant phytochemicals, especially flavonoids, which are now objects of increased scientific attention due to their potential health benefits (Engler et al., 2004; Grassi et al., 2005a; Wang-Polagruto et al., 2006; Almoosawi et al., 2010).
Previous studies have suggested that dark chocolate consumption reduces blood pressure (Grassi et al., 2005b; Grassi et al., 2008), improves insulin sensitivity as shown by significantly higher QUICKI (quantitative insulin sensitivity check index) measurements (Grassi et al., 2008), improves vascular endothelial function and reverses vascular dysfunction (Engler et al., 2004; Grassi et al., 2005b; Wang-Polagruto et al., 2006), reduces insulin resistance as evidenced by significantly lower HOMA-IR (homeostasis model assessment of insulin resistance) (Grassi et al., 2005a) measurements, and increases serum total antioxidant capacity (Wan et al., 2001).
Despite solid evidence on the beneficial effects of dark chocolate on blood pressure, limited data exist on the effects of dark chocolate on blood lipids. One clinical trial indicated that regular ingestion of dark chocolate may have no adverse effects on serum lipid profile (Crews et al., 2008), whereas others have suggested that intake of dark chocolate reduced serum LDL cholesterol and triglyceride (TG) levels (Engler et al., 2004; Grassi et al., 2005b), and increased serum high-density lipoprotein (HDL) cholesterol measurements (Mursu et al., 2004). An earlier meta-analysis of eight randomized trials involving 215 subjects reported that an intervention with dark chocolate was associated with a significant reduction in serum LDL in subjects with cardiovascular disease risk factors (Jia et al., 2010) compared with placebo. However, that meta-analysis did not assess the effect of dark chocolate or cocoa on serum TG concentrations and it did not include some important recent studies.
Dark chocolate is a food consumed frequently and widely all over the world. It is therefore relevant to understand its net benefits on health in order to help the public make informed choices. Hence, we sought to review current evidence on the effects of dark chocolate/cocoa products consumption on serum LDL, HDL and TGs using completed randomized trials.
Materials and methods
Search strategy and study selection
We searched MEDLINE (1966 to May 2010), CENTRAL (The Cochrane Controlled Clinical Trials Register) and the ClinicalTrials.gov website to identify randomized controlled trials examining the effect of dark chocolate or cocoa-containing beverages on blood lipid profile. Our search was restricted to papers published in the English language. For MEDLINE search, we used the MeSH (major subject heading) term ‘cacao’ or the text words ‘cacao’, ‘flavonoids’ or ‘chocolate’; ‘cholesterol, LDL’; ‘cholesterol, HDL’; and ‘TGs, cholesterol’. The search was limited to the criteria ‘clinical trials’, ‘English’ and ‘human’. CENTRAL and the ClinicalTrials.gov website were searched using ‘chocolate, cocoa or cacao’ as free terms without further restrictions applied to the search. Inspection of the reference list of all identified articles was also conducted. The latter method was repeated until all potentially relevant articles from these sources were identified. Retrieved studies were included if they met the following criteria: (a) were investigating flavanol-rich cocoa products; (b) had a randomized controlled parallel-arm or crossover design; (c) included at least 14 subjects, 18 years and older; and (d) reported ‘baseline’ and ‘end of intervention’ mean and standard deviation values of lipid measurements for the active (intervention) and control groups.
Unpublished materials and conference abstracts were excluded from the review as they lacked the necessary detail needed for data synthesis. Single-dose trials were also not included in the analysis because we wanted to report the effects of habitual intake of flavanol-containing cocoa products. Care was taken not to include data from multiple publications of the same population. Furthermore, we excluded studies in which cocoa intake was mixed with other dietary treatments.
Data extraction and quality assessment
Data were extracted by the lead author. Extracted data included the study characteristics (first author's name; year of publication; number and age range of participants; study design; polyphenol amounts in the active and control arms; duration of study; health characteristics of the study population; and location of the study). We also extracted information on the baseline and final concentrations (or net changes) of serum total cholesterol (TC), LDL cholesterol, HDL cholesterol, and TGs. Lead and senior authors all agreed on the eligibility criteria of included studies.
Quality of the studies was evaluated using the validated Jadad score instrument (Jadad et al., 1996) with criteria that include: randomization, adequacy of sequence generation, double blinding, and description of drop-outs. The maximum possible score was 5.
