• MILESTONES

Editing a cure

Credit: Bloomberg/Rob Waters via Getty Images

Timothy Ray Brown—also known as the ‘Berlin patient’—is the only person ever to be cured of HIV. His story and the research that followed are intimately linked to the discovery, back in 1996, that homozygosity for a CCR5 allele with a 32-base-pair deletion (delta32/delta32) renders some individuals resistant to infection despite exposure through sexual intercourse with HIV-positive partners (MILESTONE 15).

Brown was diagnosed with HIV in 1995 and a decade later underwent allogeneic hematopoietic stem cell transplantation (HSCT) for relapsed acute myeloid leukemia. But the transplant, performed by Gero Hütter of Charité–Universitätsmedizin Berlin, had a twist. Hütter used peripheral blood stem cells from a human leukocyte antigen (HLA)-identical donor that harbored the CCR5 delta32 allele.

The procedure led to complete remission of the cancer and, remarkably, despite the long-lived viral reservoir (MILESTONE 11, 16) being expected to lead to HIV rebound and disease progression during the process of immune reconstitution, no active virus and no viral RNA or proviral DNA could be detected in the blood, bone marrow or rectal mucosa, even almost two years after transplantation and interruption of antiretroviral therapy (ART).

Although at the time of transplant Brown also carried HIV variants tropic for the CXCR4 chemokine receptor, Hütter reasoned that their numbers might have been too low to allow reseeding of the new immune system. A follow-up study showed that Brown’s long-lived CD4+ HIV target cells had been successfully replaced with donor-derived cells. Thus, the CCR5 deletion in donor stem cells and their ability to engraft—killing Brown’s infected cells—may have together contributed to the functional cure of HIV.

Yet, a similar approach subsequently performed in the so-called ‘Boston patients’ proved the latent reservoir of HIV to be far more resilient than previously thought. Timothy Henrich and Daniel Kuritzkes at Brigham and Women’s Hospital in Boston gave HSCT to two HIV-infected men diagnosed with lymphoma, but used donor cells with wild-type CCR5. In this approach, despite a significant reduction in the size of the reservoir following transplant, both patients eventually experienced rebound viremia after cessation of ART.

Still, these landmark studies demonstrated the critical role that CCR5 has in maintaining HIV-1 infection and prompted further research into gene-based therapies to target HIV.

Although genome editing had been proven efficient in rendering T cells refractory to HIV infection, it was not until years later, in 2014, that a team led by Pablo Tebas and Carl June at the University of Pennsylvania in Philadelphia showed this could be done in infected individuals. The clinical trial enrolled 12 patients who were infused with autologous CD4+ T cells modified at the CCR5 locus by zinc-finger nucleases (ZFNs). The patients who stopped ART after cell transfusion exhibited slow viral rebound and proliferation of the modified T cells, indicating enhanced control of the virus. Moreover, one study participant with no viral rebound during ART interruption was found to harbor a single mutated copy of CCR5 and after transfusion a large proportion of this patient’s T cells were resistant to HIV.

Together, these findings demonstrated that gene-targeting approaches could provide a safer and more practical approach than the risky and restrictive HSCT and opened the door for ZFNs and other gene-editing methods, such as TALENs and CRISPR, to be exploited in the targeting of latently infected cells.

Although many challenges remain, these groundbreaking discoveries, together with those on early ART initiation (MILESTONE 20, 21), broadly neutralizing antibodies (MILESTONE 19) and new-generation latency-reversing agents, are paving the way toward the development of a broad-scale and safe strategy for the complete eradication of HIV or its control in the absence of lifelong therapy.

References

  1. 1.

    Hütter, G. et al. Long-term control of HIV by CCR5 delta32/delta32 stem-cell transplantation. N. Engl. J. Med. 360, 692–698 (2009).

    PubMed  Article  Google Scholar 

  2. 2.

    Allers, K. et al. Evidence for the cure of HIV infection by CCR5D32/D32 stem cell transplantation. Blood 117, 2791–2799 (2011).

    PubMed  Article  Google Scholar 

  3. 3.

    Henrich, T. J. et al. Long-term reduction in peripheral blood HIV type 1 reservoirs following reduced-intensity conditioning allogeneic stem cell transplantation. J. Infect. Dis. 207, 1694–1702 (2013).

    PubMed  Article  Google Scholar 

  4. 4.

    Henrich, T. J. et al. Antiretroviral-free HIV-1 remission and viral rebound after allogeneic stem cell transplantation: report of 2 cases. Ann. Intern. Med. 161, 319–327 (2014).

    PubMed  Article  Google Scholar 

  5. 5.

    Tebas, P. et al. Gene editing of CCR5 in autologous CD4 T cells of persons infected with HIV. N. Engl. J. Med. 370, 901–910 (2014).

    PubMed  Article  Google Scholar 

Download references

Nature Careers

Jobs

Nature Briefing

An essential round-up of science news, opinion and analysis, delivered to your inbox every weekday.

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing