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Comprehensive online repository of gut bacteria enzymes

A 3-D illustration of Escherichia coli bacteria found in the intestine. Credit: Christoph Burgstedt / Alamy Stock Photo

Researchers have created an open-access, online database of protein kinases, enzymes that help beneficial gut bacteria to sense and react to environments1. It contains the amino acid sequences of more than 800 enzymes.

The enzymes – also known as probiotic eukaryotic-like serine/threonine kinases (PeSTKs) – regulate the activity of other proteins and participate in cell-to-cell communications.

The data will be useful for sequence-based studies and drug development, says a team at the CSIR-Central Food Technological Research Institute in Mysore.

Friendly bacteria help absorb nutrients and boost the host’s defenses against pathogenic bacteria. To better understand this, the scientists gathered data about PeSTKs of eight helpful bacteria from resources such as SWISS-PROT, Protein Information Resource, Protein Sequence Database, the National Center for Biotechnology Information and UniProt.

The amino acid sequences of 2,500 enzymes were validated using research publications and their references and citations. The researchers, led by Rajagopal Kammara, further scrutinised and sorted these based on validated reports, reducing the data to 830 enzymes.

The enzymes belong to species of eight bacterial genera – Lactobacillus, Bifidobacterium, Enterococcus, Streptococcus, Pediococcus, Leuconostoc, Bacillus and Escherichia.

The database also shows genes, species, length of protein sequences, and lists the amino acid sequences alphabetically.



  1. Lokesh, D. et al. Sci. Data. 9:282 (2022) Doi: 10.1038/s41597-022-01379-2

    PubMed  Article  Google Scholar 

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