Data synthesis and statistical analysis
The effect size used in this investigation was the ‘difference in means’ between the two treatment groups. We utilized serum cholesterol concentration obtained at the end of each intervention. Studies that reported results in mmol/l were converted to mg/dl using the standard conversion factors (which was a division of the mmol/l value by 0.02586 for TC, LDL and HDL; and by 0.01129 for TG). Standard errors were also converted to standard deviations where necessary. There was a wide variation in the pattern by which the included studies reported their findings.
One trial presented the means and standard deviation of ‘baseline’ and ‘end of treatment’ values of the two study groups, as well as the change from baseline (with standard deviations) in the two groups (Crews et al., 2008). Another reported the means and standard deviation of the ‘baseline’ and ‘end of treatment’ values of the different intervention groups, as well as the ‘difference of differences’ between the two intervention groups (Muniyappa et al., 2008). Some studies reported only mean values (with standard deviation/errors) of ‘baseline’ and ‘end of treatment’ values of the intervention groups (Wan et al., 2001; Engler et al., 2004; Fraga et al., 2005; Grassi et al., 2005a, 2005b; Balzer et al., 2008; Almoosawi et al., 2010); One study presented only the ‘difference from baseline’ in both intervention groups with their standard deviations (Davison et al., 2008). For studies that reported only the values of baseline and end of treatment values, the difference was calculated by direct subtraction of the means, and the standard deviation was calculated using the following formula:
where R is the correlation coefficient calculated from the study that contained all the required values (see above), according to the Cochrane Handbook (http://www.cochrane-handbook.org/) for Systematic Reviews of Interventions (Julian PT Higgins and Sally Green, 2009). Given that only one study provided the means (and standard deviation) of ‘baseline’ and ‘end of treatment’ values for the two intervention groups as well as the ‘difference of differences’ between the groups, we were reluctant to impute the unreported variance for the remaining nine studies by the correlation coefficient method, as this intrinsically assumes that treatment has no effect on the values of the variance. The effect measure was therefore computed as the difference in the postintervention values of serum TC, HDL, LDL and TGs between the active arm and control groups, following dark chocolate or cocoa intake. Additional analyses were performed according to the Cochrane Handbook for Systematic Reviews of Interventions (Higgins and Green, 2009).
Heterogeneity across studies was assessed by the Cochrane Qtest, and P<0.10 was considered statistically significant for heterogeneity. The magnitude of heterogeneity was evaluated by the I2 statistic (percentage of the variability in effect estimates that is due to heterogeneity rather than sampling error). Whenever the test for heterogeneity was statistically significant, the estimate of the difference was calculated using the random effects model, according to DerSimonian and Laird (1986). Data synthesis and statistical analyses were completed using Cochrane Collaboration Review Manager (RevMan software version 5.0; Cochrane Collaboration, Oxford, UK) (http://www.cc-ims.net/revman) and Microsoft Office Excel 2003 package (Microsoft Corporation, USA).
Most of the studies (1491) produced by our initial search were dropped because their topics were clearly irrelevant to the current project. A total of 20 articles met our inclusion criteria and their full texts were reviewed. Four studies were dropped because flavanol was part of a mixed dietary test regimen (Kris-Etherton et al., 1993; Mustad et al., 1993; Allen et al., 2008) or due to lack of randomization (Baba et al., 2007). Five studies were not included because they did not report sufficient details on blood lipid measurements (Kris-Etherton and Mustad, 1994; Polagruto et al., 2006; Wang-Polagruto et al., 2006; Taubert et al., 2007; Grassi et al., 2008). One study was excluded because of the lack of a control group (Hamed et al., 2008). Another was excluded because it was comparing a milk chocolate intervention with a high carbohydrate diet (Kris-Etherton et al., 1994). A flow chart depicting our selection process is shown in Figure 1. The article (Taubert et al., 2007) was excluded because it did not report (or provide on request) the end-of-intervention values for LDL, HDL and TG measurements.
In all, 10 articles were included in our analyses with a total of 320 individuals. Characteristics of the included studies are presented in Table 1. The duration of the intervention ranged from 2 to 12 weeks. Five of the studies were conducted on healthy subjects; two each were carried out on hypertensive and obese subjects. The last study involved diabetic patients on hypoglycemic agents.
