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In pictures: details revealed with advanced SEM

© Hitachi High-Technologies

The fascinatingly diverse detail of a rat’s bronchial epithelial surface. Long, hair-like structures, known as ciliated cells, are important for the bronchi’s self-clearing mechanisms. Large, round extrusions, known as goblet cells, secrete mucous to catch dust, allergens and pathogens. Smaller rounded extrusions are brush cells — chemosensors that detect irritants. This type of imaging has been used to study the effects of malaria and smoking on the lungs.

technical note

This image was taken at 10 kV, the middle setting of three acceleration voltage options on the TM4000. The setting determines the depth that electrons penetrate the specimen’s surface. (SEM model: TM4000)

© Hitachi High-Technologies

This image shows 50 µm of solar panel surface and highlights the pyramidal structures that help trap light and reduce reflection. Photovoltaic researchers are seeking ways to optimize the texture of these surfaces, since the shape, size and uniformity of the pyramids affect optical reflectance and energy capture.

Technical note

The acceleration voltage of an SEM controls how far electrons penetrate a sample. The higher the acceleration voltage, the deeper electrons will penetrate. With higher acceleration voltages, ultrastructural information from deeper layers may affect the view of the surface morphology. This image was taken at a relatively low acceleration voltage of 5 kV. (SEM model: FlexSEM 1000 II)

© Hitachi High-Technologies

A 2 mm bee head. Honey bees have been the subject of intense research, as their numbers are declining due to the spread of varroa mites (Varroa destructor and V. jacobsoni). The mite attaches to hive larvae or the body of a bee and weakens the larvae or bee by sucking out fat bodies. Symptoms include low body weight and deformed wings, both of which have been studied using SEM imaging.

Technical Note

SEM imaging can achieve higher resolutions and magnifications than dissection microscopes. The specimen in the above image was relatively unaltered, as it was imaged in a low-vacuum environment, which doesn’t demand the specimen must be dehydrated, frozen or chemically fixed, unlike for other types of SEMs. Low-vacuum scanning electron microscopy protects samples and streamlines the imaging process, a benefit for studies that involve repetitive phenotypic analysis of insects, such as taxonomic studies, quantitative genetic studies, and mutant screens. (SEM model: TM4000)

© Hitachi High-Technologies

This is what 20 µm of foundation from the make-up industry looks like. A typical product like this contains 15 to 50 ingredients. The most common minerals used as a base for foundation are mica, bismuth oxychloride, titanium dioxide and zinc oxide. The spheres here are probably silica, which acts as an anticaking agent, bulking agent, opacifying agent and suspending agent. Silica also typically absorbs sweat and oil, reduces light reflection and improves spreadability.

Technical Note

With SEMs becoming increasingly compact and easy to use, the cosmetics and healthcare industries are widely adopting the technology. (SEM model: TM3030)

© Hitachi High-Technologies

An abalone shell made up of 0.5 µm thick tiles of calcium carbonate, or chalk, cemented together by a protein coating. The whole structure is 3,000 times more resistant to fracturing than a crystal of calcium carbonate alone. This property comes from the positive charge of the protein coating that binds to the negative charge of the hexagonal calcium carbonate tiles. This binding is weak enough that the layers can slide slightly apart to absorb the energy of a blow. Material scientists interested in biomimicry are seeking to replicate this structure to create tougher materials.

Technical Note

SEMs have greater depth of focus than optical microscopes, which makes it easier for scientists to clarify the structure of an entire specimen with a high surface roughness. (SEM model: FlexSEM 1000 II)

© Hitachi High-Technologies

A hydrogen-absorbing alloy magnified 30,000 times. Hydrogen-storage alloys are metallic materials that can reversibly absorb and release hydrogen from the gas phase or electrochemically. These alloys are already being used in electrodes, particularly in electric vehicles, to improve performance and to avoid using common alternative materials that contain toxic lead or cadmium, as these can leach into landfill.

Technical Note

The increasing ease of SEM imaging has seen a recent expansion in its use for quality control of electronic components. (SEM model: FlexSEM 1000 II)

© Hitachi High-Technologies

An image of a 300 µm region of a Peyer’s patch, a small mass of lymphatic tissue. These patches are found mostly throughout the ileum region of the small intestine, and are important to the function of the immune system. They also play a role in determining which substances must be dealt with as foreign to the gut, although this process is still little understood.

Technical Note

The low-vacuum conditions of the FlexSEM 1000 II make it possible to observe water- or oil-containing medical specimens without preprocessing. (SEM model: FlexSEM 1000 II)

© Hitachi High-Technologies

An image of a 100 µm region of a mould, featuring a sporangium, its main reproductive organ. Once released, if the small, round spores in the image land in an optimum position, they will grow root structures known as hyphae and generate more mould. This mould grew on bread.

Technical Note

Food researchers wishing to study sterilization processes can compare the condition of spores and mould before and after sterilization. Researchers and industry experts look for lyses (disintegration) or the presence of live mould on food or packaging. (SEM model: TM3030)

© Hitachi High-Technologies

A mast cell imaged at a magnification of 15,000. These cells are a type of white blood cell found in connective tissue. The small spherical shapes in this image contain chemical mediators, including histamine and heparin. These play a key role in immune system reactions. When a mast cell is activated during an allergic reaction or in response to injury or inflammation, these mediators are released into tissues.

Technical Note

This is an image created using an acceleration voltage of 10 kV, similar to that used for the image of the rat bronchi, but this image is at more than twice the magnification. (SEM model: TM4000)

© Hitachi High-Technologies

An image of 100 µm-sized region of a water-absorbing polymer. These polymers are useful in cleaning and hygiene products. SEM images can be used to measure what happens to these molecules at different saturation points.

Technical Note

This image was taken at a relatively low 3 kV, but the FlexSEM 1000 II employs an Opti-Bias system that increases the emission current at low acceleration voltages to optimize the brightness of an image. (SEM model: FlexSEM 1000 II)

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