The mean age was 41.7 years (range 21–80 years). Eight of the studies were comparing flavanol-rich cocoa or dark chocolate with either flavanol-poor white chocolate or a matching placebo.
All the included studies were randomized and controlled (parallel arm or crossover). Five were double blind (Engler et al., 2004; Balzer et al., 2008; Crews et al., 2008; Davison et al., 2008; Muniyappa et al., 2008) and only one study (Balzer et al., 2008) described an adequate method of sequence generation. Seven studies reported details of dropouts and withdrawals (Engler et al., 2004; Grassi et al., 2005a; Balzer et al., 2008; Crews et al., 2008; Davison et al., 2008; Muniyappa et al., 2008; Almoosawi et al., 2010). Overall, five of the studies obtained a Jadad score of ⩾3 (high quality) (Table 1).
The postintervention difference in mean LDL concentration (95% CI) comparing active treatment with placebo was −5.90 mg/dl (−10.47, −1.32 mg/dl) (Figure 2). Corresponding differences for HDL, TG and TC were −0.76 mg/dl (−3.03, 1.51 mg/dl) (Figure 3), −5.06 mg/dl (−13.45, 3.32 mg/dl) (Figure 4) and −6.23 mg/dl (−11.60, −0.85 mg/dl), respectively. There was evidence for heterogeneity for LDL (I2=32%) and TC (I2=24%), but not for HDL or TG (I2 of 0% each).
Subgroup analysis was carried out with the data segregated by study duration, study design, flavanol dose, location and health status of participants (Table 2). Trials of short duration (about 2 weeks) showed a significant reduction in serum LDL (−8.44 mg/dl, −14.23 to −2.64 mg/dl), but a nonsignificant reduction in serum HDL and TG levels (Table 2). Studies with longer duration (4–12 weeks) showed a nonsignificant reduction in LDL, HDL and TG levels. The subgroup analyses by study design showed nonsignificant reductions in LDL and HDL serum levels, howbeit, the crossover studies provided narrower interval estimates (than those of the parallel-arm design). Though the formulation of the administered cocoa varied among the studies, the investigators largely reported the flavanol content of their intervention, hence, we also examined a possible dose-effect relationship. Five of the studies administered daily flavanol doses of less than 500 mg; the others administered 500 mg or more. None of the results was statistically significant, but daily flavanol doses of less than 500 mg seemed to produce more marked reduction in LDL concentrations. Subgroup analyses by health status also produced nonsignificant findings, though greater reductions in LDL and TG concentrations were recorded in participants with documented cardiovascular disease risk factors. Analysis by location in which study was carried out revealed a significant reduction in serum LDL concentration −7.13 mg/dl (−14.26, −0.00 mg/dl) for the trials carried out in Europe, others were not significant (Table 2).
Sensitivity analysis that excluded the lower quality studies showed that habitual cocoa intake did not significantly reduce serum lipid concentration (Table 2). When we excluded the two studies (Wan et al., 2001; Fraga et al., 2005) that did not compare flavanol-rich cocoa (or dark chocolate) with flavanol-poor white chocolate (or a matching placebo), we observed a significant reduction in LDL concentration (Table 2).
Our analyses showed a statistically significant reduction in LDL and TC after intervention with dark chocolate/cocoa products. There was also a nonsignificant reduction in serum TG and HDL cholesterol with ingestion of dark chocolate/cocoa products when compared with placebo. The dark chocolate/cocoa effect seemed stronger in subjects with higher risk of cardiovascular disease and in studies with a relatively shorter duration.
This observed reduction may be attributable to flavonoids contained in cocoa and dark chocolate. Flavan-3-ols in cocoa are present as monomers, oligomers or polymers, better known as procyanidins, and generally are thought to inhibit cholesterol absorption as well as the expression of LDL cholesterol receptors (Matsui et al., 2005). The degree to which LDL and TC levels were reduced in this analysis reflects some measure of potency of the cocoa regimen. Our results are consistent with a similar review (Jia et al., 2010) conducted on eight studies, which reported that intervention with dark chocolate/cocoa products led to LDL reduction by 5.87 mg/dl (−11.13, −0.61 mg/dl) and TC reduction by 5.82 mg/dl (95% CI: −12.39, 0.76 mg/dl) compared with placebo. In addition, we are reporting a nonsignificant reduction in mean serum TG levels by 5.06 mg/dl (95% CI: −13.45, 3.32 mg/dl).
The obvious challenge of blinding (when it comes to chocolate products) has been raised as a possible reason why results are not entirely consistent among individual studies. However, when we excluded studies that compared dark chocolate with white chocolate (or in which participants were not properly blinded), our findings were essentially unchanged. It also did not matter whether the study design utilized a crossover or parallel-arm control group, as the results were similar, though the studies that made use of a parallel-arm design exhibited more heterogeneity.
The daily dose of flavanol consumption by the active arm groups in the included studies ranged from 88 to 963 mg. Typical US daily intakes of flavanol are on the order of 20–100 mg (Mink et al., 2007). This raises questions about the usefulness of the information provided by these studies. Consequently, we conducted a subgroup analyses (by dose) that showed a greater reduction in serum LDL concentrations in those studies that administered less than 500 mg of flavanol daily (compared with those that administered ⩾500 mg), though this reduction wasn’t significant. Studies are needed that will provide insight on the optimal daily dose range of flavanol consumption.
Recently, it has been proposed that there may be a discrepancy in the effective dose of flavanols when delivered in a chocolate matrix compared with a beverage mix. A bioavailability study however refutes this, showing that there was no difference in the acute increases in plasma or urinary concentrations of flavanols after consumption of chocolate or dry cocoa containing the same quantities of flavanols (Baba et al., 2000). Nevertheless, when our analyses were restricted to those studies that strictly utilized dark chocolate in the active arm rather than cocoa beverages (Engler et al., 2004; Grassi et al., 2005a; Grassi et al., 2005b; Balzer et al., 2008; Almoosawi et al., 2010), we observed a stronger reduction in serum LDL concentration (−11.31 mg/dl; 95% CI was −20.79 to −1.83 mg/dl).
Further analyses by duration of intake revealed that dark chocolate or cocoa intake has a more meaningful effect on LDL cholesterol levels during a short-term than over a long-term intervention. A possible reason for this may be the fall in compliance that is often associated with studies that go on for a longer duration.
A number of limitations in these analyses merit some comments. There was a wide variation in the amount and formulation of cocoa product used. Many of the studies used quantities of cocoa that may be impractical to recommend for long-term daily consumption (mostly >30 g). In addition, the control diet regimen varied remarkably across studies; this may have contributed to some of the heterogeneity found in the results (I2 statistic was 32% for LDL and 24% for TC) (Figures 2 and 4). Half of the trials included were judged to be of low quality (Jadad score <3). This is largely due to the lack of adequate blinding of subjects and investigators and may have led to some bias. Our review is further limited by the relatively small number of available trials and subjects studied. The generalizability of our study is limited as most of the studies were carried out on healthy subjects. Treatment duration was relatively short; hence, we cannot predict the effects of habitual cocoa intake on lipids over several months to years.
Our analysis however has some strengths. First, most of our results showed relatively very little or no heterogeneity. Second, as with many meta-analyses, by combining information from all relevant studies, we provide more precise and powerful estimates of the effect measure than those derived from the individual studies. Third, we included only randomized controlled trials that followed rigorous protocols and thereby minimized bias.
In conclusion, these current analyses are consistent with beneficial effects of dark chocolate/cocoa product consumption on LDL and neutral effects on TG and HDL in a short-term intervention. Dark chocolate also appears to be a more effective matrix for delivering flavanols than cocoa beverages. Additional studies to evaluate the optimal dose of cocoa consumption and long-term effects of dark chocolate on serum lipids are needed to help appraise the net benefit of dark chocolate consumption on health.
Allen RR, Carson L, Kwik-Uribe C, Evans EM, Erdman Jr JW (2008). Daily consumption of a dark chocolate containing flavanols and added sterol esters affects cardiovascular risk factors in a normotensive population with elevated cholesterol. J Nutr 138, 725–731.
Almoosawi S, Fyfe L, Ho C, Al-Dujaili E (2010). The effect of polyphenol-rich dark chocolate on fasting capillary blood glucose, total cholesterol, blood pressure and glucocorticoids in healthy overweight and obese subjects. Br J Nutr 103, 842–850.
Baba S, Natsume M, Yasuda A, Nakamura Y, Tamura T, Osakabe N et al. (2007). Plasma LDL and HDL cholesterol and oxidized LDL concentrations are altered in normo- and hypercholesterolemic humans after intake of different levels of cocoa powder. J Nutr 137, 1436–1441.
Baba S, Osakabe N, Yasuda A, Natsume M, Takizawa T, Nakamura T et al. (2000). Bioavailability of (-)-epicatechin upon intake of chocolate and cocoa in human volunteers. Free Radic Res 33, 635–641.
Balzer J, Rassaf T, Heiss C, Kleinbongard P, Lauer T, Merx M et al. (2008). Sustained Benefits in Vascular Function through Flavanol-Containing Cocoa in Medicated Diabetic Patients. J Am Coll Cardiol 51, 2141–2149.
Cochrane Collaboration Review Manager. RevMan software version 5.0. Cochrane Collaboration: Oxford, United Kingdom, http://www.cc-ims.net/revman.
Crews WD, Harrison DW, Wright JW (2008). A double-blind placebo-controlled randomized trial of the effects of dark chocolate and cocoa on variables associated with neuropsychological functioning and cardiovascular health: clinical findings from a sample of healthy, cognitively intact older adults. Am J Clin Nutr 87, 872–880.
Davison K, Coates AM, Buckley JD, Howe PR (2008). Effect of cocoa flavanols and exercise on cardiometabolic risk factors in overweight and obese subjects. Int J Obes 32, 1289–1296.
DerSimonian R, Laird N (1986). Meta-analysis in clinical trials. Control Clin Trials 7, 177–188.
Engler MB, Engler MM, Chen CY, Malloy MJ, Browne A, Chiu EY et al. (2004). Mietus-Snyder. Flavonoid-rich Dark chocolate improves endothelial function and increases plasma epicatechin concentrations in Healthy adults. Am Coll Nutr 23, 197–204.
Fraga CG, Actis-Goretta L, Ottaviani JI, Carrasquedo F, Lotito SB, Lazarus S et al. (2005). Regular consumption of flavanol-rich chocolate can improve oxidant stress in young soccer players. Clin Dev Immunol 12, 11–17.
Grassi D, Desideri G, Necozione S, Lippi C, Casale R, Properzi G et al. (2008). Blood pressure is reduced and insulin sensitivity increased in Glucose-intolerant, Hypertensive subjects after 15 days of consuming high-polyphenol dark chocolate. J Nutr 138, 1671–1676.
Grassi D, Lippi C, Necozione S, Desideri G, Ferri C (2005a). Short term administration of dark chocolate is followed by a significant increase in insulin sensitivity and a decrease in blood pressure in healthy persons. Am J Clin Nutr 81, 611–614.
Grassi D, Necozione S, Lippi C, Croce G, Valeri L, Pasqualetti P et al. (2005b). Cocoa reduces blood pressure and insulin resistance and improves endothelium-dependent vasodilation in Hypertensives. Hypertension 46, 398–405.
Hamed MS, Gambert S, Bliden KP, Bailon O, Singla A, Antonino MJ et al. (2008). Dark chocolate effect on platelet activity, C-reactive protein and lipid profile: A pilot study. South Med J 101, 1203–1208.
Hannum SM, Erdman Jr JW (2000). Emerging Health Benefits from Cocoa and Chocolate. J Med Food 3, 73–75.
Hunter JE, Zhang J, Kris-Etherton PM (2010). Cardiovascular disease risk of dietary stearic acid compared with trans, other saturated, and unsaturated fatty acids: a systematic review. Am J Clin Nutr 91, 46–63.
Jadad AR, Moore RA, Carroll D, Jenkinson C, Reynolds DJ, Gavaghan DJ et al. (1996). Assessing the quality of reports of randomized clinical trials: Is blinding necessary? Control Clin Trials 17, 1–12.
Jia L, Liu X, Bai YY, Li SH, Sun K, He C et al. (2010). Short-term effect of cocoa product consumption on lipid profile: a meta-analysis of randomized controlled trials. Am J Clin Nutr 92, 218–225.
Judd JT, Clevidence BA, Muesing RA, Wittes J, Sunkin ME, Podczasy JJ (1994). Dietary trans fatty acids: effects on plasma lipids and lipoproteins of healthy men and women. Am J Clin Nutr 59, 861–868.
Kris-Etherton PM, Derr J, Mitchell DC, Mustad VA, Russell ME, McDonnell ET et al. (1993). The role of fatty acid saturation on plasma lipids, lipoproteins, and apolipoproteins I: effects of whole diets high in cocoa butter, olive oil, soybean oil, dairy butter, and milk chocolate on the plasma lipids of young men. Metabolism 42, 121–129.
Kris-Etherton PM, Derr JA, Mustad VA, Seligson FH, Pearson TA (1994). Effects of a milk chocolate bar per day substituted for a high-carbohydrate snack in young men on an NCEP/AHA Step 1 Diet. Am J Clin Nutr 60 (suppl), 1037S–1042S.
Kris-Etherton PM, Mustad VA (1994). Chocolate feeding studies: a novel approach for evaluating the plasma lipids effects of stearic acid. Am J Clin Nutr 60 (Suppl), 1029S–1036S.
Matsui N, Ito R, Nishimura E, Yoshikawa M, Kato M, Kamei M et al. (2005). Ingested cocoa can prevent high-fat diet–induced obesity by regulating the expression of genes for fatty acid metabolism. Nutrition 21, 594–601.
Mink PJ, Scrafford CG, Barraj LM, Harnack L, Hong C, Nettleton JA et al. (2007). Flavonoid intake and CVD mortality: A prospective study in post-menopausal women. Am J Clin Nutr 85, 895–909.
Muniyappa R, Hall G, Kolodziej TL, Karne RJ, Crandon SK, Quon MJ (2008). Cocoa consumption for 2wks enhances insulin-mediated vasodilation without improving blood pressure or insulin resistance in essential hypertension. Am J Clin Nutr 88, 1685–1696.
Mursu J, Voutilainen S, Nurmi T, Rissanen TH, Virtanen JK, Kaikkonen J et al. (2004). Dark chocolate consumption increases HDL cholesterol concentration and chocolate fatty acids may inhibit lipid peroxidation in healthy humans. Free Radic Biol Med 37, 1351–1359.
Mustad VA, Kris-Etherton PM, Derr J, Reddy CC, Pearson TA (1993). Comparison of the effects of diets rich in stearic acid versus myristic acid and lauric acid on platelet fatty acids excretion of thromboxane A2 and PGI2 metabolites in healthy young men. Metabolism 42, 463–469.
NCEP: Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (1993). Summary of the second report of the National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel II). JAMA 269, 3015–3023.
Polagruto JA, Wang-Polagruto JF, Braun MM, Lee L, Kwik-Uribe C, Keen CL (2006). Cocoa flavanol-enriched snack bars containing phytosterols effectively lower total and low-density lipoprotein cholesterol levels. J Am Diet Assoc 106, 1804–1813.
Taubert D, Roesen R, Lehmann C, Jung N, Schömig E (2007). Effects of low habitual cocoa intake on blood pressure and bioactive nitric oxide- a randomized controlled trial. JAMA 298, 49–60.
Temme EH, Mensink RP, Hornstra G (1996). Comparison of the effects of diets enriched in lauric, palmitic, or oleic acids on serum lipids and lipoproteins in healthy women and men. Am J Clin Nutr 63, 897–903.
Wan Y, Vinson JA, Etherton TD, Proch J, Lazarus SA, Kris-Etherton PM (2001). Effects of cocoa powder and dark chocolate on LDL oxidative susceptibility and prostaglandin concentrations in humans. Am J Clin Nutr 74, 596–602.
Wang-Polagruto JF, Villablanca AC, Polagruto JA, Lee L, Holt RR, Schrader HR et al. (2006). Chronic consumption of flavanol-rich cocoa improves endothelial function and decreases vascular cell adhesion molecule in hypercholesterolemic postmenopausal women. J Cardiovasc Pharmacol 47 (Suppl 2). S177–S186.
We thank Dr Emad Al-Dujaili, Department of Dietetics, Nutrition and Biological Sciences, Queen Margaret University, UK; and Dr PRC Rowe, ATN Center for Metabolic Fitness, School of Health Sciences, University of South Australia, Adelaide, Australia for kindly providing details of their lipid profile measurements upon request by us.
The authors declare no conflict of interest.
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Tokede, O., Gaziano, J. & Djoussé, L. Effects of cocoa products/dark chocolate on serum lipids: a meta-analysis. Eur J Clin Nutr 65, 879–886 (2011). https://doi.org/10.1038/ejcn.2011.64
- dark chocolate
- cardiovascular health
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